|Cells and Tissue DNA Isolation Kit||53100||50 preps|
Cells and Tissue DNA Isolation Kit
This kit is designed for the purification of genomic DNA from cultured cells, viruses and various tissue samples using rapid and convenient spin columns. Purified DNA is of an excellent yield and quality and is immediately ready for any downstream application including PCR, qPCR, genotyping and more. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues.
20 mg of animal tissue
|Column Binding Capacity||> 50 μg|
|Average Yield||8 μg (from 1 x 106 HeLa Cells)
10 μg (from 10 mg kidney)
|Elution Volume||50 - 200 μL|
|Analyte Purified||Genomic DNA, mitochondrial DNA, viral DNA|
|Time to Complete 10 Purifications||30 min (cells) and 90 min (tissue)|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.
|Title||A scientific treatment approach for acute mast cell leukemia: using a strategy based on next-generation sequencing data|
|Journal||Blood Research. 2016.|
|Authors||Youk J, Koh Y, Kim JW, Kim DY, Park H, Jung WJ, Ahn KS, Yun H, Park I, Sun CH, Lee S, Yoon SS|
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