Cell Culture Media Exosome Purification and RNA Isolation Kits

Cat. 60700, 60800, 60900
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Cell Culture Media Exosome Purification and RNA Isolation Kits

Cat. 60700, 60800, 60900

All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volumes
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Cell Culture Media Exosome Purification and RNA Isolation Mini Kit
(Cat. 60700)
50 preps
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit
(Cat. 60800)
25 preps
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
(Cat. 60900)
15 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volumes
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Cell Culture Media Exosome Purification and RNA Isolation Kits

Norgen's Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

For sample input volumes ranging from 5 mL to 10 mL.

Cell Culture Media Exosome Purification and RNA Isolation Midi Kit

For sample input volumes ranging from 10 mL to 20 mL.

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

For sample input volumes ranging from 20 mL to 35 mL.

Kit Specifications

You have selected: Cat. 60700
Kit Specifications
Sample Type
Cell-Free Cell Culture Media
Sample Volume Range
5 mL to 10 mL
Size of RNA Purified
All sizes, including miRNA and small RNA (<200 nt) 
Elution Volume
50-100 µL
Time to Complete 10 Purifications
35 to 40 minutes
Average Yields*
Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 60800
Kit Specifications
Sample Type
Cell-Free Cell Culture Media
Sample Volume Range
10 mL to 20 mL
Size of RNA Purified
All sizes, including miRNA
and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields*
Variable depending on specimen

*Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60ºC if any salt precipitation is observed.

You have selected: Cat. 60900
Kit Specifications
Sample Type Cell-Free Cell Culture Media
Sample Volume Range 20 mL to 35 mL
Size of RNA Purified All sizes, including small RNA
(<200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications 40 to 45 minutes
Average Yields* Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Supporting Data

Click for expanded view

Component Cat. 60700 (50 preps) Cat. 60800 (25 preps) Cat. 60900 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 1.5 mL 1.5 mL 1.5 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Column 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution tubes (1.7 mL) 100 50 30
Product Insert 1 1 1

Mini (60700)

Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Midi (60800)

Title Small RNA-sequence analysis of plasma-derived extracellular vesicle miRNAs in smokers and patients with chronic obstructive pulmonary disease as circulating biomarkers
Journal Journal of Extracellular Vesicles, 8(1). 2019.
Authors Sundar, I. K., Li, D., & Rahman, I.
Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Maxi (60900)

Title Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1
Journal Nature Communications. 2022.
Authors Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu & Wen Yuan
Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi
Title Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1
Journal Nature Communications. 2022.
Authors Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu, and Wen Yuan

Cell Culture Media Exosome Purification and RNA Isolation Kits

Norgen's Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

For sample input volumes ranging from 5 mL to 10 mL.

Cell Culture Media Exosome Purification and RNA Isolation Midi Kit

For sample input volumes ranging from 10 mL to 20 mL.

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

For sample input volumes ranging from 20 mL to 35 mL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 60700
Kit Specifications
Sample Type
Cell-Free Cell Culture Media
Sample Volume Range
5 mL to 10 mL
Size of RNA Purified
All sizes, including miRNA and small RNA (<200 nt) 
Elution Volume
50-100 µL
Time to Complete 10 Purifications
35 to 40 minutes
Average Yields*
Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 60800
Kit Specifications
Sample Type
Cell-Free Cell Culture Media
Sample Volume Range
10 mL to 20 mL
Size of RNA Purified
All sizes, including miRNA
and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields*
Variable depending on specimen

*Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60ºC if any salt precipitation is observed.

You have selected: Cat. 60900
Kit Specifications
Sample Type Cell-Free Cell Culture Media
Sample Volume Range 20 mL to 35 mL
Size of RNA Purified All sizes, including small RNA
(<200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications 40 to 45 minutes
Average Yields* Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component Cat. 60700 (50 preps) Cat. 60800 (25 preps) Cat. 60900 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 1.5 mL 1.5 mL 1.5 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Column 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution tubes (1.7 mL) 100 50 30
Product Insert 1 1 1

Mini (60700)

Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Midi (60800)

Title Small RNA-sequence analysis of plasma-derived extracellular vesicle miRNAs in smokers and patients with chronic obstructive pulmonary disease as circulating biomarkers
Journal Journal of Extracellular Vesicles, 8(1). 2019.
Authors Sundar, I. K., Li, D., & Rahman, I.
Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Maxi (60900)

Title Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1
Journal Nature Communications. 2022.
Authors Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu & Wen Yuan
Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi
Title Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1
Journal Nature Communications. 2022.
Authors Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu, and Wen Yuan

 


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