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For the rapid preparation of genomic DNA from biofilm
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Maximum Column Binding Capacity |
50 µg
|
Maximum Column Loading Volume |
650 µL
|
Minimum Biofilm Input (wet weight) | 50 mg |
Maximum Biofilm Input (wet weight) |
200 mg
|
Average Yield from 200 mg of biofilm* |
2-4 µg
|
Time to Complete 10 Purifications | 45 minutes |
Supporting Data
Component | Cat. 62300 (50 preps) |
---|---|
Lysis Buffer L | 30 mL |
Lysis Additive A | 6 mL |
Binding Buffer I | 7 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 8 mL |
Bead Tubes | 50 |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Title | From rivers to marine environments: A constantly evolving microbial community within the plastisphere |
Journal | Marine Pollution Bulletin. 2022. |
Authors | Alice Delacuvellerie, Tosca Ballerini, Laura Fr`ere, Sabine Matallana-Surget, Bruno Dumontet, Ruddy Wattiez |
Title | New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea☆ |
Journal | Environmental Pollution. 2022. |
Authors | A. Delacuvellerie, A. G´eron, S. Gobert, R. Wattiez a. |
Title | Comprehensive characterization of a cost-effective microbial fuel cell with Pt-free catalyst cathode and slip-casted ceramic membrane |
Journal | International Journal of Hydrogen Energy. 2021. |
Authors | RodrÃguez, J., Mais, L., Campana, R., Piroddi, L., Mascia, M., Gurauskis, J., Vacca, A., & Palmas, S. |
Biofilm DNA Isolation Kit
Norgen’s Biofilm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from biofilm. Genomic DNA is efficiently extracted from the biofilm by a combination of heat treatment, detergents and the use of provided Bead Tubes. The kit is able to remove known PCR inhibitors typically found within the biofilm, including humic acid. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR and Southern Blot analysis. Preparation time for a single sample is less than 45 minutes, and each kit contains sufficient materials for 50 preparations.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Maximum Column Binding Capacity |
50 µg
|
Maximum Column Loading Volume |
650 µL
|
Minimum Biofilm Input (wet weight) | 50 mg |
Maximum Biofilm Input (wet weight) |
200 mg
|
Average Yield from 200 mg of biofilm* |
2-4 µg
|
Time to Complete 10 Purifications | 45 minutes |
Component | Cat. 62300 (50 preps) |
---|---|
Lysis Buffer L | 30 mL |
Lysis Additive A | 6 mL |
Binding Buffer I | 7 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 8 mL |
Bead Tubes | 50 |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Title | From rivers to marine environments: A constantly evolving microbial community within the plastisphere |
Journal | Marine Pollution Bulletin. 2022. |
Authors | Alice Delacuvellerie, Tosca Ballerini, Laura Fr`ere, Sabine Matallana-Surget, Bruno Dumontet, Ruddy Wattiez |
Title | New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea☆ |
Journal | Environmental Pollution. 2022. |
Authors | A. Delacuvellerie, A. G´eron, S. Gobert, R. Wattiez a. |
Title | Comprehensive characterization of a cost-effective microbial fuel cell with Pt-free catalyst cathode and slip-casted ceramic membrane |
Journal | International Journal of Hydrogen Energy. 2021. |
Authors | RodrÃguez, J., Mais, L., Campana, R., Piroddi, L., Mascia, M., Gurauskis, J., Vacca, A., & Palmas, S. |