Blood DNA Isolation Kits

Cat. 46300, 46380, 51400, 31200, 46350, 59800, 62600

Blood DNA Isolation Kit (Magnetic Bead System)

Blood DNA Isolation Kit (Magnetic Bead System) top view

Blood DNA Isolation 96-Well Kit (Magnetic Bead System)

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Blood DNA Isolation Kits

Cat. 46300, 46380, 51400, 31200, 46350, 59800, 62600

For the rapid preparation of high quality DNA from whole blood

High yield and high purity DNA ready for any application
Available in a variety of formats to properly suit your needs
Compatible with blood collected on a variety of commercially available tubes

For research use only and NOT intended for in vitro diagnostics.

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Blood DNA Isolation Mini Kit
(Cat. 46300) 50 Preps

Blood DNA Isolation Mini Kit
(Cat. 46380) 100 preps

Blood DNA Isolation Midi Kit
(Cat. 51400) 20 Preps

Blood DNA Isolation Maxi Kit
(Cat. 31200) 12 Preps

Blood DNA Isolation 96-Well Kit
(Cat. 46350) 2 x 96-well plates

Blood DNA Isolation Kit (Magnetic Bead System)
(Cat. 59800) 50 preps

Blood DNA Isolation 96-Well Kit (Magnetic Bead System)
(Cat. 62600) 2 x 96-well plates

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

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Kit Specifications

You have selected: Cat. 46300

Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

You have selected: Cat. 46380

Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

You have selected: Cat. 51400

Kit Specifications
Minimum Blood Input	0.3 mL
Maximum Blood Input	2 mL
Column Binding Capacity	> 100 µg
Average Yield	60 µg*
Elution Volume	200-300 uL
Analyte Purified	Genomic DNA, mitochondrial DNA, viral DNA, bacterial DNA
Time to Complete 10 Purifications	50-60 minutes

*Yield will vary depending on the type of blood processed

You have selected: Cat. 31200

Kit Specifications
Maximum Blood Input	10 mL
Column Binding Capacity	> 500 µg
Average Yield	200-600 µg*
Elution Volume	1-2 mL
Analyte Purified	Genomic DNA, mitochondrial DNA, viral DNA
Time to Complete 10 Purifications	50-65 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

You have selected: Cat. 46350

Kit Specifications
Maximum Blood Input	200 μL
Column Binding Capacity	> 50 μL
Average Yield (200 μL of blood)	2-8 μg*
Time to Complete 96 Purifications	45 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

You have selected: Cat. 59800

Kit Specifications
Maximum Blood Input	200 µL
Average Yield (200 µL blood)*	4-12 µg
Average Purity (OD260/280)	1.8 - 1.9
Time to Complete Purifications	40 minutes (hands-on time Cat. 59800) 50 minutes (hands-on time Cat. 62600)

* Average DNA yield will vary depending on the donor

You have selected: Cat. 62600

Kit Specifications
Maximum Blood Input	200 µL
Average Yield (200 µL blood)*	4-12 µg
Average Purity (OD260/280)	1.8 - 1.9
Time to Complete Purifications	40 minutes (hands-on time Cat. 59800) 50 minutes (hands-on time Cat. 62600)

* Average DNA yield will vary depending on the donor

Supporting Data

Figure 1. The difference of Ct values obtained from a Taqman® qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants. 3 and 9 μL of each solution were used in a 20 μL qPCR reaction involving GAPDH primers.

Figure 2. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit. 15 μL of 200 μL elutions were run on 1X TAE 1.0% agarose gel. Marker= Norgen's UltraRanger DNA Ladder.

Figure 3. The difference in Ct values between DNA isolated using Norgen's Blood Genomic DNA Isolation Mini Kit (Norgen), Competitor A's DNA Blood Mini Kit (A), Competitor B's Blood Kit (B), and Competitor B's Dx Blood (B Dx), in a Taqman qPCR reaction. Different template volumes (1, 3, 6 & 9 μL) of each elution were used in a 20 μL qPCR reaction involving GAPDH primers.

Figure 5. High Yields of DNA Isolated from Whole Blood. DNA was isolated from two different 10mL whole blood samples using Norgen's Blood DNA Isolation Maxi Kit. Following isolation, 10 µL from each 1 mL elution was loaded on 1% TAE agarose gel. Lanes 1 and 3 correspond to the first elution, while Lanes 2 and 4 correspond to the second elution. Norgen's Blood DNA Isolation Maxi Kit demonstrated a high yield of intact DNA.

Figure 6. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 10 mL of whole human blood using Norgen's Blood DNA Isolation Maxi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 1mL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 4. The difference in yield isolated from different volumes of blood using Norgen's vs Qiagen's kit. 50 μL of each sample was diluted in 450 μL of nuclease-free water, and DNA concentrations were measured using the UltraSpec 2100 Pro (Fisher Scientific). Two elutions were performed for all samples.

Figure 5. The difference of Ct values obtained from a Taqman® qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants. 3 and 9 μL of each solution were used in a 20 μL qPCR reaction involving GAPDH primers.

Figure 6. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit. 15 μL of 200 μL elutions were run on 1X TAE 1.0% agarose gel. Marker= Norgen's UltraRanger DNA Ladder.

Figure 7. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood. DNA was isolated from 20, 50, 100 and 200 µL of whole blood using Norgen's Blood DNA Isolation Mini Kit and a leading competitor's kit. Following isolation, 15 µL from each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Mini Kit demonstrated a better DNA yield than the leading competitor's kit. Lane M: Norgens UltraRanger 1kb DNA Ladder.

Figure 8. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood DNA Isolation Mini Kit (Blue) and a leading competitor's kit (Red). 9 µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value, indicating the higher yield and purity of DNA isolated using Norgen's kit. The black line is a no-template control.

Figure 9. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood DNA Isolation Mini Kit (Blue) and a leading competitor's kit (Red). Nine µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, with a linear decrease in Ct value with the increase in blood input volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value from all DNA isolated from the different blood input volumes, indicating the higher yield and purity of DNA isolated using Norgen's kit.

Figure 10. High Yields of DNA Isolated from 2 mL of Whole Blood. DNA was isolated from 2 mL of whole blood using Norgen's Blood DNA Isolation Midi Kit. Following isolation, 20 µL from 300 µL first and second elutions was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Midi Kit demonstrated a high DNA yield and integrity. Lane L: Norgen's HighRanger 1kb DNA Ladder.

Figure 11. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 2 mL of whole human blood using Norgen's Blood DNA Isolation Midi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 300 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 12. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 2 mL of whole human blood using Norgen's Blood DNA Isolation Midi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 300 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene with a linear decrease in Ct value with the increased DNA template volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.

Figure 13. High Yields of DNA Isolated from 200 µL of Whole Blood. DNA was isolated from 200 µL of whole blood using Norgen's Blood DNA Isolation 96-Well Kit. Following isolation of 12 samples, 15 µL from each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation 96-Well Kit demonstrated a good and consistent DNA yield and integrity. Lane M: Norgen's HighRanger 1kb DNA Ladder.

Figure 14. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 200 µL of whole human blood using Norgen's Blood DNA Isolation 96-Well Kit. Five µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene from all the isolated 12 samples (blue). This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 15. DNA Isolated from Blood Preserved in 3 Different Anticoagulants. DNA was isolated from 200 µL of human whole blood samples preserved in three different anticoagulants (Heparin: H, EDTA: E and Na Citrate: C) using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). For evaluation, 10 µL from each 200 µL of elution were run on 1X TAE 1.2% agarose gel. Norgen's Blood DNA Isolation Kit (Magnetic Bead System) showed an intact and comparable DNA profile to the column method from all three different anticoagulant mixed blood samples. Marker = Norgen's HighRanger DNA Ladder.

Figure 16. High Yield of Blood DNA. DNA was isolated from 200 µL of human whole blood samples preserved in three different anticoagulants (Heparin, EDTA and Na Citrate) using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). The total DNA yield from all the elutions was compared, and it was found that the Magnetic Bead System isolated a similar DNA yield to the column method from all the different anticoagulants tested.

Figure 17. Detection of GAPDH from blood samples preserved in three different anticoagulants (Heparin: red, EDTA: green and Na Citrate: blue). DNA was isolated from 200 µL human whole blood samples preserved in three different anticoagulants using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL (circle), 4 µL (triangle) and 8 µL (cross) (total PCR reaction volume was 20 µL). No PCR inhibition was observed up to 8 µL of PCR template, indicating the excellent quality of blood DNA isolated using Norgens Blood DNA Isolation Kit (Magnetic Bead System) similar to the column method (Cat. 46300).

Click for expanded view

Protocols:

(31200) Blood DNA Isolation Maxi Kit - Protocol (12 prep)

(46300) Blood DNA Isolation Mini Kit - Protocol (50 prep)

(46380) Blood DNA Isolation Mini Kit - Protocol (100 prep)

(46350) Blood DNA Isolation 96-Well Kit (High Throughput) - Protocol (2 x 96-well)

(51400) Blood DNA Isolation Midi Kit - Protocol (20 prep)

(59800) Blood DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep)

(62600) Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)

Application Notes:

Blood DNA Isolation Maxi Kit Genomic DNA Isolation From Blood Collected On Different Anticoagulants Citrate Edta Heparin - Application Note

Genomic DNA Isolation from 1 µL – 100 µL of Whole Blood using Norgen’s Blood Genomic DNA Isolation Micro Kit

Effect of Elution Volume on DNA Recovery and Quality using Norgen’s Blood Genomic DNA Isolation Micro Kit

Single Elution vs. Re-Elution: Implications on Concentration and Purity using the Blood Genomic DNA Isolation Micro Kit

Product Flyers:

Blood DNA Isolation Kit

Title	Lack of age-related mosaic loss of W chromosome in long-lived birds
Journal	Biology Letters.2019.
Authors	Nancy Trujillo, Mónica Martínez-Pacheco, Cecilia Soldatini, Sergio Ancona, Rebecca C. Young, Yuri V. Albores-Barajas, Alberto H. Orta, Cristina Rodríguez, Tamas Székely, Hugh Drummond, Araxi O. Urrutia and Diego Cortez

Title	Associations of + 138 Ins/del A and + 5665 G/T polymorphisms of endothelin-1 gene with hypertension in Burmese people in Magway, Myanmar
Journal	Clinical Hypertension.2019.
Authors	Win Min Oo, Kyaw Thiha & Myat Mon Khine

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Blood DNA Isolation Kits

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 46300

Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

You have selected: Cat. 46380

Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

You have selected: Cat. 51400

Kit Specifications
Minimum Blood Input	0.3 mL
Maximum Blood Input	2 mL
Column Binding Capacity	> 100 µg
Average Yield	60 µg*
Elution Volume	200-300 uL
Analyte Purified	Genomic DNA, mitochondrial DNA, viral DNA, bacterial DNA
Time to Complete 10 Purifications	50-60 minutes

*Yield will vary depending on the type of blood processed

You have selected: Cat. 31200

Kit Specifications
Maximum Blood Input	10 mL
Column Binding Capacity	> 500 µg
Average Yield	200-600 µg*
Elution Volume	1-2 mL
Analyte Purified	Genomic DNA, mitochondrial DNA, viral DNA
Time to Complete 10 Purifications	50-65 minutes

*Yield will vary depending on the type of blood processed

You have selected: Cat. 46350

Kit Specifications
Maximum Blood Input	200 μL
Column Binding Capacity	> 50 μL
Average Yield (200 μL of blood)	2-8 μg*
Time to Complete 96 Purifications	45 minutes

You have selected: Cat. 59800

Kit Specifications
Maximum Blood Input	200 µL
Average Yield (200 µL blood)*	4-12 µg
Average Purity (OD260/280)	1.8 - 1.9
Time to Complete Purifications	40 minutes (hands-on time Cat. 59800) 50 minutes (hands-on time Cat. 62600)

* Average DNA yield will vary depending on the donor

You have selected: Cat. 62600

Kit Specifications
Maximum Blood Input	200 µL
Average Yield (200 µL blood)*	4-12 µg
Average Purity (OD260/280)	1.8 - 1.9
Time to Complete Purifications	40 minutes (hands-on time Cat. 59800) 50 minutes (hands-on time Cat. 62600)

* Average DNA yield will vary depending on the donor

Protocols:

(31200) Blood DNA Isolation Maxi Kit - Protocol (12 prep)

(46300) Blood DNA Isolation Mini Kit - Protocol (50 prep)

(46380) Blood DNA Isolation Mini Kit - Protocol (100 prep)

(46350) Blood DNA Isolation 96-Well Kit (High Throughput) - Protocol (2 x 96-well)

(51400) Blood DNA Isolation Midi Kit - Protocol (20 prep)

(59800) Blood DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep)

(62600) Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)

Application Notes:

Blood DNA Isolation Maxi Kit Genomic DNA Isolation From Blood Collected On Different Anticoagulants Citrate Edta Heparin - Application Note

Genomic DNA Isolation from 1 µL – 100 µL of Whole Blood using Norgen’s Blood Genomic DNA Isolation Micro Kit

Effect of Elution Volume on DNA Recovery and Quality using Norgen’s Blood Genomic DNA Isolation Micro Kit

Single Elution vs. Re-Elution: Implications on Concentration and Purity using the Blood Genomic DNA Isolation Micro Kit

Product Flyers:

Blood DNA Isolation Kit

Title	Lack of age-related mosaic loss of W chromosome in long-lived birds
Journal	Biology Letters.2019.
Authors	Nancy Trujillo, Mónica Martínez-Pacheco, Cecilia Soldatini, Sergio Ancona, Rebecca C. Young, Yuri V. Albores-Barajas, A

Improve Your Workflow with Best-in-Class Total RNA Purification Technology | Learn More

Blood DNA Isolation Kits (Cat. 46300, 46380, 51400, 31200, 46350, 59800, 62600)

Blood DNA Isolation Kits

Blood DNA Isolation Kits

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Request a Product Sample

Blood DNA Isolation Kits (Cat. 46300, 46380, 51400, 31200, 46350, 59800, 62600)

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Blood DNA Isolation Kits

Blood DNA Isolation Kits

Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Request a Product Sample

Product Overview

Documentation

Citations

Receive Complimentary
NUCLEASE-FREE WATER

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.