Biofilm DNA Isolation Kit

Cat. 62300
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Biofilm DNA Isolation Kit

Cat. 62300

For the rapid preparation of genomic DNA from biofilm

  • Rapid and convenient method to isolate genomic DNA from different types of biofilm and biofilm forming-bacteria
  • No phenol or chloroform extractions
  • Yields high quality DNA that is ready for PCR and other downstream applications

For research use only and NOT intended for in vitro diagnostics.

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Biofilm DNA Isolation Kit
(Cat. 62300)
50 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 62300
Kit Specifications
Maximum Column Binding Capacity
50 µg
Maximum Column Loading Volume
650 µL
Minimum Biofilm Input (wet weight) 50 mg
Maximum Biofilm Input (wet weight)
200 mg
Average Yield from 200 mg of biofilm*
2-4 µg
Time to Complete 10 Purifications 45 minutes
* Yield will vary depending on the type of biofilm sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 62300 (50 preps)
Lysis Buffer L 30 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title From rivers to marine environments: A constantly evolving microbial community within the plastisphere
Journal Marine Pollution Bulletin. 2022.
Authors Alice Delacuvellerie, Tosca Ballerini, Laura Fr`ere, Sabine Matallana-Surget, Bruno Dumontet, Ruddy Wattiez
Title New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea☆
Journal Environmental Pollution. 2022.
Authors A. Delacuvellerie, A. G´eron, S. Gobert, R. Wattiez a.
Title Comprehensive characterization of a cost-effective microbial fuel cell with Pt-free catalyst cathode and slip-casted ceramic membrane
Journal International Journal of Hydrogen Energy. 2021.
Authors Rodríguez, J., Mais, L., Campana, R., Piroddi, L., Mascia, M., Gurauskis, J., Vacca, A., & Palmas, S.

Biofilm DNA Isolation Kit

Norgen’s Biofilm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from biofilm. Genomic DNA is efficiently extracted from the biofilm by a combination of heat treatment, detergents and the use of provided Bead Tubes. The kit is able to remove known PCR inhibitors typically found within the biofilm, including humic acid. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR and Southern Blot analysis. Preparation time for a single sample is less than 45 minutes, and each kit contains sufficient materials for 50 preparations.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 62300
Kit Specifications
Maximum Column Binding Capacity
50 µg
Maximum Column Loading Volume
650 µL
Minimum Biofilm Input (wet weight) 50 mg
Maximum Biofilm Input (wet weight)
200 mg
Average Yield from 200 mg of biofilm*
2-4 µg
Time to Complete 10 Purifications 45 minutes
* Yield will vary depending on the type of biofilm sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component Cat. 62300 (50 preps)
Lysis Buffer L 30 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title From rivers to marine environments: A constantly evolving microbial community within the plastisphere
Journal Marine Pollution Bulletin. 2022.
Authors Alice Delacuvellerie, Tosca Ballerini, Laura Fr`ere, Sabine Matallana-Surget, Bruno Dumontet, Ruddy Wattiez
Title New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea☆
Journal Environmental Pollution. 2022.
Authors A. Delacuvellerie, A. G´eron, S. Gobert, R. Wattiez a.
Title Comprehensive characterization of a cost-effective microbial fuel cell with Pt-free catalyst cathode and slip-casted ceramic membrane
Journal International Journal of Hydrogen Energy. 2021.
Authors Rodríguez, J., Mais, L., Campana, R., Piroddi, L., Mascia, M., Gurauskis, J., Vacca, A., & Palmas, S.

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