For the rapid preparation of genomic DNA from cultured cells as well as various tissue samples.

  • Rapid spin column format
  • High yield
  • Excellent quality
  • DNA ready for any application including PCR, qPCR, genotyping and more

ItemsCat. #Size
Cells and Tissue DNA Isolation Kit 53100 50 preps

Cells and Tissue DNA Isolation Kit

This kit is designed for the purification of genomic DNA from cultured cells, viruses and various tissue samples using rapid and convenient spin columns. Purified DNA is of an excellent yield and quality and is immediately ready for any downstream application including PCR, qPCR, genotyping and more. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues.

Kit Specifications
Maximum Input

20 mg of animal tissue
Up to 150 μL of viral suspension
3 x 106 cells

Column Binding Capacity > 50 μg
Average Yield 8 μg (from 1 x 106 HeLa Cells)
10 μg (from 10 mg kidney)
Elution Volume 50 - 200 μL
Analyte Purified Genomic DNA, mitochondrial DNA, viral DNA
Format Spin Column
Time to Complete 10 Purifications 30 min (cells) and 90 min (tissue)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.


Kit Components
Component Cat. 53100 (50 preps)
Lysis Buffer B 20 mL
Solution WN 18 mL
Wash Solution A 18 mL
Elution Buffer B 30 mL
Proteinase K 1.2 mL
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title A scientific treatment approach for acute mast cell leukemia: using a strategy based on next-generation sequencing data
Journal Blood Research. 2016.
Authors Youk J, Koh Y, Kim JW, Kim DY, Park H, Jung WJ, Ahn KS, Yun H, Park I, Sun CH, Lee S, Yoon SS