PCR Detection Solutions for Coronavirus Powered by Bioz PCR Detection Solutions for Coronavirus Price USD$269.00 SKU 28113 Format RT-PCR Master Mix Size 100 Reactions 200 Reactions 500 Reactions Quantity Overview Details Documentation Citations Overview COVID-19 detection based on the assays and protocols developed by the CDC Primer & Probe Mixes contain all 3 CDC developed assays in individual tubes All assays are premixed to the working concentrations recommended by the CDC Positive Control contains 2 nCoV nucleocapsid target gene RNA (N1,N2) and RNase P (internal control) which are compatible with the CDC primer/probe set Convenient ready-to-use 2X One-Step RT-PCR Master Mix Manufactured under ISO 13485:2016 for research use only Accurate diagnosis of infection with 2019-nCoV is through the use of real-time RT PCR (rRT-PCR) assays for the in vitro qualitative detection of 2019-Novel Coronavirus (2019-nCoV) in respiratory specimens and sera. The CDC designed and released RT-PCR assays and published a protocol for the detection of the 2019-nCoV (now being referred to as COVID-19). Norgen Biotek is offering these CDC assays to researchers for the identification of the virus in our 2019-nCoV Primer & Probe Mixes (Cat. TM67101). Norgen’s 2019-nCoV Primer & Probe Mixes (Cat. TM67101) includes all 3 CDC developed assays in individual tubes, which are premixed to the working concentrations recommended by the CDC. These assays are manufactured under Norgen’s ISO 13485:2016 quality system and are for research use only. In addition to 2019-nCoV Primer & Probe Mixes, Norgen is also offering a 2019-nCoV RT-PCR Positive Control (Cat. PC67102). The control plasmid contains the CDC 2019-nCoV markers (N1,N2) and RNase P gene which are compatible with the CDC 2019-nCoV specific primer/probe sets. Norgen also offers our 2X One-Step RT-PCR Master Mix (Cat. 28113) as part of the complete detection system. Please refer to the CDC website for the most up-to-date protocol for use of the 2019-nCoV Primer & Probe Mixes and 2019-nCoV RT-PCR Positive Control ( https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html ). Please note, Norgen Biotek is not an endorsed supplier of the CDC coronavirus RT-PCR panel or positive controls. Norgen is offering these products based on the sequences that were published by the CDC, and these are being offered to our customers for research use only, not for in vitro diagnostic use Details Supporting Data Previous Figure 1. Validation of Norgen's 2019-nCoV Positive Control (Cat. PC67102) and 2X One-Step RT-PCR Master Mix (Cat. 28113) with the 2019-nCoV RT-PCR Kit (Cat. TM67100). A. One-Step RT-PCR reactions were prepared according to the CDC Real-Time RT-PCR Panel for Detection 2019-Novel Coronavirus manual. Five µL of Norgen's Positive Control (2x 10^6 theoretical copy number) was used for a 25 µL PCR reaction. Each target indicated by the name was successfully detected including the internal control (RP). B. The theoretical detection limit was estimated by serially diluting Norgen's Positive Control and testing with 2X One-Step RT-PCR Master Mix. PCR detection was sensitive and could detect down to 20 copies within 35 Ct. in a Bio-Rad real-time PCR system. Next Click for expanded view Reagents supplied – Cat # 28113 2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions Storage Conditions and Product Stability 2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year. Documentation PROTOCOLS (PC67102, PC67120) 2019-nCoV Positive Control - Protocol (50 and 500 µL) (TM67101, TM67130) 2019-nCoV Primer & Probe Mixes - Protocol (50 and 500 rxns) Related Products 2019-nCoV TaqMan RT-PCR Kit Total RNA Purification Kit Swab Collection and Total Nucleic Acid Preservation System Saliva RNA Collection and Preservation Devices
PCR Detection Solutions for Coronavirus Powered by Bioz PCR Detection Solutions for Coronavirus Price USD$269.00 SKU 28113 Format RT-PCR Master Mix Size 100 Reactions 200 Reactions 500 Reactions Quantity
