Purchasing Information


  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA​
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volume (5 mL - 10 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins

ItemsCat. #Size
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit 60700 50 preps

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA​
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volume (5 mL - 10 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins


Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

Norgen's Cell Culture Media Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 5 mL to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Sample Type
Cell-Free Cell Culture Media
Sample Volume Range
5 mL to 10 mL
Size of RNA Purified
All sizes, including miRNA and small RNA (<200 nt) 
Elution Volume
50-100 µL
Time to Complete 10 Purifications
35 to 40 minutes
Average Yields*
Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.


Safety Data Sheets: 

Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

ItemsCat. #Size
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit 60700 50 preps

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA​
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Versatile cell culture media input volume (5 mL - 10 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents nor overnight incubation required
  • Pure exosomes are purified and are free-from any other RNA-binding proteins


Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

Norgen's Cell Culture Media Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes ranging from 5 mL to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Sample Type
Cell-Free Cell Culture Media
Sample Volume Range
5 mL to 10 mL
Size of RNA Purified
All sizes, including miRNA and small RNA (<200 nt) 
Elution Volume
50-100 µL
Time to Complete 10 Purifications
35 to 40 minutes
Average Yields*
Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Safety Data Sheets: 

Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

 

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