For Whole Blood Collection and Preservation of Cell-Free Circulating DNA and RNA

  • Fixative-free preservative, no cross-linking of DNA
  • Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C
  • Preserve cf-RNA for 30 days at ambient temperature
  • Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
  • No plasma volume loss after shipping/transportation
  • Prevent hemolysis allowing better separation of plasma
  • Prevent apoptosis of blood cells and fragmentation of genomic DNA
  • Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
  • Vacuumed to draw 8.4 mL of blood in 10 mL tubes

ItemsCat. #Size
cf-DNA/cf-RNA Preservative Tubes 63950 50 Tubes
cf-DNA/cf-RNA Preservative Tubes 63960 100 Tubes

cf-DNA/cf-RNA Preservative Tubes

Norgen's cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen's cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.

This product is for research use only and not for use in diagnostic procedures.

Performance

Norgen's cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen's cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.

Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.

Workflow

Collection and Mixing Instructions

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NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950

Kit Specifications
Tube Size
16 x 100 mm
Tube Type PET Tube
Blood Draw Volume
8.4 mL in 10 mL Tubes
Preservative Volume
1.6 mL
Recovered Plasma Volume Approximately 6-7 mL
(not affected by shipping)
Anticoagulant
Proprietary
Preservative
Non formaldehyde - Proprietary preservation agent

Length of cf-DNA Preservation

Up to 30 days at room temperature (15-25°C)
Up to 8 days at 37°C

Length of cf-RNA Preservation

Up to 30 days at room temperature (15-25°C)

Length of CTCs Preservation

Up to 14 days at room temperature (15-25°C)

Storage Conditions and Product Stability

  1. When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes are stable through the expiration date. See tube label for expiry date.
  2. Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes.
  3. Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
  4. Cell-free DNA in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
  5. Cell-free DNA in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes is stable for up to 8 days when stored at 37ºC.
  6. Cell-free RNA in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
  7. Circulating tumor cells (CTCs) in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes are stable for up to 14 days at room temperature (15-25ºC).


Kit Components
Component Cat. 63950 (50 tubes) Cat. 63960 (100 tubes)
cf-DNA/cf-RNA Preservative Tubes 50 100
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