Cytoplasmic and Nuclear RNA Purification Kit (Cat. 21000, 37400)

For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues

  • Excellent separation and purification of cytoplasmic and nuclear RNA
  • Convenient and fast spin column format
  • High quality and yield of RNA
  • Isolate full diversity of RNA (including microRNA) without phenol
  • Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
  • Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR

ItemsCat. #Size
Cytoplasmic & Nuclear RNA Purification Kit 21000 50 preps
Cytoplasmic & Nuclear RNA Purification Kit 37400 100 preps

Cytoplasmic and Nuclear RNA Purification Kit

This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol.   Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations.   Ten samples can be processed in approximately 45 minutes.  This kit is also available in a 100 prep size.    

Background

In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA. 

Kit Specifications
Binding Capacity Per Column
Up to 50 µg RNA
Maximum Loading Volume Per Spin Column
650 µL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
45 minutes
RNA Yield
HeLa (1 x 106) - Cytoplasmic RNA
HeLa (1 x 106) - Nuclear RNA
15 µg
≤ 3.5 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.
 

Title C-MYC-induced upregulation of lncRNA SNHG12 regulates cell proliferation, apoptosis and migration in triple-negative breast cancer
Journal Am J Transl Res. 2017
Authors Ouchen Wang, Fan Yang, Yehuan Liu, Lin Lv, Ruimin Ma, Chuanzhi Chen, Jiao Wang, Qiufan Tan,Yue Cheng, Erjie Xia, Yizuo Chen, Xiaohua Zhang
Title Long noncoding RNA MHENCR promotes melanoma progression via regulating miR-425/489-mediated PI3K-Akt pathway
Journal American Journal of Translational Research. 2017.
Authors Chen X, Dong H, Liu S, Yu L, Yan D, Yao X, Sun W, Han D, Gao G.
Title Neuronal hemoglobin affects dopaminergic cells’ response to stress
Journal Cell Death and Disease. 2017.
Authors Codrich M, Bertuzzi M, Russo R, Francescatto M, Espinoza S, Zentilin L, Giacca M, Cesselli D, Beltrami AP, Ascenzi P, Zucchelli S,, Persichetti F, Leanza G, Gustincich S
Title Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1
Journal Nature Communications. 2016.
Authors Kishikawa T, Otsuka M,, Yoshikawa T, Ohno M, Ijichi H, Koike K.
Title Extracellular Vesicles Including Exosomes Regulate Innate Immune Responses to Hepatitis B Virus Infection
Journal Frontiers in Immunology. 2016.
Authors Kouwaki T, Fukushima Y, Daito T, Sanada T, Yamamoto N, Mifsud EJ, Leong CR, Tsukiyama-Kohara K, Kohara M, Matsumoto M, Seya T, Oshiumi H
Title The microRNA cluster miR-183/96/182 contributes to long-term memory in a protein phosphatase 1-dependent manner
Journal Nature Communications. 2016.
Authors Woldemichael BT, Jawaid A, Kremer EA, Gaur N, Krol J, Marchais A, Mansuy IM
Title Long noncoding RNA GPC3-AS1 promotes hepatocellular carcinoma progression via epigenetically activating GPC3
Journal The FEBS Journal. 2016.
Authors Zhu XT, Yuan JH, Zhu TT, Li YY, Cheng XY
Title Global transcript structure resolution of high gene density genomes through multi-platform data integration
Journal Nucleic Acids Research. 2016.
Authors O'Grady T, Wang X, Höner Zu Bentrup K, Baddoo M, Concha M, Flemington EK
Title Long noncoding RNA GIHCG promotes hepatocellular carcinoma progression through epigenetically regulating miR-200b/a/429
Journal Journal of Molecular Medicine. 2016.
Authors Sui CJ, Zhou YM, Shen WF, Dai BH, Lu JJ, Zhang MF, Yang JM
Title miR-34 activity is modulated through 5′-end phosphorylation in response to DNA damage
Journal Nature Communications. 2016.
Authors Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack FJ, Weidhaas JB
Title Long noncoding RNA FGFR3-AS1 promotes osteosarcoma growth through regulating its natural antisense transcript FGFR3
Journal Molecular Biology Reports. 2016.
Authors Sun, J., Wang, X., Fu, C., Wang, X., Zou, J., Hua, H., & Bi, Z.
Title Genetic predisposition to neuroblastoma mediated by a LMO1 super-enhancer polymorphism
Journal Nature. 2015.
Authors Derek A. Oldridge, Andrew C. Wood, Nina Weichert-Leahey, Ian Crimmins, Robyn Sussman, Cynthia Winter, Lee D. McDaniel, Maura Diamond, Lori S. Hart, Shizhen Zhu, Adam D. Durbin, Brian J. Abraham, Lars Anders, Lifeng Tian, Shile Zhang, Jun S. Wei, Javed Khan, Kelli Bramlett, Nazneen Rahman, Mario Capasso, Achille Iolascon, Daniela S. Gerhard, Jaime M. Guidry Auvil, Richard A. Young, Hakon Hakonarson et al.
Title Long Non-coding RNA Growth Arrest-specific Transcript 5 (GAS5) Inhibits Liver Fibrogenesis through a Mechanism of Competing Endogenous RNA
Journal The Journal of Biological Chemistry. 2015.
Authors Fujun Yu, Jianjian Zheng, Yuqing Mao, Peihong Dong, Zhongqiu Lu, Guojun Li, Chuanyong Guo, Zhanju Liu and Xiaoming Fan
Title The Role of Long Intergenic Noncoding RNA p21 in Vascular Endothelial Cells
Journal DNA and Cell Biology. 2015.
Authors He C, Ding JW, Li S, Wu H, Jiang YR, Yang W, Teng L, Yang J, Yang J
Title Regulation of p53 during senescence in normal human keratinocytes
Journal Aging Cell. 2015.
Authors Kim RH, Kang MK, Kim T, Yang P, Bae S, Williams DW, Phung S, Shin KH, Hong C, Park NH
Title New non-coding lytic transcripts derived from the Epstein Barr virus latency origin of replication oriP are hyper-edited, bind the paraspeckle protein, NONO/p54nrb, and support lytic viral transcription.
Journal Journal of Virology. 2015.
Authors Cao S, Moss W, O'Grady T, Concha M, Strong MJ, Wang X, Yu Y, Baddoo M, Zhang K, Fewell C, Lin Z, Dong Y, Flemington EK
Title A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature
Journal Nature Structural & Molecular Biology. 2015.
Authors Inma Gonzalez, Roberto Munita, Eneritz Agirre, Travis A Dittmer, Katia Gysling, Tom Misteli & Reini F Luco
Title Microtubule disruption synergizes with oncolytic virotherapy by inhibiting interferon translation and potentiating bystander killing.
Journal Nature Communications. 2015.
Authors Rozanne Arulanandam, Cory Batenchuk, Oliver Varette, Chadi Zakaria, Vanessa Garcia, Nicole E. Forbes, Colin Davis, Ramya Krishnan, Raunak Karmacharya, Julie Cox, Anisha Sinha, Andrew Babawy, KatherineWaite, EricaWeinstein, Theresa Falls, Andrew Chen, Jeff Hamill, Naomi De Silva, David P. Conrad, Harold Atkins, Kenneth Garson, Carolina Ilkow, Mads Kærn, Barbara Vanderhyden, Nahum Sonenberg, Tommy Alain, Fabrice Le Boeuf, John C. Bell1, & Jean-Simon Diallo
Title SP1-induced upregulation of the long noncoding RNA TINCR regulates cell proliferation and apoptosis by affecting KLF2 mRNA stability in gastric cancer.
Journal Oncogene. 2015.
Authors Xu TP, Liu XX, Xia R, Yin L, Kong R, Chen WM, Huang MD, Shu YQ.
Title Developmental regulation of human cortex transcription and its clinical relevance at single base resolution.
Journal Nature Neuroscience. 2014.
Authors Andrew E Jaffe, Jooheon Shin, Leonardo Collado-Torres, Jeffrey T Leek, Ran Tao, Chao Li, Yuan Gao, Yankai Jia, Brady J Maher, Thomas M Hyde, Joel E Kleinman & Daniel R Weinberger.
Title High-Throughput RNA sequencing based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project.
Journal Journal of Virology. 2014.
Authors Cao S, Strong MJ, Wang X, Moss WN, Concha M, Lin Z, O'Grady T, Baddoo M, Fewell C, Renne R, Flemington EK.
Title Long Noncoding RNA-EBIC Promotes Tumor Cell Invasion by Binding to EZH2 and Repressing E-Cadherin in Cervical Cancer.
Journal PLoS ONE. 2014.
Authors Ning-xia Sun, Chen Ye, Qian Zhao, Qing Zhang, Chen Xu, Shao-bing Wang, Zhi-jun Jin, Shu-han Sun, Fang Wang, Wen Li mail
Title Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene.
Journal Molecular Biotechnology. 2014.
Authors Sakaguchi M, Watanabe M, Kinoshita R, Kaku H, Ueki H, Futami J, Murata H, Inoue Y, Li SA, Huang P, Putranto EW, Ruma IM, Nasu Y, Kumon H, Huh NH.
Title Strain-level genomic variation in natural populations of Lebetimonas from an erupting deep-sea volcano.
Journal Journal of Virology. 2013.
Authors O'Grady T, Cao S, Strong MJ, Concha M, Wang X, Bondurant SS, Adams M, Baddoo M, Srivastav SK, Lin Z, Fewell C, Yin Q, Flemington EK.
Title Efficient cellular fractionation improves RNA sequencing analysis of mature and nascent transcripts from human tissues.
Journal BMC Biotechnology. 2013.
Authors Zaghlool A, Ameur A, Nyberg L, Halvardson J, Grabherr M, Cavelier L, Feuk L.
Title Dengue virus subgenomic RNA induces apoptosis through the Bcl-2-mediated PI3k/Akt signaling pathway.
Journal Virology. 2013.
Authors Liua Y, Liua H, Zoua J, Zhanga B and Yuana Z
Title Identification of a long non-coding RNA-associated RNP complex regulating metastasis at the translational step
Journal The EMBO Journal. 2013.
Authors Gumireddy K, Li A, Yan J, Setoyama T, Johannes GJ, Orom UA, Tchou J, Liu Q, Zhang L, Speicher DW, Calin GA, Huang Q.
Title MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability.
Journal Nucleic Acids Research. 2013.
Authors Li J, Chen Y, Qin X, Wen J, Ding H, Xia W, Li S, Su X, Wang W, Li H, Zhao Q, Fang T, Qu L, Shao N.
Title Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus.
Journal Proceedings of the National Academy of Sciences of the United States of America. 2013.
Authors Lee H, Komano J, Saitoh Y, Yamaoka S, Kozaki T, Misawa T, Takahama M, Satoh T, Takeuchi O, Yamamoto N, Matsuura Y, Saitoh T, Akira S.
Title DNA-Damage-Induced Nuclear Export of Precursor MicroRNAs Is Regulated by the ATM-AKT Pathway.
Journal Cell. 2013.
Authors Wan G,Zhang X, Langley R, Liu Y, Hu X, Han C, Peng G, Ellis L, Jones S, Lu X
Title Btf and TRAP150 have distinct roles in regulating subcellular mRNA distribution.
Journal Nucleus. 2013.
Authors Varia S, Potabathula D, Deng Z, Bubulya A, Bubulya PA.
Title Nuclear Phosphoinositide-Specific Phospholipase C β1 Controls Cytoplasmic CCL2 mRNA Levels in HIV-1 gp120-Stimulated Primary Human Macrophages.
Journal PLoS One. 2013.
Authors Spadaro F, Cecchetti S, Purificato C, Sabbatucci M, Podo F, Ramoni C, Gessani S, Fantuzzi L.
Title Circular RNAs are abundant, conserved, and associated with ALU repeats.
Journal RNA: A Publication of the RNA Society. 2013.
Authors Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF, Sharpless NE.
Title Long non-coding antisense RNA controls Uchl1 translation through an embedded SINEB2 repeat.
Journal Nature. 2012.
Authors Carrieri C, Cimatti L, Biagioli M, Beugnet A, Zucchelli S, Fedele S, Pesce E, Ferrer I, Collavin L, Santoro C, Forrest AR, Carninci P, Biffo S, Stupka E, Gustincich S.
Title Characterization of Novel Paternal ncRNAs at the Plagl1 Locus, Including Hymai, Predicted to Interact with Regulators of Active Chromatin.
Journal PLoS One. 2012.
Authors Iglesias-Platas I, Martin-Trujillo A, Cirillo D, Court F, Guillaumet-Adkins A, Camprubi C, Bourc'his D, Hata K, Feil R, Tartaglia G, Arnaud P, Monk D.
Title MxA inhibits hepatitis B virus replication by interaction with core protein HBcAg.
Journal Hepatology. 2012.
Authors Li N, Zhang L, Chen L, Feng W, Xu Y, Chen F, Liu X, Chen Z, Liu W.
Title Reduced DICER1 elicits an interferon response in endometrial cancer cells.
Journal Molecular Cancer Research. 2012.
Authors Chiappinelli KB, Haynes BC, Brent MR, Goodfellow PJ.
Title Monoallelic Expression Determines Oncogenic Progression and Outcome in Benign and Malignant Brain Tumors.
Journal Cancer Research. 2011.
Authors Walker EJ, Zhang C, Castelo-Branco P, Hawkins C, Wilson W, Zhukova N, Alon N, Novokmet A, Baskin B, Ray P, Knobbe C, Dirks P, Taylor MD, Croul S, Malkin D, Tabori U.
Title Identification of new viral genes and transcript isoforms during Epstein-Barr virus reactivation using RNA-seq.
Journal Journal of Virology. 2011.
Authors Concha M, Wang X, Cao S, Baddoo M, Fewell C, Lin Z, Hulme W, Hedges D, McBride J, Flemington EK.
Title Small Noncoding RNA Modulates Japanese Encephalitis Virus Replication and Translation in trans.
Journal Virology Journal. 2011.
Authors Fan YH, Nadar M, Chen CC, Weng CC, Lin YT, Chang RY.
Title Strand antagonism in RNAi: an explanation of differences in potency between intracellularly expressed siRNA and shRNA.
Journal Nucleic Acids Resarch. 2011.
Authors Jin X, Sun T, Zhao C, Zheng Y, Zhang Y, Cai W, He Q, Taira K, Zhang L, Zhou D.
Title Nuclear and cytoplasmic localization of neural stem cell microRNAs.
Journal RNA 2011.
Authors Jeffries CD, Fried HM, Perkins DO.
Title Gene processing control loops suggested by sequencing, splicing, and RNA folding.
Journal BMC Bioformatics 2010.
Authors Jeffries CD, Perkins DO, Guan X.
Title A genetic defect in exportin-5 traps precursor microRNAs in the nucleus of cancer cells
Journal Cancer Cell 2010.
Authors >Melo SA, Moutinho C, Ropero S, Calin GA, Rossi S, Spizzo R, Fernandez AF, Davalos V, Villanueva A, Montoya G, Yamamoto H, Schwartz S Jr, Esteller M.
Title Molecular Interplay of the Noncoding RNA ANRIL and Methylated Histone H3 Lysine 27 by Polycomb CBX7 in Transcriptional Silencing of INK4a.
Journal Mol Cell. 2010.
Authors Yap KL, Li S, Muñoz-Cabello AM, Raguz S, Zeng L, Mujtaba S, Gil J, Walsh MJ, Zhou MM.
Title The obesity-associated SNPs in intron 1 of the FTO gene affect primary transcript levels.
Journal Eur J Hum Genet. 2010.
Authors Berulava T, Horsthemke B.
Title The Nuclear Transcription Factor RARa Associates with Neuronal RNA Granules and Suppresses Translation.
Journal Journal of Biological Chemistry. 283: 20841 - 20847
Authors Chen N, Onisko B, Napoli J.
Title Mutation in Nuclear Pore Component NUP155 Leads to Atrial Fibrillation and Early Sudden Cardiac Death
Journal Cell.   135: 1017-1027
Authors Zhang X, Chen S, Yoo S, Chakrabarti S, Zhang T, Ke T, Oberti C, Yong SL, Fang F, Li L, de la Fuente R, Wang L, Chen Q,
Title Down-regulation of tissue inhibitor of metalloproteinase-3 (TIMP-3) expression is necessary for adipocyte differentiation.
Journal Journal of Biological Chemistry. 2010.
Authors Bernot D, Barruet E, Poggi M, Bonardo B, Alessi MC, Peiretti F.