• Detection kits for CSVd
  • Available in 2 formats for analysis:
 

ItemsCat. #Size
CSVd TaqMan RT-PCR Kit TM39900 24 rxns
CSVd TaqMan Probe/Primer and Control Set TM39910 100 rxns
CSVd End-Point RT-PCR Kit EP39900 24 rxns
CSVd Primer and Control Set EP39910 100 rxns

CSVd Detection Kits

The Chrysanthemum Stunt Viroid (CSVd) is the causal agent of a disease known as chrysanthemum stunt. The viroid consists of small pieces of RNA (354 bp) that are known to be highly contagious. The main symptoms of CSVd infection are severe stunting of the plants such that they are half to two-thirds of the normal size, as well as smaller flowers and premature flowering. Symptoms of Chrysanthemum Stunt are difficult to detect in stock, however can be so severe at flowering that finished crops of flowers are unmarketable. There is no chemical that can control CSVd, therefore much effort has been made to generate chrysanthemum plants free from CSVd. Norgen’s Chrysanthemum Stunt Viroid Detection Kits are a rapid and sensitive CSVd detection tool which can be applicable for the early diagnosis of CSVd.

There are 2 kits available for the detection of CSVd:

CSVd TaqMan PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
CSVd End-Point PCR Kit
  • Master Mix and primers for the specific amplification of a 342 bp region of the CSVd genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM39900
(24 rxns)
End-Point
Cat. EP39900
(24 rxns)
MDx 2X RT-PCR Master Mix - 350 µL
MDx SYBR 2X RT-PCR Master Mix - -
MDx SYBR 2X RT-PCR Control Master Mix - -
MDx TaqMan 2X RT-PCR Master Mix 350 µL -
CSVd Primer Mix 70 µL 70 µL
CSVd Positive Control 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Loading Dye - 100 µL
DNA Ladder - 100 µL
Product Insert 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM39910
(100 rxns)
Primer
and Control Set
Cat. EP39910
(100 rxns)
CSVd Primer Mix 3 x 70 µL 3 x 70 µL
CSVd Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1