Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Detection Kits

Features and Benefits

Detection kits for CCCVd
Available in TaqMan format for analysis

Cadang-cadang is a lethal disease of coconut (Cocos nucifera) caused by the Coconut cadangcadang viroid (CCCVd). The coconut cadang-cadang viroid is the smallest known pathogen and is biologically distinct from other viroids. The viroid consists of circular or linear single-stranded RNA with a size of 246 or 247 nucleotides. The viroid is mechanically transmissible via nucleic acid inocula, however the mode of natural transmission is not known. The viroid is only detected in Palmae. The mode of natural transmission is unknown and eradication measures fail to control the disease. Norgen’s Coconut Cadang-Cadang Viroid End-Point RT-PCR Detection Kit is a rapid and sensitive CCCVd detection tool which can be applicable for the early diagnosis of CCCVd.

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CCCVd TaqMan RT-PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

CCCVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM47950 (100 preps)	Cat. TM47910 (100 preps)
MDx TaqMan 2X PCR Master Mix	3 x 350 μL	-
CCCVd Primer & Probe Mix	3 x 70 μL	280 μL
CCCVd Positive Control	3 x 50 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1