50 bp DNA Ladder Ideal for PCR product size determination For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 50 bp DNA Ladder Ideal for PCR product size determination Price USD$75.00 SKU 13525 Size 100 Loads Quantity Overview Details Documentation Citations Overview Ready-to-useQuantitativeHighly StablePrecise10 discrete bands from 50 bp to 500 bpHigher intensity reference band at 250 bp The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR productsContents1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS). Details Supporting Data Previous Figure 1: 50 bp DNA Ladder Next Click for expanded view 50 bp DNA Ladder (Cat# 13525) - 100 loads Ladder Properties: • Ten discrete bands, ranging from 50 bp to 500 bp • Higher intensity band at 250 bp for easy reference Fragment Size (bp) Mass (ng) 1 500 79 2 450 67 3 400 59 4 350 55 5 300 49 6 250 76 7 200 33 8 150 22 9 100 31 10 50 29 Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well. Storage: Stable at room temperature. For longer term storage, -20°C is recommended. This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer. Documentation PROTOCOLS 13525 - Product Insert APPLICATION NOTES DNA Quantification by Gel Densitometry with Norgen DNA Ladders SAFETY DATA SHEETS 50 bp DNA Ladder - MSDS Citations Title Inter-simple Sequence Repeat (ISSR) Analysis of Somaclonal Variation in Artemisia amygdalina Decne. plantlets Regenerated from nodal explants. Journal Journal on New Biological Reports. 2013. Authors Khan M, Ganai B, Kamili A, Akbar M Title Lead acetate and arsenic trioxide induce instability of microsatellites at three different fragile sites (6q21, 9q32-9q33 and 15p14) within the genome of the rat. Journal Mutation Research. 2011. Authors El-Ghor A, Noshy M, Eid J. Title Microsatellite instability at three microsatellite loci (D6mit3, D9mit2 and D15Mgh1) located in different common fragile sites of rats exposed to cadmium. Journal Mutation Research. 2010. Authors El-Ghor AA, Noshy MM, El Ashmaoui HM, Eid JI, Hassanane MS.
50 bp DNA Ladder Ideal for PCR product size determination For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 50 bp DNA Ladder Ideal for PCR product size determination Price USD$75.00 SKU 13525 Size 100 Loads Quantity
