CloneSizer 100 bp DNA Ladder (Cat. 11600)
CloneSizer 100 bp DNA Ladder
Cat. 11600
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CloneSizer 100 bp DNA Ladder
Cat. 11600
Includes a 2686 bp (pUC19) reference band for easy clone identification
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Fourteen discrete fragments ranging from 100Â bp to 2000Â bp in 100Â bp increments
- Higher intensity reference bands at 500Â bp and 1000Â bp
- 2686Â bp (pUC19) reference band for easy clone identification
For research use only and NOT intended for in vitro diagnostics.
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Clone Sizer 100 bp DNA Ladder
(Cat. 11600) 100 loads
(Cat. 11600) 100 loads
Product Size
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Kit Specifications
You have selected: Cat. 11600
CloneSizer 100 bp DNA Ladder (Cat# 11600) - 100 loads
Ladder Properties:
- Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
- Higher intensity reference bands at 500 bp and 1000 bp
- 2686 bp (pUC19) reference band for easy clone identification
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
2686
|
72 |
|
2
|
2000
|
53
|
|
3
|
1500
|
41
|
|
4
|
1200
|
42
|
|
5
|
1000
|
56
|
|
6
|
900
|
30
|
|
7
|
800
|
29
|
|
8
|
700
|
25
|
|
9
|
600
|
25
|
|
10
|
500
|
52
|
|
11
|
400
|
19
|
| 12 | 300 |
20 |
| 13 | 200 |
19 |
| 14 | 100 |
17 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Supporting Data
Click for expanded view
Protocols:
Application Notes:
Safety Data Sheets:
| Title | PCR Based Screening of Fusarium sp. in some Organic Products |
| Journal | Bulletin UASVM Animal Science and Biotechnologies. 2015. |
| Authors | Oana M. BOLDURA, Petrica BOZAC, Sorina POPESCU |
| Title | Bacterial community shift in the coastal Gulf of Mexico salt-marsh sediment microcosm in vitro following exposure to the Mississippi Canyon Block 252 oil (MC252). |
| Journal | 3 Biotech. 2014. |
| Authors | Hyunmin Koo, Nazia Mojib, Jonathan P. Huang, Rona J. Donahoe, Asim K. Bej. |
| Title | UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2. |
| Journal | Extremophiles: Life Under Extreme Conditions. 2013. |
| Authors | Mojib N, Farhoomand A, Andersen DT, Bej AK. |
| Title | Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR. |
| Journal | Antonie Van Leeuwenhoek 97(3):275-87.. 2010. |
| Authors | Panicker G, Mojib N, Aislabie J, Bej AK. |
| Title | Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes. |
| Journal | Appl Environ Microbiol.. 2006. |
| Authors | Ward LN, Bej AK. |
| Title | Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays. |
| Journal | Appl Environ Microbiol.. 2005. |
| Authors | Panicker G, Call DR, Krug MJ, Bej AK. |
| Title | PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico. |
| Journal | Appl Environ Microbiol. 2003 Apr;69(4):2194-200. |
| Authors | Myers ML, Panicker G, Bej AK. |
CloneSizer 100 bp DNA Ladder
The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100Â bp to 2000Â bp in 100Â bp increments, double intensity reference bands at 500 and 1000Â bp and an additional 2686Â bp (pUC19) reference band for easy clone identification.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Supporting Data
Click for expanded view
Kit Specifications
You have selected: Cat. 11600
CloneSizer 100 bp DNA Ladder (Cat# 11600) - 100 loads
Ladder Properties:
- Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
- Higher intensity reference bands at 500 bp and 1000 bp
- 2686 bp (pUC19) reference band for easy clone identification
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
2686
|
72 |
|
2
|
2000
|
53
|
|
3
|
1500
|
41
|
|
4
|
1200
|
42
|
|
5
|
1000
|
56
|
|
6
|
900
|
30
|
|
7
|
800
|
29
|
|
8
|
700
|
25
|
|
9
|
600
|
25
|
|
10
|
500
|
52
|
|
11
|
400
|
19
|
| 12 | 300 |
20 |
| 13 | 200 |
19 |
| 14 | 100 |
17 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Protocols:
Application Notes:
Safety Data Sheets:
| Title | PCR Based Screening of Fusarium sp. in some Organic Products |
| Journal | Bulletin UASVM Animal Science and Biotechnologies. 2015. |
| Authors | Oana M. BOLDURA, Petrica BOZAC, Sorina POPESCU |
| Title | Bacterial community shift in the coastal Gulf of Mexico salt-marsh sediment microcosm in vitro following exposure to the Mississippi Canyon Block 252 oil (MC252). |
| Journal | 3 Biotech. 2014. |
| Authors | Hyunmin Koo, Nazia Mojib, Jonathan P. Huang, Rona J. Donahoe, Asim K. Bej. |
| Title | UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2. |
| Journal | Extremophiles: Life Under Extreme Conditions. 2013. |
| Authors | Mojib N, Farhoomand A, Andersen DT, Bej AK. |
| Title | Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR. |
| Journal | Antonie Van Leeuwenhoek 97(3):275-87.. 2010. |
| Authors | Panicker G, Mojib N, Aislabie J, Bej AK. |
| Title | Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes. |
| Journal | Appl Environ Microbiol.. 2006. |
| Authors | Ward LN, Bej AK. |
| Title | Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays. |
| Journal | Appl Environ Microbiol.. 2005. |
| Authors | Panicker G, Call DR, Krug MJ, Bej AK. |
| Title | PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico. |
| Journal | Appl Environ Microbiol. 2003 Apr;69(4):2194-200. |
| Authors | Myers ML, Panicker G, Bej AK. |