Biofilm DNA Isolation Kit

Overview

Rapid and convenient method to isolate genomic DNA from different types of biofilm and biofilm forming-bacteria
No phenol or chloroform extractions
Yields high quality DNA that is ready for PCR and other downstream applications

Norgen’s Biofilm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from biofilm. Genomic DNA is efficiently extracted from the biofilm by a combination of heat treatment, detergents and the use of provided Bead Tubes. The kit is able to remove known PCR inhibitors typically found within the biofilm, including humic acid. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR and Southern Blot analysis. Preparation time for a single sample is less than 45 minutes, and each kit contains sufficient materials for 50 preparations.

Details

Supporting Data

Figure 1. High quality and yield of DNA from biofilm formed by two different species, Komagataeibacter xylinus (1-4), Komagataeibacter hansenii (5-8). Total DNA was isolated from 200 mg of biofilm using Norgen's Biofilm DNA Isolation Kit. For evaluation, 10 µL of each 100 µL DNA elution was run on a 1.2 % agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M: Norgen's HighRanger 1 kb DNA Ladder (cat. 11900).

Figure 2. High DNA quality. DNA was isolated from a number of bioflim using Norgen's Biofilm DNA Isolation Kit. All DNA elutions isolated using Norgen’s Biofilm DNA Isolation Kit showed an excellent DNA quality indicating the consistent and robust performance of the Biofilm DNA Isolation Kit.

Figure 3. Amplification of bacterial cellulose synthesis (bcs), a gene from Komagataeibacter xylinus (circle) and Komagataeibacter hansenii (cross). From 100 µL elution, 5 µL of biofilm DNA was directly mixed to real-time PCR master mix (total reaction volume 20 µL), and the real-time SYBR Green PCR reaction was performed. The bcs gene was successfully amplified from the different samples without any inhibition and at the same Ct, indicating the excellent quality of biofilm DNA for downstream applications.

Kit Specifications
Maximum Column Binding Capacity	50 µg
Maximum Column Loading Volume	650 µL
Minimum Biofilm Input (wet weight)	50 mg
Maximum Biofilm Input (wet weight)	200 mg
Average Yield from 200 mg of biofilm*	2-4 µg
Time to Complete 10 Purifications	45 minutes

* Yield will vary depending on the type of biofilm sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 62300 (50 preps)
Lysis Buffer L	30 mL
Lysis Additive A	6 mL
Binding Buffer I	7 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Bead Tubes	50
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Citations

Title	From rivers to marine environments: A constantly evolving microbial community within the plastisphere
Journal	Marine Pollution Bulletin. 2022.
Authors	Alice Delacuvellerie, Tosca Ballerini, Laura Fr`ere, Sabine Matallana-Surget, Bruno Dumontet, Ruddy Wattiez

Title	New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea☆
Journal	Environmental Pollution. 2022.
Authors	A. Delacuvellerie, A. G´eron, S. Gobert, R. Wattiez a.

Title	Comprehensive characterization of a cost-effective microbial fuel cell with Pt-free catalyst cathode and slip-casted ceramic membrane
Journal	International Journal of Hydrogen Energy. 2021.
Authors	Rodríguez, J., Mais, L., Campana, R., Piroddi, L., Mascia, M., Gurauskis, J., Vacca, A., & Palmas, S.

Receive Free Shipping and More When You Purchase Eligible Stool Products. See Promo