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For the isolation of genomic DNA from various types of animal tissues or cell samples
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Maximum Input |
20 mg of animal tissue |
Column Binding Capacity | > 50 μg |
Average Yield | 8 μg (from 1 x 106 HeLa Cells) 10 μg (from 10 mg kidney) |
Elution Volume | 50 - 200 μL |
Analyte Purified | Genomic DNA, mitochondrial DNA, viral DNA |
Format | Spin Column |
Time to Complete 10 Purifications | 30 min (cells) and 90 min (tissue) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Kit Specifications
|
|
Maximum Tissue Input
|
3 mg animal tissue
Up to 150 μL of viral suspension 5 x 105 cells |
Column Loading Capacity
|
> 750 μL
|
Elution Volume
|
20 - 40 μL |
Analyte Purified | Genomic DNA, mitochondrial DNA, viral DNA |
Time to Complete Purification |
60 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Kit Specifications
|
|
Maximum Cells and Tissue Input
|
20 mg of animal tissue
3 x 106 cells |
Average Yield*
|
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells) |
Average Purity (OD260/280) |
1.8 - 1.9
|
Time to Complete 10 Purifications |
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Maximum Cells and Tissue Input
|
20 mg of animal tissue
3 x 106 cells |
Average Yield*
|
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells) |
Average Purity (OD260/280) |
1.8 - 1.9
|
Time to Complete 10 Purifications |
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
Supporting Data
Component | Cat. 53100 (50 preps) | Cat. 57300 (50 preps) | Cat. 59100 (50 preps) | Cat. 62500 (192 preps) |
---|---|---|---|---|
Lysis Buffer B | 20 mL | 20 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
Solution WN | 18 mL | 18 mL | 18 mL | 55 mL |
Wash Solution A | 18 mL | 18 mL | - | - |
Elution Buffer B | 30 mL | 8 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
Proteinase K | 1.2 mL | 1.2 mL | 1.2 mL | - |
Proteinase K in Sotrage Buffer | - | - | - | 4 mL |
Magnetic Bead Suspension | - | - | 2 x 1.1 mL | 8.5 mL |
96-Well Plate | - | - | - | 2 |
Spin Columns | 50 | - | - | - |
Micro Spin Columns | - | 50 | - | - |
Collection Tubes | 50 | 50 | - | - |
Elution Tubes (1.7 mL) | 50 | 50 | 50 | - |
96-Well Elution Plate | - | - | - | 2 |
Adhesive Tape | - | - | - | 2 |
Product Insert | 1 | 1 | 1 | 1 |
Spin Column (53100)
Title | Generation of an induced pluripotent stem cell line from a compound heterozygous patient in TK2 gene |
Journal | Stem Cell Research. 2022. |
Authors | Carmen Hernández-Ainsa, Andrés Nascimento, Cristina Jou, Rafael Artuch, Julio Montoya, Eduardo Ruiz-Pesini, Sonia Emperador |
Title | A scientific treatment approach for acute mast cell leukemia: using a strategy based on next-generation sequencing data |
Journal | Blood Research. 2016. |
Authors | Youk J, Koh Y, Kim JW, Kim DY, Park H, Jung WJ, Ahn KS, Yun H, Park I, Sun CH, Lee S, Yoon SS |
Cells and Tissue DNA Isolation Kits
Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Maximum Input |
20 mg of animal tissue |
Column Binding Capacity | > 50 μg |
Average Yield | 8 μg (from 1 x 106 HeLa Cells) 10 μg (from 10 mg kidney) |
Elution Volume | 50 - 200 μL |
Analyte Purified | Genomic DNA, mitochondrial DNA, viral DNA |
Format | Spin Column |
Time to Complete 10 Purifications | 30 min (cells) and 90 min (tissue) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Kit Specifications
|
|
Maximum Tissue Input
|
3 mg animal tissue
Up to 150 μL of viral suspension 5 x 105 cells |
Column Loading Capacity
|
> 750 μL
|
Elution Volume
|
20 - 40 μL |
Analyte Purified | Genomic DNA, mitochondrial DNA, viral DNA |
Time to Complete Purification |
60 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Kit Specifications
|
|
Maximum Cells and Tissue Input
|
20 mg of animal tissue
3 x 106 cells |
Average Yield*
|
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells) |
Average Purity (OD260/280) |
1.8 - 1.9
|
Time to Complete 10 Purifications |
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Maximum Cells and Tissue Input
|
20 mg of animal tissue
3 x 106 cells |
Average Yield*
|
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells) |
Average Purity (OD260/280) |
1.8 - 1.9
|
Time to Complete 10 Purifications |
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
Component | Cat. 53100 (50 preps) | Cat. 57300 (50 preps) | Cat. 59100 (50 preps) | Cat. 62500 (192 preps) |
---|---|---|---|---|
Lysis Buffer B | 20 mL | 20 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
Solution WN | 18 mL | 18 mL | 18 mL | 55 mL |
Wash Solution A | 18 mL | 18 mL | - | - |
Elution Buffer B | 30 mL | 8 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
Proteinase K | 1.2 mL | 1.2 mL | 1.2 mL | - |
Proteinase K in Sotrage Buffer | - | - | - | 4 mL |
Magnetic Bead Suspension | - | - | 2 x 1.1 mL | 8.5 mL |
96-Well Plate | - | - | - | 2 |
Spin Columns | 50 | - | - | - |
Micro Spin Columns | - | 50 | - | - |
Collection Tubes | 50 | 50 | - | - |
Elution Tubes (1.7 mL) | 50 | 50 | 50 | - |
96-Well Elution Plate | - | - | - | 2 |
Adhesive Tape | - | - | - | 2 |
Product Insert | 1 | 1 | 1 | 1 |
Spin Column (53100)
Title | Generation of an induced pluripotent stem cell line from a compound heterozygous patient in TK2 gene |
Journal | Stem Cell Research. 2022. |
Authors | Carmen Hernández-Ainsa, Andrés Nascimento, Cristina Jou, Rafael Artuch, Julio Montoya, Eduardo Ruiz-Pesini, Sonia Emperador |
Title | A scientific treatment approach for acute mast cell leukemia: using a strategy based on next-generation sequencing data |
Journal | Blood Research. 2016. |
Authors | Youk J, Koh Y, Kim JW, Kim DY, Park H, Jung WJ, Ahn KS, Yun H, Park I, Sun CH, Lee S, Yoon SS |