Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Detection Kits Price USD$475.00 SKU TM47910 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for CCCVd Available in TaqMan format for analysis Cadang-cadang is a lethal disease of coconut (Cocos nucifera) caused by the Coconut cadangcadang viroid (CCCVd). The coconut cadang-cadang viroid is the smallest known pathogen and is biologically distinct from other viroids. The viroid consists of circular or linear single-stranded RNA with a size of 246 or 247 nucleotides. The viroid is mechanically transmissible via nucleic acid inocula, however the mode of natural transmission is not known. The viroid is only detected in Palmae. The mode of natural transmission is unknown and eradication measures fail to control the disease. Norgen’s Coconut Cadang-Cadang Viroid End-Point RT-PCR Detection Kit is a rapid and sensitive CCCVd detection tool which can be applicable for the early diagnosis of CCCVd. TaqMan PCR Kit Click to expand options CCCVd TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents CCCVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Component Cat. TM47950 (100 preps) Cat. TM47910 (100 preps) MDx TaqMan 2X PCR Master Mix 3 x 350 μL - CCCVd Primer & Probe Mix 3 x 70 μL 280 μL CCCVd Positive Control 3 x 50 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM47950) Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Kit - Protocol (100 rxns) Related Products Plant RNA/DNA Purification Kit Plant/Fungi Total RNA Purification Kit Coconut Cadang-Cadang Viroid (CCCVd) TaqMan Probe/Primer and Control Set
Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Coconut Cadang-Cadang Viroid (CCCVd) TaqMan RT-PCR Detection Kits Price USD$475.00 SKU TM47910 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
