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For Whole Blood Collection and Preservation of Cell-Free Circulating DNA and RNA
For research use only and NOT intended for in vitro diagnostics.
CE-IVD diagnostic version available here
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Kit Specifications
Kit Specifications
|
|
Tube Size
|
16 x 100 mm
|
Tube Type | PET Tube |
Blood Draw Volume
|
8.4 mL in 10 mL Tubes
|
Preservative Volume |
1.6 mL
|
Recovered Plasma Volume | Approximately 6-7 mL (not affected by shipping) |
Anticoagulant |
Proprietary
|
Preservative |
Non formaldehyde - Proprietary preservation agent
|
Length of cf-DNA Preservation |
Up to 30 days at room temperature (15-25°C)
Up to 8 days at 37°C |
Length of cf-RNA Preservation |
Up to 30 days at room temperature (15-25°C)
|
Length of CTCs Preservation |
Up to 14 days at room temperature (15-25°C)
|
Storage Conditions and Product Stability
Supporting Data
Title | From Sampling to Sequencing: A Liquid Biopsy Pre-Analytic Workflow to Maximize Multi-Layer Genomic Information from a Single Tube |
Journal | Cancers. 2021. |
Authors | Maass, Kendra K., Paulina S. Schad, Agnes ME Finster, Pitithat Puranachot, Fabian Rosing, Tatjana Wedig, Nathalie Schwarz, Natalie Stumpf, Stefan M. Pfister, and Kristian W. Pajtler |
Title | Response to BRAF/MEK Inhibition in A598_T599insV BRAF Mutated Melanoma |
Journal | Case Reports in Oncology. 2019. |
Authors | Sara Bjursten, Christoffer Vannas, Stefan Filges, Florian Puls, Ankur Pandita, Henrik Fagman, Anders Ståhlberg, and Max Levin |
Title | Evaluation of Storage Tubes for Combined Analysis of Circulating Nucleic Acids in Liquid Biopsies |
Journal | International Journal of Molecular Sciences. 2019. |
Authors | Aoife Ward Gahlawat, Judith Lenhardt, Tania Witte, Denise Keitel, Anna Kaufhold, Kendra K Maass, Kristian W Pajtler, Christof Sohn, Sarah Schott |
Title | 554P KRAS-dependent and independent mechanisms of progressive disease (PD) in colorectal cancer (CRC) patients (pts) with liver metastases (LM) while monitoring on circulating cell free DNA (cfDNA) |
Journal | Annals of Oncology. October 23, 2018. |
Authors | A Kirov Z Mihaylova, V Petrova, T Todorov, D Petkova, A Garev, A Todorova-Georgieva |
Documents | Poster |
cf-DNA/cf-RNA Preservative Tubes
Norgen's cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen's cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.
This product is for research use only and not for use in diagnostic procedures.
Performance
Norgen's cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen's cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.
Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.
Workflow
Collection & Mixing Tutorial - cf-DNA/cf-RNA Preservative Tubes
NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950
Infographic
Supporting Data
Kit Specifications
Kit Specifications
|
|
Tube Size
|
16 x 100 mm
|
Tube Type | PET Tube |
Blood Draw Volume
|
8.4 mL in 10 mL Tubes
|
Preservative Volume |
1.6 mL
|
Recovered Plasma Volume | Approximately 6-7 mL (not affected by shipping) |
Anticoagulant |
Proprietary
|
Preservative |
Non formaldehyde - Proprietary preservation agent
|
Length of cf-DNA Preservation |
Up to 30 days at room temperature (15-25°C)
Up to 8 days at 37°C |
Length of cf-RNA Preservation |
Up to 30 days at room temperature (15-25°C)
|
Length of CTCs Preservation |
Up to 14 days at room temperature (15-25°C)
|
Storage Conditions and Product Stability
Title | From Sampling to Sequencing: A Liquid Biopsy Pre-Analytic Workflow to Maximize Multi-Layer Genomic Information from a Single Tube |
Journal | Cancers. 2021. |
Authors | Maass, Kendra K., Paulina S. Schad, Agnes ME Finster, Pitithat Puranachot, Fabian Rosing, Tatjana Wedig, Nathalie Schwarz, Natalie Stumpf, Stefan M. Pfister, and Kristian W. Pajtler |
Title | Response to BRAF/MEK Inhibition in A598_T599insV BRAF Mutated Melanoma |
Journal | Case Reports in Oncology. 2019. |
Authors | Sara Bjursten, Christoffer Vannas, Stefan Filges, Florian Puls, Ankur Pandita, Henrik Fagman, Anders Ståhlberg, and Max Levin |
Title | Evaluation of Storage Tubes for Combined Analysis of Circulating Nucleic Acids in Liquid Biopsies |
Journal | International Journal of Molecular Sciences. 2019. |
Authors | Aoife Ward Gahlawat, Judith Lenhardt, Tania Witte, Denise Keitel, Anna Kaufhold, Kendra K Maass, Kristian W Pajtler, Christof Sohn, Sarah Schott |
Title | 554P KRAS-dependent and independent mechanisms of progressive disease (PD) in colorectal cancer (CRC) patients (pts) with liver metastases (LM) while monitoring on circulating cell free DNA (cfDNA) |
Journal | Annals of Oncology. October 23, 2018. |
Authors | A Kirov Z Mihaylova, V Petrova, T Todorov, D Petkova, A Garev, A Todorova-Georgieva |
Documents | Poster |