Dried Blood Spot (DBS) DNA Isolation Kit

Rapid preparation of total DNA from dried blood spots

For research use only and NOT intended for in vitro diagnostics.

Norgen's DBS DNA Isolation Kit is a seamless alternative for Norgen's discontinued Blood DNA kit (52100).

Dried Blood Spot (DBS) DNA Isolation Kit

SKU

36000

Rapid preparation of total DNA from dried blood spots

$174.00

SKU

36000

Format

Size

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Features and Benefits

Optimized to isolate total genomic DNA from dried blood on collection cards making it suitable for newborn screening cards and other DBS formats.
Available in the following formats:
- spin column: simpler and faster than traditional organic extraction or precipitation techniques.
- magnetic bead system: replaces centrifuge steps and requires minimal laboratory equipment.
Capable of isolating DNA from 2 - 6 punches of 3 mm diameter.
The purified DNA is free from RNA contamination and suitable for most molecular biology applications.
Typical processing time is ~30–35 min for spin column, and 40 min for magnetic bead system, enabling quicker workflows in research labs
Applicable to blood samples from humans and other species, increasing versatility in comparative studies.

Dried Blood Spot DNA Isolation Kit (Spin Column)

This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column. The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes. The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.

Dried Blood Spot DNA Isolation Kit (Magnetic Bead System)

Designed for the rapid preparation of genomic DNA from up to 6 x 3 mm (1/8 inch) diameter circles. Purification is based on Magnetic bead as the separation matrix. Norgen’s Magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Details

Supporting Data

Figure 1. Linear and better DNA yield from 2-6 DBS punches. DBS cards were prepared using an equal volume of EDTA blood from the same sample, ensuring uniform saturation. The cards were allowed to air dry overnight at room temperature. Multiple 3 mm circles were punched out for DNA isolation. Either 2, 4, or 6 punches were processed for DNA isolation using Norgen’s DBS DNA isolation kit or Competitor Q kit, following the respective manufacturer’s protocols. DNA yield from Norgen’s kit was higher and more linear across all input sizes.

Figure 2. DNA Isolated from 3 x 3 mm Diameter Circles. Blood collected on EDTA was applied to Whatman's 903 Protein Saver Card and allowed to dry for 1 week. DNA was isolated from 3 x 3 mm diameter circles per sample using Norgen's Dried Blood spot DNA Isolation Kit. Following isolation, 15 µL from each 150 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Kit demonstrated a good DNA yield and integrity. The ladder corresponds to Norgen's UltraRanger 1kb DNA Ladder.

Figure 3. Purified DNA can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 3 x 3 mm diameter circles per sample using Norgen's Dried Blood Spot DNA Isolation Kit. Next, 3 µL (green line) & 9 µL (blue line) of the DNA from each of the 150 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Kit Specifications
Maximum Blood Input	Up to 6 x 3 mm (1/8 inch) diameter punches
Average yield* (ng)	150 ng
Column Binding Capacity	> 50 µg
Time to Complete 10 Purifications	35 minutes

* Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Component	Cat. 36000 (50 preps)	Cat. 73600 (50 preps)
Lysis Buffer B	20 mL	20 mL
Wash Solution WN	18 mL	18 mL
Wash Solution A	18 mL	-
Magnetic Beads A	-	2.2 mL
Elution Buffer B	15 mL	8 mL
Proteinase K in Storage Buffer	1.2 mL	1.2 mL
Spin Columns	50	-
Collection Tubes	50	-
Elution Tubes	50	50 (2 x 25)
Product Insert	1	1

FAQs

I lost / finished the proteinase K in storage buffer from my Norgen Biotek kit. Can I purchase it separately?

Yes, you can purchase more Proteinase K, and it is supplied in a pack size of 5 x 1 mL tubes. You can purchase here, or contact our technical support team at support@norgenbiotek.com and request for Product number 28229.

I noticed the column was clogged. What causes this?

Column clogging can result from the following:

Inefficient cell lysis.
Check Proteinase K activity. Also ensure that the correct volume of Lysis Buffer B was added to the blood sample.
Cell debris may be clogging the column.
When a high cell number is expected in the blood sample, ensure that the optional spin for 2 minutes at 5,000 rpm after the Proteinase K incubation is performed. Take the clean supernatant only for the next binding step.
The sample is too large.
Too many cells were applied to the column. Ensure that Proteinase K and Lysis Buffer B are proportionally added as the blood volume is increased. Clogging can be alleviated by centrifuging for a longer period of time until the lysate passes through the column.

Why do I have a low genomic DNA yield?

A low genomic DNA yield may be caused by inefficient cell lysis. Ensure that the correct volume of Lysis Buffer B was added to the blood sample. Also increase incubation time up to 25-35 minutes at 56°C. Additionally, ensure that the correct volume of ethanol was added to the lysate before column binding.

Why is the purified DNA not performing well in downstream applications?

If the DNA does not perform well in downstream applications, It may be due to one or more of the following:

DNA was not washed three times with the provided solutions.
Ensure the column was washed one time with Solution WN and two times with Wash Solution A.
Ethanol carryover.
Ensure that the dry spin under the Column Wash procedure is performed, in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications.
High DNA input used in PCR reaction.
For best results, make sure that the final concentration of DNA in the PCR reaction does not exceed 75 ng/μL (1.5 μg DNA per 20 μL PCR reaction)

Title	Genetic polymorphisms of CYP2C19 in ecuadorian population: An interethnic approach
Citation	Heliyon 2024.
Authors	Alba Alonso Llorente a,b,c,* , Josefa Salgado Garrido d,e , Oscar ´ Teijido Hermida f, Fabricio Gonzalez ´ Andrade g , Alberto Valiente Martín d , Ana Julia Fanlo Villacampa b, Jorge Vicente Romero

Title	The Correlation of Two Different Real-Time PCR Devices for the Analysis of CYP2C19 Pharmacogenetic Results
Citation	Scientia Pharmaceutica 2023.
Authors	Alonso Llorente, Alba, Josefa Salgado Garrido, Oscar Teijido Hermida, Fabricio González Andrade, Alberto Valiente Martín, Ana Fanlo Villacampa, and Jorge Vicente Romero.

Title	Evaluation of Dried Blood Spot Samples for Hepatitis C Virus Detection and Quantification
Citation	Journal of Clinical Virology 2016.
Authors	Marques, B. L. C., do Espírito-Santo, M. P., Marques, V. A., Miguel, J. C., da Silva, E. F., Villela-Nogueira, C. A., ... & Villar, L. M

Title	Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting
Citation	Malaria Journal 2016.
Authors	Simona Gabrielli1 , Livia Bellina2 , Giovanni Luigi Milardi1 , Boniface Kabasele Katende3 , Valentina Totino1 , Valerio Fullin3 and Gabriella Cancrini

Dried Blood Spot (DBS) DNA Isolation Kit

Features and Benefits

Dried Blood Spot DNA Isolation Kit (Spin Column)

Dried Blood Spot DNA Isolation Kit (Magnetic Bead System)

Details

Supporting Data

Documentation

PROTOCOLS

APPLICATION NOTES

SAFETY DATA SHEETS

FAQs

I lost / finished the proteinase K in storage buffer from my Norgen Biotek kit. Can I purchase it separately?

I noticed the column was clogged. What causes this?

Why do I have a low genomic DNA yield?

Why is the purified DNA not performing well in downstream applications?

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Dried Blood Spot (DBS) DNA Isolation Kit

Dried Blood Spot (DBS) DNA Isolation Kit

Features and Benefits

Dried Blood Spot DNA Isolation Kit (Spin Column)

Dried Blood Spot DNA Isolation Kit (Magnetic Bead System)

Details

Supporting Data

Documentation

FAQs

Citations

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