Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit (Cat. 60900)

Purification and enrichment of intact cell culture media exosomes for functional studies
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
Bind and elute all RNA irrespective of size or GC content, without bias
Versatile cell culture media input volume (20 mL - 35 mL)
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins

Items	Cat. #	Size
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit	60900	15 preps

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 20 mL to 35 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	20 mL to 35 mL
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40 to 45 minutes
Average Yields*	Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25ºC) for up to 2 year without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Kit Components

Component	Cat. 60900 (15 preps)
Slurry E	12.5 mL
ExoC Buffer	1.5 mL
ExoR Buffer	12 mL
Lysis Buffer A	20 mL
Lysis Additive B	2 mL
Wash Solution A	18 mL
Elution Solution A	6 mL
Mini Filter Spin Column	15
Mini Spin Columns	15
Collection Tubes	15
Elution tubes (1.7 mL)	30
Product Insert	1