For the rapid and efficient purification, cleanup and concentration of RNA or DNA
|CleanAll DNA/RNA Clean-Up and Concentration Micro Kit||23800||50 preps|
CleanAll DNA/RNA Clean-Up and Concentration Micro Kit
Norgen's CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions.
The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less.
Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified.
|Column Binding Capacity||
50 μg RNA
10 μg DNA
|Maximum Column Loading Volume||
|Minimum Elution Volume||
|Time to Complete 10 Purifications||
|Size of RNA Purified||
All sizes, including small RNA (<200)
|Size of DNA Purified||
≥ 100 bp*
≥90% for RNA
≥90% for DNA 100 bp to 10 kbp
≥75% for DNA ≥ 10 kbp
* Applicable to smaller fragments or oligonucleotides with alternative protocol
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||Microbial N transformations and N2O emission after simulated grassland cultivation: Effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP)|
|Journal||Applied and Environmental Microbiology. 2016.|
|Authors||Duan YF, Kong XW, Schramm A, Labouriau R, Eriksen J, Petersen SO.|
|Title||3,4-Dimethylpyrazole phosphate (DMPP) reduces activity of ammonia oxidizers without adverse effects on non-target soil microorganisms and functions|
|Journal||Applied Soil Ecology. 2016.|
|Authors||Kong, X., Duan, Y., Schramm, A., Eriksen, J., & Petersen, S. O.|
|Title||Bioengineering Novel Chimeric microRNA-34a for Prodrug Cancer Therapy: High-Yield Expression and Purification, and Structural and Functional Characterization|
|Journal||Journal of Pharmacology and Experimental Therapeutics. 2015.|
|Authors||Wang WP, Ho PY, Chen QX, Addepalli B, Limbach PA, Li MM, Wu WJ, Jilek JL, Qiu JX, Zhang HJ, Li T, Wun T, White RD, Lam KS, Yu AM|
|Title||A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types|
|Journal||Frontiers in Microbiology. 2015.|
|Authors||Mark Alexander Lever, Andrea Torti, Philip Eickenbusch, Alexander Bryce Michaud, Tina Šantl-Temkiv and Bo Barker Jørgensen|
|Title||Establishment of a minigenome system for Oropouche orthobunyavirus reveals the S genome segment to be significantly longer than previously reported|
|Journal||Journal of General Virology. 2014.|
|Authors||Gustavo Olszanski Acrani1, Natasha L Tilston-Lunel2, Martin Spiegel3, Manfred Weidemann4, Meik Dilcher5, Daisy Elaine Andrade da Silva6, Marcio R.T. Nunes7 and Richard M. Elliott|
|Title||Site-and horizon-specific patterns of microbial community structure and enzyme activities in permafrost-affected soils of Greenland.|
|Journal||Frontiers in Microbiology. 2014.|
|Authors||Antje Gittel, Jiri Barta, Iva Lacmanova, Joerg Schnecker, Birgit Wild, Petr Capek, Christina Kaiser, Vigdis Torsvik, Andreas Richter, Christa Schleper and Tim Urich.|
|Title||Distinct microbial communities associated with buried soils in the Siberian tundra.|
|Journal||RNA Biology. 2013.|
|Authors||Gittel A, Bárta J, Kohoutová I, Mikutta R, Owens S, Gilbert J, Schnecker J, Wild B, Hannisdal B, Maerz 8, Lashchinskiy N, Capek P, Santrucková H, Gentsch N, Shibistova O, Guggenberger G, Richter A, Torsvik VL, Schleper C, Urich T.|
|Title||Genetic characterization of Erve virus, a European Nairovirus distantly related to Crimean-Congo hemorrhagic fever virus.|
|Journal||Virus Genes. 2012.|
|Authors||Dilcher M, Koch A, Hasib L, Dobler G, Hufert FT, Weidmann M.|
|Title||Placental Expression of CD100, CD72 and CD45 Is Dysregulated in Human Miscarriage.|
|Journal||PLOS One. 2012.|
|Authors||Lorenzi T, Turi A, Lorenzi M, Paolinelli F, Mancioli F Sala L, Morroni M, Ciarmela P, Mantovani A, Tranquilli A, Castellucci M, Marzioni D.|
|Title||Effect of DNA Contaminants on Calcium Phosphate-Based DNA Delivery and Gene Expression.|
|Journal||Journal of Biotech Research. 2012.|
|Authors||El-Mogy M and Haj-Ahmad Y.|
|Title||Epigenetic Differences in Normal Colon Mucosa of Cancer Patients Suggest Altered Dietary Metabolic Pathways.|
|Journal||Cancer Preventation Research. Philidelphia (PA). 2012.|
|Authors||Silviera ML, Smith BP, Powell J, Sapienza C.|
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