Ideal for PCR product size determination

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • 10 discrete bands from 50 bp to 500 bp
  • Higher intensity reference band at 250 bp

ItemsCat. #Size
50 bp DNA Ladder 13525 100 loads

50 bp DNA Ladder

The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

50 bp DNA Ladder (Cat# 13525) - 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference

 

Fragment
Size (bp)
Mass (ng)
1
 500
 79
2
 450
 67
3
 400
 59
4
 350
 55
5
 300
 49
6
 250
 76
7
 200
 33
8
 150
 22
9
 100
 31
10
 50
 29
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
 
Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.