Figure 2. Isolation of High Quality Genomic DNA from 0.1 up to 3 million mammalian cells. Genomic DNA was isolated from 0.1 x 10⁶ to up to 3 million HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit. Triplicate samples were used from each cell input and yielded genomic DNA of the highest quality and integrity with linear increase in yield with increasing cell input. Lane M is the Norgen UltraRanger 1kb DNA Ladder. For all purified DNA, 15 µL of each 200 µL elution were resolved on a 1X TAE, 1% agarose DNA gel.

Figure 3. Yield of Genomic DNA from 0.1 x 10⁶ up to 3 x 10⁶ mammalian cells. Genomic DNA was isolated from 0.1 up to 3 million HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit. Triplicate samples were used from each cell input and 25 µL of the 200 µL elution were diluted in 475 µL of nuclease-free water, and DNA concentrations were measured using the UltraSpec 2100 Pro (Fisher Scientific). Linear increase in DNA yield was obtained when increasing the cell input from 0.1 x 10⁶ - 3 x 10⁶ HeLa cells.

Figure 4. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. Genomic DNA was isolated from 0.1 x 10⁶ to 3 x 10⁶ of HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit. Five µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene from all the isolated DNA from the different cell inputs with a decreasing Ct value with increasing the cell input. This indicates that the kit provides high quality DNA in each case and is free of PCR inhibitors making it well suited to sensitive downstream applications.

Figure 5. Resolution of DNA isolated from Animal Tissue and Mammalian Cells. DNA was isolated from 20 mg animal tissue (beef) and 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System), and Norgen's Cells and Tissue DNA Kit (column format, Cat. 53100) as a control. The DNA isolated with the Magnetic Bead System (B) was then compared with the column method (C). For evaluation, 10 µL from each 200 µL of elution were run on 1X TAE 1.2% agarose gel. As it can be seen, Norgen's Magnetic Bead System (B) was able to isolate high yields of DNA from both the tissue and cells. M = Norgen's HighRanger DNA Ladder.

Figure 6. Comparison of A260/280 and A260/230 ratios. DNA was isolated from 20 mg animal tissue (beef) and 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System), and Norgen's Cells and Tissue DNA Kit (column format, Cat. 53100). The DNA isolated with the Magnetic Bead System was then compared with the column method for A260/280 and A260/230 rations. DNA isolated using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System) showed a comparable DNA quality to the column method (Cat. 53100).

Figure 7. High Quality DNA was confirmed by Real-time PCR. DNA was isolated from 20 mg animal tissue (beef) and 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation Kit (Magnetic Bead System). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL (circle), 4 µL (triangle) and 8 µL cross) of animal tissue DNA or HeLa DNA (total PCR reaction volume was 20 µL) to detect 5s rDNA or GAPDH from animal tissue and HeLa cell respectably. No PCR inhibition was observed from the all different PCR template amounts, indicating the excellent quality of animal tissue and HeLa DNA for downstream applications.

Figure 8. DNA was Isolated from 1 x 10⁶ HeLa Cells Using Norgen's Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from each 100 µL of elution were run on 1X TAE 1.2% agarose gel. As it can be seen, Norgen's Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) was able to isolate consistent and high yields of DNA from HeLa cells. M = Norgen's HighRanger DNA Ladder (Cat. 11900).

Figure 9. High Quality DNA was Confirmed by Real-Time PCR. DNA was isolated from 1 x 10⁶ HeLa cells using Norgen's Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL of HeLa DNA (total PCR reaction volume was 20 µL) to detect GAPDH from HeLa cell. No PCR inhibition was observed indicating the excellent quality of HeLa DNA for downstream applications.