Biofilm DNA Isolation Kit (Cat. 62300)
Biofilm DNA Isolation Kit
Cat. 62300
![Biofilm DNA Isolation Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/62300-Reflection-web.jpg?itok=PBmgcJmz" "Biofilm DNA Isolation Kit")
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Biofilm DNA Isolation Kit
Cat. 62300
For the rapid preparation of genomic DNA from biofilm
- Rapid and convenient method to isolate genomic DNA from different types of biofilm and biofilm forming-bacteria
- No phenol or chloroform extractions
- Yields high quality DNA that is ready for PCR and other downstream applications
For research use only and NOT intended for in vitro diagnostics.
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Biofilm DNA Isolation Kit
(Cat. 62300) 50 preps
(Cat. 62300) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
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Kit Specifications
You have selected: Cat. 62300
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
50 µg
|
| Maximum Column Loading Volume |
650 µL
|
| Minimum Biofilm Input (wet weight) | 50 mg |
| Maximum Biofilm Input (wet weight) |
200 mg
|
| Average Yield from 200 mg of biofilm* |
2-4 µg
|
| Time to Complete 10 Purifications | 45 minutes |
* Yield will vary depending on the type of biofilm sample processed
​
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Supporting Data
Click for expanded view
| Component | Cat. 62300 (50 preps) |
|---|---|
| Lysis Buffer L | 30 mL |
| Lysis Additive A | 6 mL |
| Binding Buffer I | 7 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Bead Tubes | 50 |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Safety Data Sheets:
Product Flyers:
| Title | From rivers to marine environments: A constantly evolving microbial community within the plastisphere |
| Journal | Marine Pollution Bulletin. 2022. |
| Authors | Alice Delacuvellerie, Tosca Ballerini, Laura Fr`ere, Sabine Matallana-Surget, Bruno Dumontet, Ruddy Wattiez |
| Title | New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea☆ |
| Journal | Environmental Pollution. 2022. |
| Authors | A. Delacuvellerie, A. G´eron, S. Gobert, R. Wattiez a. |
| Title | Comprehensive characterization of a cost-effective microbial fuel cell with Pt-free catalyst cathode and slip-casted ceramic membrane |
| Journal | International Journal of Hydrogen Energy. 2021. |
| Authors | RodrÃguez, J., Mais, L., Campana, R., Piroddi, L., Mascia, M., Gurauskis, J., Vacca, A., & Palmas, S. |
Biofilm DNA Isolation Kit
Norgen’s Biofilm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from biofilm. Genomic DNA is efficiently extracted from the biofilm by a combination of heat treatment, detergents and the use of provided Bead Tubes. The kit is able to remove known PCR inhibitors typically found within the biofilm, including humic acid. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR and Southern Blot analysis. Preparation time for a single sample is less than 45 minutes, and each kit contains sufficient materials for 50 preparations.
Supporting Data
Click for expanded view
Kit Specifications
You have selected: Cat. 62300
|
Kit Specifications
|
|
| Maximum Column Binding Capacity |
50 µg
|
| Maximum Column Loading Volume |
650 µL
|
| Minimum Biofilm Input (wet weight) | 50 mg |
| Maximum Biofilm Input (wet weight) |
200 mg
|
| Average Yield from 200 mg of biofilm* |
2-4 µg
|
| Time to Complete 10 Purifications | 45 minutes |
* Yield will vary depending on the type of biofilm sample processed
​
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 62300 (50 preps) |
|---|---|
| Lysis Buffer L | 30 mL |
| Lysis Additive A | 6 mL |
| Binding Buffer I | 7 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Bead Tubes | 50 |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Safety Data Sheets:
Product Flyers:
| Title | From rivers to marine environments: A constantly evolving microbial community within the plastisphere |
| Journal | Marine Pollution Bulletin. 2022. |
| Authors | Alice Delacuvellerie, Tosca Ballerini, Laura Fr`ere, Sabine Matallana-Surget, Bruno Dumontet, Ruddy Wattiez |
| Title | New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea☆ |
| Journal | Environmental Pollution. 2022. |
| Authors | A. Delacuvellerie, A. G´eron, S. Gobert, R. Wattiez a. |
| Title | Comprehensive characterization of a cost-effective microbial fuel cell with Pt-free catalyst cathode and slip-casted ceramic membrane |
| Journal | International Journal of Hydrogen Energy. 2021. |
| Authors | RodrÃguez, J., Mais, L., Campana, R., Piroddi, L., Mascia, M., Gurauskis, J., Vacca, A., & Palmas, S. |
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