Cell Culture Media Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cell Culture Media Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media SKU 60700 Format Mini Midi Maxi Size 50 Preps Contact Distributor Overview Details Documentation Citations Overview Purification and enrichment of intact cell culture media exosomes for functional studies Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. Bind and elute all RNA irrespective of size or GC content, without bias Versatile cell culture media input volumes No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes required No precipitation reagents nor overnight incubation required Pure exosomes are purified and are free-from any other RNA-binding proteins Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen's Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Cell Culture Media Exosome Purification and RNA Isolation Mini Kit For sample input volumes ranging from 5 mL to 10 mL. Cell Culture Media Exosome Purification and RNA Isolation Midi Kit For sample input volumes ranging from 10 mL to 20 mL. Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit For sample input volumes ranging from 20 mL to 35 mL. Details Supporting Data Previous Figure 1. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification and RNA Isolation Mini Kit (Cat# 60700) was used to isolate exosomal RNA from exosomes purified from different cell culture media volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA (A) The exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90% Figure 2. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification and RNA Isolation Midi Kit (Cat# 60800) was used to isolate exosomal RNA from exosomes purified from different cell culture media volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolatedexosomal RNA. (A) The exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%. Figure 3. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit (Cat# 60900) was used to isolate exosomal RNA from different cell culture media volumes from exosomes purified using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA. (A) The exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%. Next Click for expanded view Kit Specifications Sample Type Cell-Free Cell Culture Media Sample Volume Range 5 mL to 10 mL Size of RNA Purified All sizes, including miRNA and small RNA (<200 nt) Elution Volume 50-100 µL Time to Complete 10 Purifications 35 to 40 minutes Average Yields* Variable depending on specimen * Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Component Cat. 60700 (50 preps) Cat. 60800 (25 preps) Cat. 60900 (15 preps) Slurry E 12.5 mL 12.5 mL 12.5 mL ExoC Buffer 1.5 mL 1.5 mL 1.5 mL ExoR Buffer 12 mL 12 mL 12 mL Lysis Buffer A 20 mL 20 mL 20 mL Lysis Additive B 2 mL 2 mL 2 mL Wash Solution A 18 mL 18 mL 18 mL Elution Solution A 6 mL 6 mL 6 mL Mini Filter Spin Column 50 25 15 Mini Spin Columns 50 25 15 Collection Tubes 50 25 15 Elution tubes (1.7 mL) 100 50 30 Product Insert 1 1 1 Documentation PROTOCOLS (60700) Cell Culture Media Exosome Purification and RNA Isolation Mini Kit - Protocol (50 prep) (60800) Cell Culture Media Exosome Purification and RNA Isolation Midi Kit - Protocol (25 prep) (60900) Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit - Protocol (15 prep) SAFETY DATA SHEETS (60700, 60800, 60900) Cell Culture Media Exosome Purification and RNA Isolation Kit - Safety Data Sheet POSTERS RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products Exosomal RNA Isolation Kit
Cell Culture Media Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cell Culture Media Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media SKU 60700 Format Mini Midi Maxi Size 50 Preps Contact Distributor