Cryptococcus neoformans TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cryptococcus neoformans TaqMan PCR Detection Kits Price USD$699.00 SKU TM42750 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Cryptococcus neoformans Available in TaqMan format for analysis Cryptococcus neoformans is an encapsulated yeast. Infection with C. neoformans is known as cryptococcosis and is the cause of the most common life-threatening meningitis in patients with weakened immune systems, particularly in advanced HIV/AIDS. C. neoformans is commonly found in soil throughout the world. Human infection of C. neoformans occurs via inhalation of aerosolized spores. From the lungs, C. neoformans is spread hematogenously to the Central Nervous System (CNS), resulting in meningoencephalitis. Although the availability of antiretroviral therapy in the developed world has reduced the incidents of cryptococcosis, it is still a major problem in developing countries and is one of the leading causes of death in patients with HIV/AIDS. In fact, one of the major challenges in treating cryptococcosis is that many patients with cryptococcal CNS disease are asymptomatic in terms of cryptococcal pneumonia, making it difficult for early detection. TaqMan PCR Kit Click to expand options Cryptococcus neoformans TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Cryptococcus neoformans TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM42750 (100 preps) Cat. TM42710 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Cryptococcus neoformans Primer & Probe Mix 280 μL 280 μL Cryptococcus neoformans Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM42750) Cryptococcus neoformans TaqMan PCR Kit - Protocol (100 rxns) SAFETY DATA SHEETS (TM42750) Cryptococcus neoformans TaqMan PCR Kit - Safety Data Sheet Citations Title Commercial Molecular Tests for Fungal Diagnosis from a Practical Point of View Journal Methods in Molecular Biology. 2016. Authors Lackner M, Lass-Flörl C. Related Products Cryptococcus neoformans TaqMan Probe/Primer and Control Set Blood DNA Isolation Mini Kit
Cryptococcus neoformans TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cryptococcus neoformans TaqMan PCR Detection Kits Price USD$699.00 SKU TM42750 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity