Overview

The Chrysanthemum Stunt Viroid (CSVd) is the causal agent of a disease known as chrysanthemum stunt. The viroid consists of small pieces of RNA (354 bp) that are known to be highly contagious. The main symptoms of CSVd infection are severe stunting of the plants such that they are half to two-thirds of the normal size, as well as smaller flowers and premature flowering. Symptoms of Chrysanthemum Stunt are difficult to detect in stock, however can be so severe at flowering that finished crops of flowers are unmarketable. There is no chemical that can control CSVd, therefore much effort has been made to generate chrysanthemum plants free from CSVd. Norgen’s Chrysanthemum Stunt Viroid Detection Kits are a rapid and sensitive CSVd detection tool which can be applicable for the early diagnosis of CSVd.

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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Documentation

Related Products

Chrysanthemum Stunt Viroid (CSVd) TaqMan Probe/Primer and Control Set

Plant/Fungi Total RNA Purification Kit