Anisakis spp. TaqMan PCR Detection Kit

Overview

Anisakis spp. are parasitic nematodes that have life cycles involving fish and marine mammals and capable of stimulating fish-borne illnesses called Anisakiasis. Anisakiasis is a human parasitic infection of the gastrointestinal tract caused by the consumption of raw or undercooked seafood containing larvae of the nematode Anisakis ssp. The symptoms are that the patient will experience severe abdominal pain, nausea, vomiting, or diarrhea within 48 h after consumption. A more severe case is where the Anisakis spp. have infected the hosts by attaching themselves to the intestinal mucosa and penetrate the gastrointestinal tract. This severe infection of the mucosa can lead to tissue damage and gastric ulcers. Anisakiasis can be easily prevented by adequate cooking at temperatures greater than 60 °C or freezing. The FDA recommends all shellfish and fish intended for raw consumption be blast frozen to −35 °C or below for 15 hours or be regularly frozen to −20 °C or below for seven days.

Norgen’s Anisakis spp. TaqMan PCR Kit is designed for the detection of Anisakis spp. specific DNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Anisakis spp. specific DNA is based on TaqMan PCR providing a simple, reliable and rapid result for the detection of Anisakis spp. infection. Norgen’s Anisakis spp. TaqMan PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Anisakis spp. TaqMan PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

Norgen’s Anisakis spp. TaqMan PCR Kit was developed and validated to be used with the following PCR instruments:

• Qiagen Rotor-Gene Q
• BioRad CFX96 Touch™ Real-Time PCR Detection System
• QuantStudio™ 7 Pro Real-Time PCR System

Details

Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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