Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits (Cat. TM47950, TM47910)

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Cat. TM47950, TM47910

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Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Cat. TM47950, TM47910

Detection kits for CCCVd
Available in TaqMan format for analysis

For research use only and NOT intended for in vitro diagnostics.

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CCCVd TaqMan RT-PCR Kit
(Cat. TM47950) 100 rxns

CCCVd TaqMan Probe/Primer and Control Set
(Cat. TM47910) 100 rxns

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. TM47950

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

You have selected: Cat. TM47910

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqManPCR Kit-Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

Click for expanded view

Component	Cat. TM47950 (100 preps)	Cat. TM47910 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
CCCVd Primer & Probe Mix	280 μL	280 μL
CCCVd Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Protocols:

(TM47950) Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Kit - Protocol (100 rxns)

Product Overview
Documentation

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Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Cadang-cadang is a lethal disease of coconut (Cocos nucifera) caused by the Coconut cadangcadang viroid (CCCVd). The coconut cadang-cadang viroid is the smallest known pathogen and is biologically distinct from other viroids. The viroid consists of circular or linear single-stranded RNA with a size of 246 or 247 nucleotides. The viroid is mechanically transmissible via nucleic acid inocula, however the mode of natural transmission is not known. The viroid is only detected in Palmae. The mode of natural transmission is unknown and eradication measures fail to control the disease. Norgen’s Coconut Cadang-Cadang Viroid End-Point RT-PCR Detection Kit is a rapid and sensitive CCCVd detection tool which can be applicable for the early diagnosis of CCCVd.

Click to expand options

CCCVd TaqMan RT-PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

CCCVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqManPCR Kit-Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

Click for expanded view

Kit Specifications

You have selected: Cat. TM47950

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

You have selected: Cat. TM47910

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM47950 (100 preps)	Cat. TM47910 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
CCCVd Primer & Probe Mix	280 μL	280 μL
CCCVd Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Protocols:

(TM47950) Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Kit - Protocol (100 rxns)

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits (Cat. TM47950, TM47910)

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Related Products

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Plant RNA/DNA Purification Kit

Plant/Fungi Total RNA Purification Kit

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits (Cat. TM47950, TM47910)

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Product Overview

Documentation

Related Products

Coconut cadangcadang viroid (CCCVd) TaqMan RT-PCR Detection Kits

Plant RNA/DNA Purification Kit

Plant/Fungi Total RNA Purification Kit

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.