• Rapid and convenient method to isolate genomic DNA from different types of biofilm and biofilm forming-bacteria
  • No phenol or chloroform extractions
  • Yields high quality DNA that is ready for PCR and other downstream applications

ItemsCat. #Size
Biofilm DNA Isolation Kit 62300 50 preps

Biofilm DNA Isolation Kit

Norgen’s Biofilm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from biofilm. Genomic DNA is efficiently extracted from the biofilm by a combination of heat treatment, detergents and the use of provided Bead Tubes. The kit is able to remove known PCR inhibitors typically found within the biofilm, including humic acid. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR and Southern Blot analysis. Preparation time for a single sample is less than 45 minutes, and each kit contains sufficient materials for 50 preparations.

Kit Specifications
Maximum Column Binding Capacity
50 µg
Maximum Column Loading Volume
650 µL
Minimum Biofilm Input (wet weight) 50 mg
Maximum Biofilm Input (wet weight)
200 mg
Average Yield from 200 mg of biofilm*
2-4 µg
Time to Complete 10 Purifications 45 minutes
* Yield will vary depending on the type of biofilm sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.


Kit Components
Component Cat. 62300 (50 preps)
Lysis Buffer L 30 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1