Blood Genomic DNA Isolation Mini Kit Dx For the rapid preparation of high quality DNA from whole blood for in vitro diagnostic use Note: This product is not available for sale in the United States Powered by Bioz Blood Genomic DNA Isolation Mini Kit Dx For the rapid preparation of high quality DNA from whole blood for in vitro diagnostic use Price USD$175.00 SKU Dx46300 Format Mini Size 50 Preps Quantity Overview Details Documentation Citations Overview CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746. Input volumes up to 200 µL High yield and high quality DNA ready for any application Fast and convenient spin column protocol Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood for subsequent in vitro diagnostic use. Both fresh and frozen anticoagulated blood may be used with this procedure. Purification is based on spin column chromatography as the separation matrix. Norgen’s column binds DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification. Any diagnostic results generated using the DNA isolated with Norgen’s Blood Genomic DNA Isolation Mini Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including experience with whole blood samples and DNA isolation. Norgen’s Blood Genomic DNA Isolation Mini Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay. Details Supporting Data Previous Figure 1. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood. DNA was isolated from 20, 50, 100 and 200 µL of whole blood using Norgen's Blood Genomic DNA Isolation Mini Kit Dx and a leading competitor's kit. Following isolation, 15 µL from each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Mini Kit Dx demonstrated a better DNA yield than the leading competitor's kit. Lane M: Norgen’s UltraRanger 1kb DNA Ladder. Figure 2. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction.DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood Genomic DNA Isolation Mini Kit Dx (Blue) and a leading competitor's kit (Red). Nine µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value, indicating the higher yield and purity of DNA isolated using Norgen's kit. The black line is a no-template control. Figure 3. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood Genomic DNA Isolation Mini Kit Dx (Blue) and a leading competitor's kit (Red). Nine µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, with a linear decrease in Ct value with the increase in blood input volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value from all DNA isolated from the different blood input volumes, indicating the higher yield and purity of DNA isolated using Norgen's kit. Next Click for expanded view Kit Specifications Minimum Blood Input 20 µL Maximum Blood Input 200 µL Column Binding Capacity > 50 µg Average Yield (200 µL of blood) 4-12 µg* Time to Complete 10 Purifications 30 minutes *Yield will vary depending on the type of blood processed Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended. Component Cat. Dx46300 (50 preps) Lysis Solution 20 mL Wash Solution I 18 mL Wash Solution II 18 mL Elution Buffer 12 mL Proteinase K 1.2 mL Spin Columns 50 Collection Tubes 50 Elution Tubes 50 Product Insert 1 Documentation PROTOCOLS (Dx46300) Blood Genomic DNA Isolation Mini Kit Dx - Protocol (50 prep) PRODUCT INFORMATION SHEETS Blood DNA Isolation Mini Kit - Product Information Sheet APPLICATION NOTES Comparative Study of DNA Isolated from Increasing Volumes of Blood Using Norgen’s Blood Genomic DNA Isolation Kit Versus Qiagen’ Comparative Study of DNA Isolated from Whole Blood Using Norgen’s Blood Genomic DNA Isolation Mini Kit and Competitors’ Kits Genomic DNA Isolation from Buffy Coat using Norgen’s Blood Genomic DNA Isolation Mini Kit Genomic DNA Isolation from blood collected on different anticoagulants: Citrate, EDTA & Heparin Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device SAFETY DATA SHEETS 46300, 46380 - Safety Data Sheet POSTERS Blood DNA Isolation Mini Kit Sensitivity of DNA Extraction Methods From Different Bodily Fluids For Human Identification - Poster FLYERS Flyer Citations Powered by Bioz See more details on Bioz Related Products Dried Blood Spot (DBS) DNA Isolation Kit Leukocyte RNA Purification Kit
Blood Genomic DNA Isolation Mini Kit Dx For the rapid preparation of high quality DNA from whole blood for in vitro diagnostic use Note: This product is not available for sale in the United States Powered by Bioz Blood Genomic DNA Isolation Mini Kit Dx For the rapid preparation of high quality DNA from whole blood for in vitro diagnostic use Price USD$175.00 SKU Dx46300 Format Mini Size 50 Preps Quantity