Includes a 2686 bp (pUC19) reference band for easy clone identification

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
  • Higher intensity reference bands at 500 bp and 1000 bp
  • 2686 bp (pUC19) reference band for easy clone identification

ItemsCat. #Size
Clone Sizer 100 bp DNA Ladder 11600 100 loads

CloneSizer 100 bp DNA Ladder

The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

CloneSizer 100 bp DNA Ladder (Cat# 11600) - 100 loads

Ladder Properties:
  • Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
  • Higher intensity reference bands at 500 bp and 1000 bp
  • 2686 bp (pUC19) reference band for easy clone identification
Fragment
Size (bp)
Mass (ng)
1
2686

72

2
2000
53
3
1500
41
4
1200
42
5
1000
56
6
900
30
7
800
29
8
700
25
9
600
25
10
500
52
11
400
19
12 300

20

13 200

19

14 100

17

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Title PCR Based Screening of Fusarium sp. in some Organic Products
Journal Bulletin UASVM Animal Science and Biotechnologies. 2015.
Authors Oana M. BOLDURA, Petrica BOZAC, Sorina POPESCU
Title Bacterial community shift in the coastal Gulf of Mexico salt-marsh sediment microcosm in vitro following exposure to the Mississippi Canyon Block 252 oil (MC252).
Journal 3 Biotech. 2014.
Authors Hyunmin Koo, Nazia Mojib, Jonathan P. Huang, Rona J. Donahoe, Asim K. Bej.
Title UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2.
Journal Extremophiles: Life Under Extreme Conditions. 2013.
Authors Mojib N, Farhoomand A, Andersen DT, Bej AK.
Title Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR.
Journal Antonie Van Leeuwenhoek 97(3):275-87.. 2010.
Authors Panicker G, Mojib N, Aislabie J, Bej AK.
Title Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays.
Journal Appl Environ Microbiol.. 2005.
Authors Panicker G, Call DR, Krug MJ, Bej AK.
Title PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico.
Journal Appl Environ Microbiol. 2003 Apr;69(4):2194-200.
Authors Myers ML, Panicker G, Bej AK.