Blood Genomic DNA Isolation Mini Kit Dx (Cat. Dx46300)
Blood Genomic DNA Isolation Mini Kit Dx
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Blood Genomic DNA Isolation Mini Kit Dx
For the rapid preparation of high quality DNA from whole blood for in vitro diagnostic use
- CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
- Input volumes up to 200 µL
- High yield and high quality DNA ready for any application
- Fast and convenient spin column protocol
Note: This product is not available for sale in the United States
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(Cat. Dx46300) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
| Minimum Blood Input | 20 µL |
|
Maximum Blood Input
|
200 µL
|
|
Column Binding Capacity
|
> 50 µg
|
|
Average Yield (200 µL of blood)
|
4-12 µg*
|
|
Time to Complete 10 Purifications
|
30 minutes
|
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.
Supporting Data
| Component | Cat. Dx46300 (50 preps) |
|---|---|
| Lysis Solution | 20 mL |
| Wash Solution I | 18 mL |
| Wash Solution II | 18 mL |
| Elution Buffer | 12 mL |
| Proteinase K | 1.2 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes | 50 |
| Product Insert | 1 |
| Title | Prenatal Diagnosis Using Chromosomal Microarray Analysis in High-Risk Pregnancies |
| Journal | J. Clin. Med. 2022. |
| Authors | Ching-Hua Hsiao, Jia-Shing Chen, Yu-Ming Shiao, Yann-Jang Chen, Ching-Hsuan Chen, Woei-Chyn Chu and Yi-Cheng Wu |
| Title | Amendment of amino acid in Q192R genetic polymorphism of paraoxonase 1 is a conventional risk factor for type 2 diabetes mellitus in the Saudi population |
| Journal | Int J Clin Exp Med. 2016. |
| Authors | Khalid Khalaf Alharbi, Fawiziah Khalaf Alharbi, Fahad Khalaf Alharbi, Hazem K Ghneim, A.M. Al-Sulaiman, Abdulaziz A. Alodhayani, Shaik Nazia Tabassum, Imran Ali Khan |
| Title | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
| Journal | Asian Pacific Journal of Reproduction. 2016. |
| Authors | Afifi Inayah, Sri Rahayu, Nashi Widodo, Widya Ayu Prasdini |
| Title | Screening for genetic mutations in LDLR gene with familial hypercholesterolemia patients in the Saudi population |
| Journal | Acta Biochimica Polonica. 2015. |
| Authors | Alharbi KK, Kashour TS, Al-Hussaini W, Nbaheen MS, Hasanato RM, Mohamed S, Tamimi W, Khan IA |
| Title | Genetic polymorphisms in paraoxonase 1 and G protein-coupled receptor 77, and the risk of glucose-6-phosphate dehydrogenase deficiency in a Saudi population. |
| Journal | Saudi Medical Journal. 2015. |
| Authors | Khalid K. Alharbi |
| Title | Prevalence of glucose-6-phosphate dehydrogenase deficiency and the role of the A− variant in a Saudi population. |
| Journal | Journal of International Medical Research. 2014. |
| Authors | Khalid Khalaf Alharbi and Imran Ali Khan |
| Title | Polymorphic variants of MnSOD Val16Ala, CAT-262 C < T and GPx1 Pro198Leu genotypes and the risk of laryngeal cancer in a smoking population. |
| Journal | The Journal Laryngology and Otology. 2013. |
| Authors | Aynali G, Doğan M, Sütcü R, Yüksel O, Yariktaş M, Unal F, Yasan H, Ceyhan B, Tüz M. |
| Title | Genetic differentiation and inheritance of random amplified polymorphic DNA (RAPD) markers in pectoral spine phenotypic sub-groups of Clarias gariepinus. |
| Journal | African Journal of Biotechnology. 2013. |
| Authors | Oyebola O, Omitoyin B, Salako A, Ajani E, Awodiran M |
| Title | Non Genetic Risk Factor and Polymorphism of Aldosterone Synthase T(-344)C Variant CYP11B2 Gene Promoter Region Essential Hypertension Patients at Coastal and Mountainous Areas. |
| Journal | Jurnal Kedokteran Brawijaya. 2013. |
| Authors | Sundari, Aulani'am, Wahono D, Widodo M. |
| Title | Thrombophilic gene polymorphism studies in G6PD deficient individuals from Saudi population. |
| Journal | Bioinformation. 2012. |
| Authors | Alharbi KK, Khan IA, Syed R. |
Blood Genomic DNA Isolation Mini Kit Dx
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood for subsequent in vitro diagnostic use. Both fresh and frozen anticoagulated blood may be used with this procedure. Purification is based on spin column chromatography as the separation matrix. Norgen’s column binds DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification. Any diagnostic results generated using the DNA isolated with Norgen’s Blood Genomic DNA Isolation Mini Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including experience with whole blood samples and DNA isolation.
Norgen’s Blood Genomic DNA Isolation Mini Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
| Minimum Blood Input | 20 µL |
|
Maximum Blood Input
|
200 µL
|
|
Column Binding Capacity
|
> 50 µg
|
|
Average Yield (200 µL of blood)
|
4-12 µg*
|
|
Time to Complete 10 Purifications
|
30 minutes
|
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.
| Component | Cat. Dx46300 (50 preps) |
|---|---|
| Lysis Solution | 20 mL |
| Wash Solution I | 18 mL |
| Wash Solution II | 18 mL |
| Elution Buffer | 12 mL |
| Proteinase K | 1.2 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes | 50 |
| Product Insert | 1 |
| Title | Prenatal Diagnosis Using Chromosomal Microarray Analysis in High-Risk Pregnancies |
| Journal | J. Clin. Med. 2022. |
| Authors | Ching-Hua Hsiao, Jia-Shing Chen, Yu-Ming Shiao, Yann-Jang Chen, Ching-Hsuan Chen, Woei-Chyn Chu and Yi-Cheng Wu |
| Title | Amendment of amino acid in Q192R genetic polymorphism of paraoxonase 1 is a conventional risk factor for type 2 diabetes mellitus in the Saudi population |
| Journal | Int J Clin Exp Med. 2016. |
| Authors | Khalid Khalaf Alharbi, Fawiziah Khalaf Alharbi, Fahad Khalaf Alharbi, Hazem K Ghneim, A.M. Al-Sulaiman, Abdulaziz A. Alodhayani, Shaik Nazia Tabassum, Imran Ali Khan |
| Title | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
| Journal | Asian Pacific Journal of Reproduction. 2016. |
| Authors | Afifi Inayah, Sri Rahayu, Nashi Widodo, Widya Ayu Prasdini |
| Title | Screening for genetic mutations in LDLR gene with familial hypercholesterolemia patients in the Saudi population |
| Journal | Acta Biochimica Polonica. 2015. |
| Authors | Alharbi KK, Kashour TS, Al-Hussaini W, Nbaheen MS, Hasanato RM, Mohamed S, Tamimi W, Khan IA |
| Title | Genetic polymorphisms in paraoxonase 1 and G protein-coupled receptor 77, and the risk of glucose-6-phosphate dehydrogenase deficiency in a Saudi population. |
| Journal | Saudi Medical Journal. 2015. |
| Authors | Khalid K. Alharbi |
| Title | Prevalence of glucose-6-phosphate dehydrogenase deficiency and the role of the A− variant in a Saudi population. |
| Journal | Journal of International Medical Research. 2014. |
| Authors | Khalid Khalaf Alharbi and Imran Ali Khan |
| Title | Polymorphic variants of MnSOD Val16Ala, CAT-262 C < T and GPx1 Pro198Leu genotypes and the risk of laryngeal cancer in a smoking population. |
| Journal | The Journal Laryngology and Otology. 2013. |
| Authors | Aynali G, Doğan M, Sütcü R, Yüksel O, Yariktaş M, Unal F, Yasan H, Ceyhan B, Tüz M. |
| Title | Genetic differentiation and inheritance of random amplified polymorphic DNA (RAPD) markers in pectoral spine phenotypic sub-groups of Clarias gariepinus. |
| Journal | African Journal of Biotechnology. 2013. |
| Authors | Oyebola O, Omitoyin B, Salako A, Ajani E, Awodiran M |
| Title | Non Genetic Risk Factor and Polymorphism of Aldosterone Synthase T(-344)C Variant CYP11B2 Gene Promoter Region Essential Hypertension Patients at Coastal and Mountainous Areas. |
| Journal | Jurnal Kedokteran Brawijaya. 2013. |
| Authors | Sundari, Aulani'am, Wahono D, Widodo M. |
| Title | Thrombophilic gene polymorphism studies in G6PD deficient individuals from Saudi population. |
| Journal | Bioinformation. 2012. |
| Authors | Alharbi KK, Khan IA, Syed R. |
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