For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues

  • Excellent separation and purification of cytoplasmic and nuclear RNA
  • Convenient and fast spin column format
  • High quality and yield of RNA
  • Isolate full diversity of RNA (including microRNA) without phenol
  • Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
  • Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR

ItemsCat. #Size
Cytoplasmic & Nuclear RNA Purification Kit 21000 50 preps
Cytoplasmic & Nuclear RNA Purification Kit 37400 100 preps

Cytoplasmic and Nuclear RNA Purification Kit

This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.

Background

In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.

Kit Specifications
Binding Capacity Per Column
Up to 50 µg RNA
Maximum Loading Volume Per Spin Column
650 µL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
45 minutes
RNA Yield
HeLa (1 x 106) - Cytoplasmic RNA
HeLa (1 x 106) - Nuclear RNA
15 µg
≤ 3.5 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 21000 (50 preps) Cat. 37400 (100 preps)
Lysis Buffer J 20 mL 2 x 20 mL
Buffer SK 40 mL 2 x 40 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Buffer E 6 mL 2 x 6 mL
Mini Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1

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Title Extracellular Vesicles Including Exosomes Regulate Innate Immune Responses to Hepatitis B Virus Infection
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Title Developmental regulation of human cortex transcription and its clinical relevance at single base resolution.
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Title Long Noncoding RNA-EBIC Promotes Tumor Cell Invasion by Binding to EZH2 and Repressing E-Cadherin in Cervical Cancer.
Journal PLoS ONE. 2014.
Authors Ning-xia Sun, Chen Ye, Qian Zhao, Qing Zhang, Chen Xu, Shao-bing Wang, Zhi-jun Jin, Shu-han Sun, Fang Wang, Wen Li mail
Title Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene.
Journal Molecular Biotechnology. 2014.
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Title Strain-level genomic variation in natural populations of Lebetimonas from an erupting deep-sea volcano.
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Title Dengue virus subgenomic RNA induces apoptosis through the Bcl-2-mediated PI3k/Akt signaling pathway.
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Title Identification of a long non-coding RNA-associated RNP complex regulating metastasis at the translational step
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Title MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability.
Journal Nucleic Acids Research. 2013.
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Title Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus.
Journal Proceedings of the National Academy of Sciences of the United States of America. 2013.
Authors Lee H, Komano J, Saitoh Y, Yamaoka S, Kozaki T, Misawa T, Takahama M, Satoh T, Takeuchi O, Yamamoto N, Matsuura Y, Saitoh T, Akira S.
Title DNA-Damage-Induced Nuclear Export of Precursor MicroRNAs Is Regulated by the ATM-AKT Pathway.
Journal Cell. 2013.
Authors Wan G,Zhang X, Langley R, Liu Y, Hu X, Han C, Peng G, Ellis L, Jones S, Lu X
Title Btf and TRAP150 have distinct roles in regulating subcellular mRNA distribution.
Journal Nucleus. 2013.
Authors Varia S, Potabathula D, Deng Z, Bubulya A, Bubulya PA.
Title Nuclear Phosphoinositide-Specific Phospholipase C β1 Controls Cytoplasmic CCL2 mRNA Levels in HIV-1 gp120-Stimulated Primary Human Macrophages.
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Authors Spadaro F, Cecchetti S, Purificato C, Sabbatucci M, Podo F, Ramoni C, Gessani S, Fantuzzi L.
Title Circular RNAs are abundant, conserved, and associated with ALU repeats.
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Authors Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF, Sharpless NE.
Title Long non-coding antisense RNA controls Uchl1 translation through an embedded SINEB2 repeat.
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Title Characterization of Novel Paternal ncRNAs at the Plagl1 Locus, Including Hymai, Predicted to Interact with Regulators of Active Chromatin.
Journal PLoS One. 2012.
Authors Iglesias-Platas I, Martin-Trujillo A, Cirillo D, Court F, Guillaumet-Adkins A, Camprubi C, Bourc'his D, Hata K, Feil R, Tartaglia G, Arnaud P, Monk D.
Title MxA inhibits hepatitis B virus replication by interaction with core protein HBcAg.
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Authors Li N, Zhang L, Chen L, Feng W, Xu Y, Chen F, Liu X, Chen Z, Liu W.
Title Reduced DICER1 elicits an interferon response in endometrial cancer cells.
Journal Molecular Cancer Research. 2012.
Authors Chiappinelli KB, Haynes BC, Brent MR, Goodfellow PJ.
Title Monoallelic Expression Determines Oncogenic Progression and Outcome in Benign and Malignant Brain Tumors.
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Authors Walker EJ, Zhang C, Castelo-Branco P, Hawkins C, Wilson W, Zhukova N, Alon N, Novokmet A, Baskin B, Ray P, Knobbe C, Dirks P, Taylor MD, Croul S, Malkin D, Tabori U.
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Title Small Noncoding RNA Modulates Japanese Encephalitis Virus Replication and Translation in trans.
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Title Strand antagonism in RNAi: an explanation of differences in potency between intracellularly expressed siRNA and shRNA.
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Title Nuclear and cytoplasmic localization of neural stem cell microRNAs.
Journal RNA 2011.
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Title Gene processing control loops suggested by sequencing, splicing, and RNA folding.
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Title The Nuclear Transcription Factor RARa Associates with Neuronal RNA Granules and Suppresses Translation.
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Title Mutation in Nuclear Pore Component NUP155 Leads to Atrial Fibrillation and Early Sudden Cardiac Death
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