50 bp DNA Ladder
Ideal for PCR product size determination
For research use only and NOT intended for in vitro diagnostics.
50 bp DNA Ladder
Ideal for PCR product size determination
SKU
13525
Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- 10 discrete bands from 50 bp to 500 bp
- Higher intensity reference band at 250 bp
The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
50 bp DNA Ladder (Cat# 13525) - 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
500
|
79
|
|
2
|
450
|
67
|
|
3
|
400 |
59
|
|
4
|
350 |
55
|
|
5
|
300 |
49
|
|
6
|
250 |
76
|
|
7
|
200 |
33
|
|
8
|
150 |
22
|
|
9
|
100
|
31
|
|
10
|
50 |
29
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Fluorescent carbon nanoparticle-based lateral flow biosensor for ultrasensitive detection of DNA |
| Citation | Biosensors and Bioelectronics 2023. |
| Authors | Takalkar, S., Baryeh, K., & Liu, G. (2017). |
| Title | Inter-simple Sequence Repeat (ISSR) Analysis of Somaclonal Variation in Artemisia amygdalina Decne, plantlets Regenerated from nodal explants |
| Citation | Journal on New Biological Reports 2023. |
| Authors | M. Khan, B.A. Ganai, A.N. Kamili, M. Akbar |
| Title | Microsatellite instability at three microsatellite loci (D6mit3, D9mit2 and D15Mgh1) located in different common fragile sites of rats exposed to cadmium |
| Citation | Mutation Research 696 (2010) 160-166 2023. |
| Authors | Akmal A. El-Ghora, Magda M. Noshya, Hassan M. El Ashmaouib, Jehane I. Eida,*, Mohamed S. Hassananeb |
| Title | Two Novel Variants Affecting CDKL5 Transcript Associated with Epileptic Encephalopathy |
| Citation | Genetic Testing and Molecular Biomarkers 2023. |
| Authors | Neupauerová, J., Šterbová, K., Vlcková, M., Sebronová, V., Maríková, T. Á., Krutová, M., ... & Laššuthová, P. (2017). |