|Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit||60900||15 preps|
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 20 mL to 35 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
|Sample Type||Cell-Free Cell Culture Media|
|Sample Volume Range||20 mL to 35 mL|
|Size of RNA Purified||All sizes, including small RNA
|Elution Volume||50-100 μL|
|Time to Complete 10 Purifications||40 to 45 minutes|
|Average Yields*||Variable depending on specimen|
* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25ºC) for up to 2 year without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
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