Ad5 Type C Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Ad5 Type C Detection Kits Price USD$699.00 SKU TM42650 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for the Ad5 Type C Available in TaqMan format for analysis Adenoviruses are double-stranded DNA, non-enveloped icosahedral viruses with a size of 60-90 nm. Adenoviruses represent the largest non-enveloped viruses (around 30-38Kbp) with a unique fiber associated with each penton base of the capsid that helps in its attachment to the host cell via the coxsackie-adenovirus receptor on the surface of the host cell. There are 51 immunologically distinct human adenovirus serotypes that can cause human infections ranging from respiratory disease (mainly species HAdV-B and C), and conjunctivitis (HAdV-B and D), to gastroenteritis (HAdV-F serotypes 40 and 41). Adenoviruses are unusually stable to chemical or physical agents and adverse pH conditions, allowing for prolonged survival outside of the body and water. Adenoviruses are primarily spread via respiratory droplets, however they can also be spread by fecal routes as well. Human Adenovirus Type C species contains 4 different serotypes: 1, 2, 5 and 6, and these adenoviruses are known to cause respiratory infections in humans. TaqMan PCR Kit Click to expand options Ad5 Type C TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Ad5 Type C TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM42650 (100 rxns) Cat. TM42610 (100 rxns) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Ad5 Type C Primer & Probe Mix 280 μL 280 μL Ad5 Type C Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation APPLICATION NOTES The Isolation of High Quality Stool DNA and its Application to Quantitative Adenovirus Detection using TaqMan® Real-Time PCR SAFETY DATA SHEETS TM42600 - Safety Data Sheet
Ad5 Type C Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Ad5 Type C Detection Kits Price USD$699.00 SKU TM42650 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
