CleanAll DNA/RNA Clean-Up and Concentration Micro Kit For the rapid and efficient purification, cleanup and concentration of RNA or DNA For research use only and NOT intended for in vitro diagnostics. Powered by Bioz CleanAll DNA/RNA Clean-Up and Concentration Micro Kit For the rapid and efficient purification, cleanup and concentration of RNA or DNA Price USD$255.00 SKU 23800 Format Micro Size 50 Preps Quantity Overview Details Documentation Citations Overview Can be used for the clean up of both RNA and DNA from enzymatic reactions, labeling etc. Purifies all sizes of RNA, from large mRNA down to microRNA (miRNA) Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA Removes endotoxins for transfection of injection ready RNA or DNA Rapid and efficient spin-column format (20 minutes) Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen's CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions. The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less. Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified. Details Supporting Data Previous Figure 1. High Recoveries and Efficient Endotoxin Removal. High recoveries of DNA can be seen when using the CleanAll kit, and these high recoveries are not compromised even during endotoxin removal. In Panel A, 1 µg of a PCR product was cleaned using the CleanAll DNA/RNA Clean-Up and Concentration Micro Kit as well as Norgen's PCR Purification Kit. More than 0.9 µg of the DNA was recovered from both kits with a comparable percent recovery of over 90%. In Panel B, 10 µg of plasmid DNA preparation was cleaned from endotoxin by using the CleanAll DNA/RNA Kit. Endotoxin levels of the plasmid DNA sample were reduced from 0.75 EU/µg plasmid DNA to less 0.05 EU/µg plasmid DNA. Approximately 95% of the endotoxin units in the DNA sample were removed using the kit, therefore the kit allows for high recoveries coupled with exceptional cleanup. Figure 2: Clean-Up of RNA with High Recovery. Norgen's CleanAll DNA/RNA Clean-Up and Concentration Micro Kit can be used to clean up various enzymatic reactions including DNase treatment. Lane 1 is the RNA input, while lanes 2-5 contain the RNA that has been cleaned using Norgen's CleanAll Kit. It can be seen that in all cases the recovery is high, and the purified RNA is intact and of a high quality. Next Click for expanded view Kit Specifications Column Binding Capacity 50 μg RNA 10 μg DNA Maximum Column Loading Volume 600 μL Minimum Elution Volume 20 μL Time to Complete 10 Purifications 20 minutes Size of RNA Purified All sizes, including small RNA (<200) Size of DNA Purified ≥ 100 bp* Average Recovery ≥90% for RNA ≥90% for DNA 100 bp to 10 kbp ≥75% for DNA ≥ 10 kbp * Applicable to smaller fragments or oligonucleotides with alternative protocol Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. ComponentCat. 23800 (50 preps)Binding Buffer H60 mLWash Solution K27 mLElution Buffer L15 mLMicro Spin Columns50Collection Tubes50Elution Tubes (1.7 mL)50Product Insert1 Documentation PROTOCOLS PI23800-11 CleanAll Kit Insert.pdf SAFETY DATA SHEETS (23800) CleanAll DNA/RNA Clean-up and Concentration Micro Kit - Safety Data Sheet POSTERS Effect of DNA Contaminants On Transfection Efficiency and Mutation Rate In Cho Cells - ASCB 2007 FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Title Microbial succession in a marine sediment: Inferring interspecific microbial interactions with marine cable bacteria Journal Environmental Microbiology. 2016. Authors Pinky Liau, Carol Kim, Matthew A. Saxton, Sairah Y. Malkin Title Microbial N transformations and N2O emission after simulated grassland cultivation: Effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) Journal Applied and Environmental Microbiology. 2016. Authors Duan YF, Kong XW, Schramm A, Labouriau R, Eriksen J, Petersen SO. Title 3,4-Dimethylpyrazole phosphate (DMPP) reduces activity of ammonia oxidizers without adverse effects on non-target soil microorganisms and functions Journal Applied Soil Ecology. 2016. Authors Kong, X., Duan, Y., Schramm, A., Eriksen, J., & Petersen, S. O. Title Bioengineering Novel Chimeric microRNA-34a for Prodrug Cancer Therapy: High-Yield Expression and Purification, and Structural and Functional Characterization Journal Journal of Pharmacology and Experimental Therapeutics. 2015. Authors Wang WP, Ho PY, Chen QX, Addepalli B, Limbach PA, Li MM, Wu WJ, Jilek JL, Qiu JX, Zhang HJ, Li T, Wun T, White RD, Lam KS, Yu AM Title A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types Journal Frontiers in Microbiology. 2015. Authors Mark Alexander Lever, Andrea Torti, Philip Eickenbusch, Alexander Bryce Michaud, Tina Šantl-Temkiv and Bo Barker Jørgensen Title Establishment of a minigenome system for Oropouche orthobunyavirus reveals the S genome segment to be significantly longer than previously reported Journal Journal of General Virology. 2014. Authors Gustavo Olszanski Acrani1, Natasha L Tilston-Lunel2, Martin Spiegel3, Manfred Weidemann4, Meik Dilcher5, Daisy Elaine Andrade da Silva6, Marcio R.T. Nunes7 and Richard M. Elliott Title Site-and horizon-specific patterns of microbial community structure and enzyme activities in permafrost-affected soils of Greenland. Journal Frontiers in Microbiology. 2014. Authors Antje Gittel, Jiri Barta, Iva Lacmanova, Joerg Schnecker, Birgit Wild, Petr Capek, Christina Kaiser, Vigdis Torsvik, Andreas Richter, Christa Schleper and Tim Urich. Title Distinct microbial communities associated with buried soils in the Siberian tundra. Journal RNA Biology. 2013. Authors Gittel A, Bárta J, Kohoutová I, Mikutta R, Owens S, Gilbert J, Schnecker J, Wild B, Hannisdal B, Maerz 8, Lashchinskiy N, Capek P, Santrucková H, Gentsch N, Shibistova O, Guggenberger G, Richter A, Torsvik VL, Schleper C, Urich T. Title Genetic characterization of Erve virus, a European Nairovirus distantly related to Crimean-Congo hemorrhagic fever virus. Journal Virus Genes. 2012. Authors Dilcher M, Koch A, Hasib L, Dobler G, Hufert FT, Weidmann M. Title Placental Expression of CD100, CD72 and CD45 Is Dysregulated in Human Miscarriage. Journal PLOS One. 2012. Authors Lorenzi T, Turi A, Lorenzi M, Paolinelli F, Mancioli F Sala L, Morroni M, Ciarmela P, Mantovani A, Tranquilli A, Castellucci M, Marzioni D. Title Effect of DNA Contaminants on Calcium Phosphate-Based DNA Delivery and Gene Expression. Journal Journal of Biotech Research. 2012. Authors El-Mogy M and Haj-Ahmad Y. Title Epigenetic Differences in Normal Colon Mucosa of Cancer Patients Suggest Altered Dietary Metabolic Pathways. Journal Cancer Preventation Research. Philidelphia (PA). 2012. Authors Silviera ML, Smith BP, Powell J, Sapienza C. Related Products Total RNA Purification Kit Cytoplasmic & Nuclear RNA Purification Kit Leukocyte RNA Purification Kit microRNA Purification Kit
CleanAll DNA/RNA Clean-Up and Concentration Micro Kit For the rapid and efficient purification, cleanup and concentration of RNA or DNA For research use only and NOT intended for in vitro diagnostics. Powered by Bioz CleanAll DNA/RNA Clean-Up and Concentration Micro Kit For the rapid and efficient purification, cleanup and concentration of RNA or DNA Price USD$255.00 SKU 23800 Format Micro Size 50 Preps Quantity