Cryptosporidium TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Cryptosporidium TaqMan RT-PCR Detection Kit Dx Price USD$349.00 SKU DxTM39100 Format RT-PCR Size 24 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Cryptosporidium CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available Available in Taqman Probe format for analysis Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection. TaqMan PCR Kit Click to expand options Cryptosporidium TaqMan RT-PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions The Cryptosporidium TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. DxTM39100 (24 rxns) MDx TaqMan 2X RT-PCR Master Mix Dx 550 µL Cryptosporidium Primer & Probe Mix Dx 2 x 70 µL Cryptosporidium Positive Control Dx - 200,000 μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM39100) Cryptosporidium TaqMan RT-PCR Kit Dx - Protocol (24 rxns) Citations Powered by Bioz See more details on Bioz Related Products CSVd TaqMan Probe/Primer and Control Set Water RNA/DNA Purification Kit - 0.22 µm
Cryptosporidium TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Cryptosporidium TaqMan RT-PCR Detection Kit Dx Price USD$349.00 SKU DxTM39100 Format RT-PCR Size 24 Reactions Quantity