Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit (Cat. 60900)

Products
Exosome Isolation Kits
Intact Exosome Purification
 

Purchasing Information

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volume (20 mL - 35 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit (Cat. 60900) 15 preps

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volume (20 mL - 35 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

Click for expanded view


Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 20 mL to 35 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Sample Type Cell-Free Cell Culture Media
Sample Volume Range 20 mL to 35 mL
Size of RNA Purified All sizes, including small RNA
(<200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications 40 to 45 minutes
Average Yields* Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.


Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi
Title Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1
Journal Nature Communications. 2022.
Authors Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu, and Wen Yuan

Want a discount?

Create your account to receive 15% off on your first purchase!

Full Kits

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit (Cat. 60900) 15 preps

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volume (20 mL - 35 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

Click for expanded view


Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 20 mL to 35 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Sample Type Cell-Free Cell Culture Media
Sample Volume Range 20 mL to 35 mL
Size of RNA Purified All sizes, including small RNA
(<200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications 40 to 45 minutes
Average Yields* Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi
Title Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1
Journal Nature Communications. 2022.
Authors Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu, and Wen Yuan

 

Related Products