Figure 2. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction.DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood Genomic DNA Isolation Mini Kit Dx (Blue) and a leading competitor's kit (Red). Nine µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value, indicating the higher yield and purity of DNA isolated using Norgen's kit. The black line is a no-template control.

Figure 3. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood Genomic DNA Isolation Mini Kit Dx (Blue) and a leading competitor's kit (Red). Nine µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, with a linear decrease in Ct value with the increase in blood input volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value from all DNA isolated from the different blood input volumes, indicating the higher yield and purity of DNA isolated using Norgen's kit.