Cells and Tissue DNA Isolation Kits

Cat. 53100, 57300, 59100, 62500
  • Professional Tech Support with Senior R&D Scientist
  • Fast and Flexible Ordering Options (online, email, or phone)
  • Volume Discounts Available on Large Quantity Orders*

*Inquire by phone or email for more information.


Cells and Tissue DNA Isolation Kits

Cat. 53100, 57300, 59100, 62500

For the isolation of genomic DNA from various types of animal tissues or cell samples

  • Fast, reproducible and easy processing of samples
  • High yield and high quality genomic DNA with no RNA or protein contamination
  • DNA ready for any application including PCR, qPCR, genotyping and more

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Cells and Tissue DNA Isolation Kit
(Cat. 53100)
50 preps
Cells and Tissue DNA Isolation Micro Kit
(Cat. 57300)
50 preps
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
(Cat. 59100)
50 preps
Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System)
(Cat. 62500)
2 x 96-well plates

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Fast, reproducible and easy processing of samples
  • High yield and high quality genomic DNA with no RNA or protein contamination
  • DNA ready for any application including PCR, qPCR, genotyping and more

Cells and Tissue DNA Isolation Kits

Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Cells and Tissue DNA Isolation Kit

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Micro Kit

Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.

Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.

Kit Specifications

You have selected: Cat. 53100
Kit Specifications
Maximum Input

20 mg of animal tissue
Up to 150 μL of viral suspension
3 x 106 cells

Column Binding Capacity > 50 μg
Average Yield 8 μg (from 1 x 106 HeLa Cells)
10 μg (from 10 mg kidney)
Elution Volume 50 - 200 μL
Analyte Purified Genomic DNA, mitochondrial DNA, viral DNA
Format Spin Column
Time to Complete 10 Purifications 30 min (cells) and 90 min (tissue)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

You have selected: Cat. 57300
Kit Specifications
Maximum Tissue Input
3 mg animal tissue
Up to 150 μL of viral suspension
5 x 105 cells
Column Loading Capacity
> 750 μL
Elution Volume
20 - 40 μL
Analyte Purified Genomic DNA, mitochondrial DNA, viral DNA
Time to Complete Purification
60 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

You have selected: Cat. 59100
Kit Specifications
Maximum Cells and Tissue Input
20 mg of animal tissue
3 x 106 cells
Average Yield*
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells)
Average Purity (OD260/280)
1.8 - 1.9
Time to Complete 10 Purifications
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 62500
Kit Specifications
Maximum Cells and Tissue Input
20 mg of animal tissue
3 x 106 cells
Average Yield*
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells)
Average Purity (OD260/280)
1.8 - 1.9
Time to Complete 10 Purifications
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 53100 (50 preps) Cat. 57300 (50 preps) Cat. 59100 (50 preps) Cat. 62500 (192 preps)
Lysis Buffer B 20 mL 20 mL 20 mL 1 x 40 mL
1 x 20 mL
Solution WN 18 mL 18 mL 18 mL 55 mL
Wash Solution A 18 mL 18 mL - -
Elution Buffer B 30 mL 8 mL 15 mL 1 x 30 mL
1 x 15 mL
Proteinase K 1.2 mL 1.2 mL 1.2 mL -
Proteinase K in Sotrage Buffer - - - 4 mL
Magnetic Bead Suspension - - 2 x 1.1 mL 8.5 mL
96-Well Plate - - - 2
Spin Columns 50 - - -
Micro Spin Columns - 50 - -
Collection Tubes 50 50 - -
Elution Tubes (1.7 mL) 50 50 50 -
96-Well Elution Plate - - - 2
Adhesive Tape - - - 2
Product Insert 1 1 1 1

Regular (53100)

Title Generation of an induced pluripotent stem cell line from a compound heterozygous patient in TK2 gene
Journal Stem Cell Research. 2022.
Authors Carmen Hernández-Ainsa, Andrés Nascimento, Cristina Jou, Rafael Artuch, Julio Montoya, Eduardo Ruiz-Pesini, Sonia Emperador
Title A scientific treatment approach for acute mast cell leukemia: using a strategy based on next-generation sequencing data
Journal Blood Research. 2016.
Authors Youk J, Koh Y, Kim JW, Kim DY, Park H, Jung WJ, Ahn KS, Yun H, Park I, Sun CH, Lee S, Yoon SS

Cells and Tissue DNA Isolation Kits

Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Cells and Tissue DNA Isolation Kit

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Micro Kit

Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.

Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 53100
Kit Specifications
Maximum Input

20 mg of animal tissue
Up to 150 μL of viral suspension
3 x 106 cells

Column Binding Capacity > 50 μg
Average Yield 8 μg (from 1 x 106 HeLa Cells)
10 μg (from 10 mg kidney)
Elution Volume 50 - 200 μL
Analyte Purified Genomic DNA, mitochondrial DNA, viral DNA
Format Spin Column
Time to Complete 10 Purifications 30 min (cells) and 90 min (tissue)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

You have selected: Cat. 57300
Kit Specifications
Maximum Tissue Input
3 mg animal tissue
Up to 150 μL of viral suspension
5 x 105 cells
Column Loading Capacity
> 750 μL
Elution Volume
20 - 40 μL
Analyte Purified Genomic DNA, mitochondrial DNA, viral DNA
Time to Complete Purification
60 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

You have selected: Cat. 59100
Kit Specifications
Maximum Cells and Tissue Input
20 mg of animal tissue
3 x 106 cells
Average Yield*
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells)
Average Purity (OD260/280)
1.8 - 1.9
Time to Complete 10 Purifications
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 62500
Kit Specifications
Maximum Cells and Tissue Input
20 mg of animal tissue
3 x 106 cells
Average Yield*
8-10 µg (20 mg of animal tissue)
8-12 µg (3 x 106 cells)
Average Purity (OD260/280)
1.8 - 1.9
Time to Complete 10 Purifications
40 minutes hands-on time (Cat. 59100)
50 minutes hands on time (Cat. 62500)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component Cat. 53100 (50 preps) Cat. 57300 (50 preps) Cat. 59100 (50 preps) Cat. 62500 (192 preps)
Lysis Buffer B 20 mL 20 mL 20 mL 1 x 40 mL
1 x 20 mL
Solution WN 18 mL 18 mL 18 mL 55 mL
Wash Solution A 18 mL 18 mL - -
Elution Buffer B 30 mL 8 mL 15 mL 1 x 30 mL
1 x 15 mL
Proteinase K 1.2 mL 1.2 mL 1.2 mL -
Proteinase K in Sotrage Buffer - - - 4 mL
Magnetic Bead Suspension - - 2 x 1.1 mL 8.5 mL
96-Well Plate - - - 2
Spin Columns 50 - - -
Micro Spin Columns - 50 - -
Collection Tubes 50 50 - -
Elution Tubes (1.7 mL) 50 50 50 -
96-Well Elution Plate - - - 2
Adhesive Tape - - - 2
Product Insert 1 1 1 1

Regular (53100)

Title Generation of an induced pluripotent stem cell line from a compound heterozygous patient in TK2 gene
Journal Stem Cell Research. 2022.
Authors Carmen Hernández-Ainsa, Andrés Nascimento, Cristina Jou, Rafael Artuch, Julio Montoya, Eduardo Ruiz-Pesini, Sonia Emperador
Title A scientific treatment approach for acute mast cell leukemia: using a strategy based on next-generation sequencing data
Journal Blood Research. 2016.
Authors Youk J, Koh Y, Kim JW, Kim DY, Park H, Jung WJ, Ahn KS, Yun H, Park I, Sun CH, Lee S, Yoon SS

 


Related Products