Cell Culture Media Exosome Purification and RNA Isolation Kits
All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media
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For research use only and NOT intended for in vitro diagnostics.
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Version Available Here.
Cell Culture Media Exosome Purification and RNA Isolation Kits
All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media
Overview
- Purification and enrichment of intact cell culture media exosomes for functional studies
- Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
- Versatile cell culture media input volumes
- No phenol extractions, Proteinase K treatment, nor carrier RNA required
- No time-consuming ultracentrifugation, filtration nor special syringes required
- No precipitation reagents nor overnight incubation required
- Pure exosomes are purified and are free from any other RNA-binding proteins
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin
- The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Norgen's Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit
For sample input volumes ranging from 5 mL to 10 mL.
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit
For sample input volumes ranging from 10 mL to 20 mL.
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
For sample input volumes ranging from 20 mL to 35 mL.
Details
Supporting Data
Figure 1. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification and RNA Isolation Mini Kit (Cat# 60700) was used to isolate exosomal RNA from exosomes purified from different cell culture media volumes using the same kit. 2 µL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA (A) The avg. Ct value for exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%
Figure 2. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification and RNA Isolation Midi Kit (Cat# 60800) was used to isolate exosomal RNA from exosomes purified from different cell culture media volumes using the same kit. 2 µL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolatedexosomal RNA. (A) The avg. Ct value for exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.
Figure 3. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit (Cat# 60900) was used to isolate exosomal RNA from different cell culture media volumes from exosomes purified using the same kit. 2 µL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA. (A) The avg. Ct value for exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.
Kit Specifications
|
|
Sample Type
|
Cell-Free Cell Culture Media
|
Sample Volume Range
|
5 mL to 10 mL
|
Size of RNA Purified
|
All sizes, including miRNA and small RNA (<200 nt)
|
Elution Volume |
50-100 µL
|
Time to Complete 10 Purifications
|
35 to 40 minutes
|
Average Yields*
|
Variable depending on specimen
|
* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Cat. 60700 (50 preps) | Cat. 60800 (25 preps) | Cat. 60900 (15 preps) |
---|---|---|---|
Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
ExoC Buffer | 1.5 mL | 1.5 mL | 1.5 mL |
ExoR Buffer | 12 mL | 12 mL | 12 mL |
Lysis Buffer A | 20 mL | 20 mL | 20 mL |
Lysis Additive B | 2 mL | 2 mL | 2 mL |
Wash Solution A | 18 mL | 18 mL | 18 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Mini Filter Spin Column | 50 | 25 | 15 |
Mini Spin Columns | 50 | 25 | 15 |
Collection Tubes | 50 | 25 | 15 |
Elution tubes (1.7 mL) | 100 | 50 | 30 |
Product Insert | 1 | 1 | 1 |
Documentation
FAQs
Mini, Midi, Maxi
Citations
Mini (60700)
Title | Cancer cells exploit an orphan RNA to drive metastatic progression |
Journal | Nature Medicine. 2018. |
Authors | Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi |
Midi (60800)
Title | RNA-Binding Protein Motifs Predict microRNA Secretion and Cellular Retention in Hypothalamic and Other Cell Types |
Journal | Biomedicnes. 2024. |
Authors | Wenyuan He, and Denise D. Belsham |
Title | Small RNA-sequence analysis of plasma-derived extracellular vesicle miRNAs in smokers and patients with chronic obstructive pulmonary disease as circulating biomarkers |
Journal | Journal of Extracellular Vesicles, 8(1). 2019. |
Authors | Sundar, I. K., Li, D., & Rahman, I. |
Title | Cancer cells exploit an orphan RNA to drive metastatic progression |
Journal | Nature Medicine. 2018. |
Authors | Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi |
Maxi (60900)
Title | Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1 |
Journal | Nature Communications. 2022. |
Authors | Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu & Wen Yuan |
Title | Cancer cells exploit an orphan RNA to drive metastatic progression |
Journal | Nature Medicine. 2018. |
Authors | Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi |
Title | Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1 |
Journal | Nature Communications. 2022. |
Authors | Chen Xu, Zicheng Zhang, Ning Liu, Li Li, Huajian Zhong, Ruizhe Wang, Qianghui Shi, Zifan Zhang, Leixin Wei, Bo Hu, Hao Zhang, Xiaolong Shen, Yue Wang, Yang Liu, and Wen Yuan |