Cytoplasmic and Nuclear RNA Purification Kit (Cat. 21000, 37400)
Cytoplasmic and Nuclear RNA Purification Kit
Cat. 21000, 37400
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Cytoplasmic and Nuclear RNA Purification Kit
Cat. 21000, 37400
For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues
- Excellent separation and purification of cytoplasmic and nuclear RNA
- Convenient and fast spin column format
- High quality and yield of RNA
- Isolate full diversity of RNA (including microRNA) without phenol
- Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
- Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
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Cytoplasmic & Nuclear RNA Purification Kit
(Cat. 21000) 50 preps
(Cat. 21000) 50 preps
Cytoplasmic & Nuclear RNA Purification Kit
(Cat. 37400) 100 preps
(Cat. 37400) 100 preps
Kit Size
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Price is dependent on a variety of factors including location and account type. Please sign in to your account to view more accurate pricing.
- Excellent separation and purification of cytoplasmic and nuclear RNA
- Convenient and fast spin column format
- High quality and yield of RNA
- Isolate full diversity of RNA (including microRNA) without phenol
- Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
- Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Cytoplasmic and Nuclear RNA Purification Kit
This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.
Background
In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.
RNA Purification Tutorial - Cytoplasmic and Nuclear RNA
Kit Specifications
You have selected: Cat. 21000
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Kit Specifications
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Maximum Column Binding Capacity
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Up to 50 µg RNA
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Maximum Column Loading Volume
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650 µL
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Size of RNA Purified
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All sizes, including small RNA (< 200 nt)
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| Time to Complete 10 Purifications |
45 minutes
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RNA Yield
HeLa (1 x 106) - Cytoplasmic RNA HeLa (1 x 106) - Nuclear RNA |
15 µg ≤ 3.5 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
You have selected: Cat. 37400
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
Up to 50 µg RNA
|
|
Maximum Column Loading Volume
|
650 µL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt)
|
| Time to Complete 10 Purifications |
45 minutes
|
|
RNA Yield
HeLa (1 x 106) - Cytoplasmic RNA HeLa (1 x 106) - Nuclear RNA |
15 µg ≤ 3.5 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
Figure 1. Effective Separation of HeLa Cell Cytoplasmic & Nuclear RNA. Norgen's Cytoplasmic & Nuclear RNA Purification Kit was used to effectively separate cytoplasmic and nuclear RNA from 0.75 million HeLa cells in triplicate. Panel A: High quality cytoplasmic and nuclear RNA was purified using Norgen's kit. Note the integrity and quality of both cytoplasmic and nuclear RNA. Ten microliters of each 50 µL elution were run on a 1.5% formaldehyde-agarose gel. Lane M is Norgen's 1 kb RNA Ladder, lanes 1-3 contain nuclear RNA and lanes 4-6 contain cytoplasmic RNA. Panel B: Ten microliters of the above cytoplasmic and nuclear RNA isolated from HeLa cells using Norgen's kit was run on a 0.9% agarose DNA gel. Genomic DNA is clearly visible in the nuclear RNA fractions (lanes 1-3), however, no genomic DNA can be detected in the cytoplasmic RNA fractions (lanes 4-6). Note that an optional on-column DNase treatment protocol is provided to remove the genomic DNA in the nuclear fraction.
Figure 2. Superior Separation of HeLa Cell Cytoplasmic & Nuclear RNA. Norgen's Cytoplasmic & Nuclear RNA Purification Kit provides better separation of cytoplasmic and nuclear RNA from 0.8 million HeLa cells when compared to a leading competitors product. Panel A: Cytoplasmic and nuclear RNA purified from HeLa cells using Norgen's kit and a competitor's kit. Ten microliters of each 50 µL elution (Norgen or competitor's kit) of the cytoplasmic (C) or nuclear (N) RNA were run on a 1.5% formaldehyde-agarose gel. Higher yields of RNA with good integrity were isolated using Norgen's kit. Panel B: Ten microliters of the cytoplasmic and nuclear RNA isolated from HeLa cells using Norgen's kit and the competitor's kit was run on a 0.9% agarose gel. Genomic DNA only co-migrates with the nuclear fraction in RNA isolated using Norgens Cytoplasmic & Nuclear RNA Purification Kit, not the cytoplasmic fraction. Note that an optional on-column DNase treatment protocol is provided to remove the genomic DNA in the nuclear fraction. In contrast, significant genomic DNA contamination was observed in the cytoplasmic fraction of the RNA isolated using the competitor's kit.
Figure 3. Genomic DNA-free Cytoplasmic RNA. RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Norgen's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Norgen's PCR Sizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as that from the RNA transcript. The lack of product in lanes 1 and 3 indicates that no genomic DNA contamination is present in the isolated cytoplasmic RNA. All PCR products were resolved on a 1X TAE, 2% agarose gel.
Figure 4. Specific Separation and Subsequent Amplification of Cytoplasmic and Nuclear Transcripts. Norgen's Cytoplasmic & Nuclear RNA Purification Kit effectively separates cytoplasmic and nuclear RNA. RT-PCR was performed using human U2 snRNA (Panel A) or S14 (Panel B) primers on 0.5 µg of either cytoplasmic or nuclear RNA fractions isolated from 1 million HeLa cells using Norgen's Cytoplasmic & Nuclear RNA Purification Kit. In Panel A, an intense PCR product was observed only in the nuclear fraction when the nuclear-specific U2 snRNA primers were used. In Panel B, the majority of the PCR product for the house-keeping transcript of S14 was observed in the cytoplasmic fraction. The two results combined showed effective separation of the cytoplasmic and nuclear RNA using Norgen's Cytoplasmic & Nuclear RNA Purification Kit. All PCR products were resolved on a 1X TAE, 1.5% agarose DNA gel.
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| Component | Cat. 21000 (50 preps) | Cat. 37400 (100 preps) |
|---|---|---|
| Lysis Buffer J | 20 mL | 2 x 20 mL |
| Buffer SK | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer E | 6 mL | 2 x 6 mL |
| Mini Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
Protocols:
Application Notes:
Posters:
Safety Data Sheets:
| Title | CircESRP1 enhances metastasis and epithelial–mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis |
| Journal | Journal of Translational Medicine. 2022. |
| Authors | Rui Shi, Wei Zhang, Jun Zhang, Zhicheng Yu, Lanfen An, Rong Zhao, Xing Zhou, Ziwei Wang, Sitian Wei & Hongbo Wang |
| Title | Identification of a novel circular RNA circZNF652/miR-486-5p/SERPINE1 signaling cascade that regulates cancer aggressiveness in glioblastoma (GBM) |
| Journal | Bioengineered. 2022. |
| Authors | Liang Liu, Shan Xiao, Yan Wang, Zifeng Zhu, Yiyao Cao, Sen Yang, Rongkang Mai &Yong Zheng |
| Title | A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis |
| Journal | Cell Death & Disease. 2022. |
| Authors | Yuan Tan, Chonghua Jiang, Qunying Jia, Jing Wang, Ge Huang & Faqing Tang |
| Title | Long non‑coding RNA LINC00238 suppresses the malignant phenotype of liver cancer by sponging miR‑522 |
| Journal | Molecular Medicine Reports. 2022. |
| Authors | Hong-Gang Qian, Qiong Wu, Jian-Hui Wu, Xiu-Yun Tian, Wei Xu, Chun-Yi Hao |
| Title | Coenzyme Q regulates UCP1 expression and thermogenesis through the integrated stress responses |
| Journal | bioRxiv. 2022. |
| Authors | Ching-Fang Chang, Amanda L. Gunawan, Peter-James H. Zushin, Greg A. Timblin, Ambre M. Bertholet, Biao Wang, Kaoru Sajio, Yuriy Kirichok, Andreas Stahl |
| Title | circKLHL24 Blocks Breast Cancer Development by Regulating the miR-1204/ALX4 Network |
| Journal | Cancer Biotherapy & Radiopharmaceuticals. 2022. |
| Authors | Guangming Yi, Dan Wang, Jianjun Han, Li Jia, Xiaojun Liu, and Jun He |
| Title | RBM47/SNHG5/FOXO3 axis activates autophagy and inhibits cell proliferation in papillary thyroid carcinoma |
| Journal | Cell Death & Disease. 2022. |
| Authors | Yuan Qin, Wei Sun, Zhihong Wang, Wenwu Dong, Liang He, Ting Zhang, Chengzhou Lv & Hao Zhang |
| Title | CircUCK2 regulates HECTD1-mediated endothelial-mesenchymal transition inhibition by interacting with FUS and protects the blood–brain barrier in ischemic stroke |
| Journal | The kaohsiung Journal of Medical Sciences. 2022. |
| Authors | Jun-Hong Zhuang, Huan-Xiong Chen, Ning Gao, Rong-Dao Sun, Cheng-Ye Xiao, De-Hua Zeng, Zheng-Tao Yu, Jun Peng, Ying Xia |
| Title | Long non-coding RNA muskelin 1 antisense RNA as a potential therapeutic target in hepatocellular carcinoma treatment |
| Journal | Bioengineered . 2022. |
| Authors | Xijun Chen, Qing Ye, Zhigao Chen, Qian Lin, Wen Chen, Chengrong Xie & Xiaomin Wang |
| Title | Long non-coding RNA ADAMTS9-AS1 attenuates ferroptosis by Targeting microRNA-587/solute carrier family 7 member 11 axis in epithelial ovarian cancer |
| Journal | Bioengineered . 2022. |
| Authors | Li Cai, Xiaoqing Hu, Lu Ye, Pingjuan Bai, Youkun Jie & Kuanyong Shu |
| Title | A new circular RNA–encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)–induced mitochondrial dysfunction |
| Journal | JBC. 2022. |
| Authors | Xiaomin Zhao, Jianxiong Guo, Xinyue Wang, Jiadi Lin, Zhihao Liu, Chunmei Xu, Di Zhang, Dewen Tong |
| Title | Circular RNA circPSAP functions as an efficient miR-331-3p sponge to regulate proliferation, apoptosis and bortezomib sensitivity of human multiple myeloma cells by upregulating HDAC4 |
| Journal | Journal of Pharmacological Sciences. 2022. |
| Authors | Hongyan Ma, Liyun Shen, Hua Yang, Hongtao Gong, Xingjun Du |
| Title | LINC00885 promotes cervical cancer progression through sponging miR-3150b-3p and upregulating BAZ2A |
| Journal | Biology Direct volume. 2022. |
| Authors | Yeling Liu, Jingrui Chen, Lizhong Zhou & Chunhua Yin |
| Title | Circular RNA FLNA acts as a sponge of miR-486-3p in promoting lung cancer progression via regulating XRCC1 and CYP1A1 |
| Journal | Cancer Gene Therapy. 2022. |
| Authors | Jiongwei Pan, Gang Huang, Zhangyong Yin, Xiaoping Cai, Enhui Gong, Yuling Li, Cunlai Xu, Zaiting Ye, Zhuo Cao & Wei Cheng |
| Title | Downregulation of Inflammatory Response via Nrf2/Trx1/TXNIP Axis in Oxidative Stress-Induced ARPE-19 Cells and Mouse Model of AMD |
| Journal | Oxidative Medicine and Cellular Longevity. 2022. |
| Authors | Qian Yang, Wenting Cai, Huizi Jin, Tianyi Shen and Jing Yu |
| Title | HLA complex P5 upregulation is correlated with poor prognosis and tumor progression in esophageal squamous cell carcinoma |
| Journal | Bioengineered. 2022. |
| Authors | Yaqin Wang, Zhijun Yu, Weidong Shi, Jian Shen, Yun Guan & Feng Ni |
| Title | Hsa_circ_0039569 facilitates the progression of endometrial carcinoma by targeting the miR-197/high mobility group protein A1 axis |
| Journal | Bioengineered. 2022. |
| Authors | Yi Zhoua Third, Anyi Pan, Yudong Zhang & Xinchun Li |
| Title | ALKBH5-mediated m6A modification of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription |
| Journal | Journal of Experimental & Clinical Cancer Research. 2022. |
| Authors | Leilei Liang, Yunshu Zhu, Jian Li, Jia Zeng & Lingying Wu |
| Title | CircATIC inhibits esophageal carcinoma progression and promotes radiosensitivity by elevating RHCG through sponging miR-10-3p |
| Journal | Thoracic Cancer. 2022. |
| Authors | Kai Zhang, Ruitai Fan, Deyao Zhao, Peipei Liu, Zheng Yang, Junqi Liu, Song Zhang, Shilei Rao, Yang Wang, Lixin Wan |
| Title | Immune-mediated neurodegenerative trait provoked by multimodal derepression of long-interspersed nuclear element-1 |
| Journal | iScience. 2022. |
| Authors | Fumio Takahashi, Chenyang Zhang, Hirohiko Hohjoh, Ben Raveney, Takashi Yamamura, Nobuhiro Hayashi, Shinji Oki |
| Title | Long non-coding RNA ZNF674-AS1 antagonizes oxaliplatin resistance of gastric cancer via regulating EZH2-mediated methylation of CHST7 |
| Journal | Aging (Albany NY). 2022. |
| Authors | Kun Ye and Yong Wang |
| Title | LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii |
| Journal | Aquaculture Reports. 2022. |
| Authors | Hua Zhang, Gaoyou Yao, Maoxian He |
| Title | An antagonistic pleiotropic gene regulates the reproductionand longevity tradeoff |
| Journal | PNAS. 2022. |
| Authors | Dou Wu, Zi Wang, Jingying Huang, Liang Huangi, Songbo Zhang, Ruixue Zhao, Wei Li, Di Chen, and Guangshuo Ou |
| Title | Long noncoding RNA LINC00930 promotes PFKFB3-mediated tumor glycolysis and cell proliferation in nasopharyngeal carcinoma |
| Journal | Journal of Experimental & Clinical Cancer Research. 2022. |
| Authors | Baoyu He, Hongli Pan, Fengque Zheng, Saiqiong Chen, Qingli Bie, Jinghe Cao, Rou Zhao, Jing Liang, Li Wei, Jianchao Zeng, Hui Li, Xing Cui, Yixuan Ding, et all. |
| Title | Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve |
| Journal | PLOS GENETICS. 2022. |
| Authors | Arnaud Chignon, Déborah Argaud, Marie-Chloé Boulanger, Ghada Mkannez, Valentin Bon-Baret, Zhonglin Li, Sébastien Thériault, Yohan Bossé, Patrick Mathieu |
| Title | Long non-coding RNA EVADR induced by Fusobacterium nucleatum infection promotes colorectal cancer metastasis |
| Journal | Cell Reports. 2022. |
| Authors | Xiaoxue,Lu, Qiaolin Xu, Yanan Tong, Zhujun Zhang, Guodong Dun, Yuyang Feng, Jie Tang, Dan Han, Yilan Mao, Ling Deng, Xiaoyi He, Qian Li, Yang Xiang, FengChao Wang, Dongzhu Zeng, Bin Tang, Xuhu Mao |
| Title | LncRNA-84277 is involved in chronic pain-related depressive behaviors through miR-128-3p/SIRT1 axis in central amygdala |
| Journal | Front Mol Neurosci. 2022. |
| Authors | Xiaowei Jiao, Ruiyao Wang, Xiaobao Ding, Binbin Yan, Yuwen Lin, Qiang Liu, Yuqing Wu, and Chenghua Zhou |
| Title | The Circular RNA CircCOL1A1 Functions as a miR-149-5p Sponge to Regulate the Formation of Superior-Quality Brush Hair via the CMTM3/AR Axis |
| Journal | Frontiers in Cell and Developmental Biology. 2022. |
| Authors | Wang J, Wu X, Sun X, Zhang L, Wang Q, Qu J, Wang Y, Li Y |
| Title | High-throughput analysis of ANRIL circRNA isoforms in human pancreatic islets |
| Journal | Scientific Reports. 2022. |
| Authors | Hannah J. MacMillan, Yahui Kong, Ezequiel Calvo-Roitberg, Laura C. Alonso & Athma A. Pai |
| Title | Hsa_circ_0048674 facilitates hepatocellular carcinoma progression and natural killer cell exhaustion depending on the regulation of miR-223-3p/PDL1 |
| Journal | History And Histopathology. 2022 |
| Authors | Suihui Li, Zhuangzhong Chen, Ruisheng Zhou, Sisi Wang, Wenping Wang, De Liu, Mengquan Li, Tiansheng Guo |
| Title | Overexpressed Thrombospondin 2 Induced Osteogenic Differentiation of Valve Interstitial Cells via Inhibition of Akt/ NF-κB Signaling Pathway to Promote Calcific Aortic Valve Disease Development |
| Journal | Hindawi. 2022 |
| Authors | Cheng Yu, Danna Wu, Chong Zhao, and Chaoguang Wu |
| Title | CircOAS3 regulates proliferation and psoriatic inflammation by interacting with Hsc70 via JNK/STAT3/NF-κB signaling pathways in keratinocytes |
| Journal | Research Square. 2022 |
| Authors | Zhenxian Yang, Xiran Yin, Cheng Chen, Shan Huang, Xueqing Li , Jianjun Yan, Qing Sun |
| Title | Circular RNA circ_SKA3 enhances gastric cancer development by targeting miR-520h |
| Journal | Histology and Histopathology. 2022 |
| Authors | Chuntao Wang, Hao Jiang, Jiaqun Peng, Duanshun Weng, Yu Zhang , Yanxun Zhou, Qin Zhang |
| Title | MicroRNA-mediated vascular intercellular communication is altered in chronic kidney disease |
| Journal | Cardiovascular Research. 2022 |
| Authors | Andreas Zietzer, Eva Steffen, Sven Niepmann, Philip Düsing, Mohammed Rabiul Hosen, Weiyi Liu, Paul Jamme, Baravan Al-Kassou, Philipp Roger Goody, Sebastian Zimmer, Katrin S Reiners, Alexander Pfeifer, Michael Böhm, Nikos Werner, Georg Nickenig, Felix Jansen |
| Title | Deoxyelephantopin Suppresses Pancreatic Cancer Progression In Vitro and In Vivo by Targeting linc00511/miR-370-5p/p21 Promoter Axis |
| Journal | Journal of Oncology. 2022 |
| Authors | Daolin Ji, Li Hou, Chunyang Xie, Haonan Feng, Dongdong Bao, Yue Teng, Junhao Liu, Tiangang Cui, Xiuhong Wang, Yi Xu, and Gang Tan |
| Title | Interleukin-22 Ameliorates Dextran Sulfate Sodium-Induced Colitis through the Upregulation of lncRNA-UCL to Accelerate Claudin-1 Expression via Sequestering miR-568 in Mice |
| Journal | Oxidative Medicine and Cellular Longevity. 2022 |
| Authors | Chonghua He, Zehan Chen, Jialan Huang, Riyun Gan, Jianyao Wang, Lisheng Wang, Defeng Li, and Jun Yao |
| Title | Circ_0078767 Inhibits the Progression of Non-Small-Cell Lung Cancer by Regulating the GPX3 Expression by Adsorbing miR-665 |
| Journal | International Journal of Genomics. 2022 |
| Authors | Xiting Liu, Ze Chen, Yuqiang Wu, Feng Gu, Dong Yan, Lei Yang, Qin Ma, and Caihong Fu |
| Title | Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells |
| Journal | Cell Death & Disease. 2022 |
| Authors | Hsing-Hua Chao, Jun-Liang Luo, Ming-Hsuan Hsu, Li-Han Chen, Tzu-Pin Lu, Mong-Hsun Tsai, Eric Y. Chuang, Li-Ling Chuang & Liang-Chuan Lai |
| Title | Tobacco Nicotine Promote TRAIL Resistance in Lung Cancer by SNHG5 |
| Journal | Research Square. 2022 |
| Authors | Xin Xiao, Juan Xu, Xiaoan Sheng, Chao Wang, Zhou Sun, Juanjuan Dong, and Xianfeng Shi |
| Title | Long non-coding RNA MAGI2-AS3 inactivates STAT3 pathway to inhibit prostate cancer cell proliferation via acting as a microRNA-424-5p sponge |
| Journal | J Cancer. 2022 |
| Authors | Xin Wei, Yi Hou, Yan Zhang, Huaiwei Zhang, Zhou Sun, Xiangdi Meng, and Zhixin Wang |
| Title | Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling |
| Journal | World J Gastrointest Oncol. 2022 |
| Authors | Wei Zheng, Guo-Liang Shen, Ke-Yang Xu, Qiao-Qiao Yin, Tian-Chen Hui, Zhe-Wen Zhou, Cheng-An Xu, Shou-Hao Wang, Wen-Hao Wu, Ling-Fei Shi, and Hong-Ying Pan |
| Title | Long non‑coding RNA LINC01748 exerts carcinogenic effects in non‑small cell lung cancer cell lines by regulating the microRNA‑520a‑5p/HMGA1 axis |
| Journal | International Journal of Molecular Medicine. 2021. |
| Authors | Yinling Tan, Fengxia Xu, Lingling Xu, Jianying Cui |
| Title | Long noncoding RNA LINC01291 promotes the aggressive properties of melanoma by functioning as a competing endogenous RNA for microRNA-625-5p and subsequently increasing IGF-1R expression |
| Journal | Cancer Gene Therapy. 2021. |
| Authors | Lijun Wu, Ke Li, Wei Lin, Jianjiang Liu, Qiang Qi, Guoliang Shen, Weixin Chen & Wenjun He |
| Title | F-circEA1 regulates cell proliferation and apoptosis through ALK downstream signaling pathway in non-small cell lung cancer |
| Journal | Human Cell. 2021. |
| Authors | Yinping Huo, Tangfeng Lv, Mingxiang Ye, Suhua Zhu, Jiaxin Liu, Hongbing Liu & Yong Song |
| Title | E2F transcription factor 1/small nucleolar RNA host gene 18/microRNA-338-5p/forkhead box D1: an important regulatory axis in glioma progression |
| Journal | Bioengineered. 2021 |
| Authors | Quanfeng Ma, Tianhao Yang |
| Title | C-MYC-induced upregulation of lncRNA SNHG12 regulates cell proliferation, apoptosis and migration in triple-negative breast cancer |
| Journal | Am J Transl Res. 2017 |
| Authors | Ouchen Wang, Fan Yang, Yehuan Liu, Lin Lv, Ruimin Ma, Chuanzhi Chen, Jiao Wang, Qiufan Tan,Yue Cheng, Erjie Xia, Yizuo Chen, Xiaohua Zhang |
| Title | Long noncoding RNA MHENCR promotes melanoma progression via regulating miR-425/489-mediated PI3K-Akt pathway |
| Journal | American Journal of Translational Research. 2017. |
| Authors | Chen X, Dong H, Liu S, Yu L, Yan D, Yao X, Sun W, Han D, Gao G. |
| Title | Neuronal hemoglobin affects dopaminergic cells’ response to stress |
| Journal | Cell Death and Disease. 2017. |
| Authors | Codrich M, Bertuzzi M, Russo R, Francescatto M, Espinoza S, Zentilin L, Giacca M, Cesselli D, Beltrami AP, Ascenzi P, Zucchelli S,, Persichetti F, Leanza G, Gustincich S |
| Title | Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1 |
| Journal | Nature Communications. 2016. |
| Authors | Kishikawa T, Otsuka M,, Yoshikawa T, Ohno M, Ijichi H, Koike K. |
| Title | Extracellular Vesicles Including Exosomes Regulate Innate Immune Responses to Hepatitis B Virus Infection |
| Journal | Frontiers in Immunology. 2016. |
| Authors | Kouwaki T, Fukushima Y, Daito T, Sanada T, Yamamoto N, Mifsud EJ, Leong CR, Tsukiyama-Kohara K, Kohara M, Matsumoto M, Seya T, Oshiumi H |
| Title | Evidence for a role of a lncRNA encoded from the p53 tumor suppressor gene in maintaining the undifferentiated state of human myeloid leukemias |
| Journal | Gene Reports. 2016. |
| Authors | Reisman, D., Gibson, A., Patel, M., & Wang, Y |
| Title | The microRNA cluster miR-183/96/182 contributes to long-term memory in a protein phosphatase 1-dependent manner |
| Journal | Nature Communications. 2016. |
| Authors | Woldemichael BT, Jawaid A, Kremer EA, Gaur N, Krol J, Marchais A, Mansuy IM |
| Title | Long noncoding RNA GPC3-AS1 promotes hepatocellular carcinoma progression via epigenetically activating GPC3 |
| Journal | The FEBS Journal. 2016. |
| Authors | Zhu XT, Yuan JH, Zhu TT, Li YY, Cheng XY |
| Title | Global transcript structure resolution of high gene density genomes through multi-platform data integration |
| Journal | Nucleic Acids Research. 2016. |
| Authors | O'Grady T, Wang X, Höner Zu Bentrup K, Baddoo M, Concha M, Flemington EK |
| Title | Long noncoding RNA GIHCG promotes hepatocellular carcinoma progression through epigenetically regulating miR-200b/a/429 |
| Journal | Journal of Molecular Medicine. 2016. |
| Authors | Sui CJ, Zhou YM, Shen WF, Dai BH, Lu JJ, Zhang MF, Yang JM |
| Title | miR-34 activity is modulated through 5′-end phosphorylation in response to DNA damage |
| Journal | Nature Communications. 2016. |
| Authors | Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack FJ, Weidhaas JB |
| Title | Long noncoding RNA FGFR3-AS1 promotes osteosarcoma growth through regulating its natural antisense transcript FGFR3 |
| Journal | Molecular Biology Reports. 2016. |
| Authors | Sun, J., Wang, X., Fu, C., Wang, X., Zou, J., Hua, H., & Bi, Z. |
| Title | Genetic predisposition to neuroblastoma mediated by a LMO1 super-enhancer polymorphism |
| Journal | Nature. 2015. |
| Authors | Derek A. Oldridge, Andrew C. Wood, Nina Weichert-Leahey, Ian Crimmins, Robyn Sussman, Cynthia Winter, Lee D. McDaniel, Maura Diamond, Lori S. Hart, Shizhen Zhu, Adam D. Durbin, Brian J. Abraham, Lars Anders, Lifeng Tian, Shile Zhang, Jun S. Wei, Javed Khan, Kelli Bramlett, Nazneen Rahman, Mario Capasso, Achille Iolascon, Daniela S. Gerhard, Jaime M. Guidry Auvil, Richard A. Young, Hakon Hakonarson et al. |
| Title | Long Non-coding RNA Growth Arrest-specific Transcript 5 (GAS5) Inhibits Liver Fibrogenesis through a Mechanism of Competing Endogenous RNA |
| Journal | The Journal of Biological Chemistry. 2015. |
| Authors | Fujun Yu, Jianjian Zheng, Yuqing Mao, Peihong Dong, Zhongqiu Lu, Guojun Li, Chuanyong Guo, Zhanju Liu and Xiaoming Fan |
| Title | The Role of Long Intergenic Noncoding RNA p21 in Vascular Endothelial Cells |
| Journal | DNA and Cell Biology. 2015. |
| Authors | He C, Ding JW, Li S, Wu H, Jiang YR, Yang W, Teng L, Yang J, Yang J |
| Title | Regulation of p53 during senescence in normal human keratinocytes |
| Journal | Aging Cell. 2015. |
| Authors | Kim RH, Kang MK, Kim T, Yang P, Bae S, Williams DW, Phung S, Shin KH, Hong C, Park NH |
| Title | New non-coding lytic transcripts derived from the Epstein Barr virus latency origin of replication oriP are hyper-edited, bind the paraspeckle protein, NONO/p54nrb, and support lytic viral transcription. |
| Journal | Journal of Virology. 2015. |
| Authors | Cao S, Moss W, O'Grady T, Concha M, Strong MJ, Wang X, Yu Y, Baddoo M, Zhang K, Fewell C, Lin Z, Dong Y, Flemington EK |
| Title | A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature |
| Journal | Nature Structural & Molecular Biology. 2015. |
| Authors | Inma Gonzalez, Roberto Munita, Eneritz Agirre, Travis A Dittmer, Katia Gysling, Tom Misteli & Reini F Luco |
| Title | Microtubule disruption synergizes with oncolytic virotherapy by inhibiting interferon translation and potentiating bystander killing. |
| Journal | Nature Communications. 2015. |
| Authors | Rozanne Arulanandam, Cory Batenchuk, Oliver Varette, Chadi Zakaria, Vanessa Garcia, Nicole E. Forbes, Colin Davis, Ramya Krishnan, Raunak Karmacharya, Julie Cox, Anisha Sinha, Andrew Babawy, KatherineWaite, EricaWeinstein, Theresa Falls, Andrew Chen, Jeff Hamill, Naomi De Silva, David P. Conrad, Harold Atkins, Kenneth Garson, Carolina Ilkow, Mads Kærn, Barbara Vanderhyden, Nahum Sonenberg, Tommy Alain, Fabrice Le Boeuf, John C. Bell1, & Jean-Simon Diallo |
| Title | SP1-induced upregulation of the long noncoding RNA TINCR regulates cell proliferation and apoptosis by affecting KLF2 mRNA stability in gastric cancer. |
| Journal | Oncogene. 2015. |
| Authors | Xu TP, Liu XX, Xia R, Yin L, Kong R, Chen WM, Huang MD, Shu YQ. |
| Title | Developmental regulation of human cortex transcription and its clinical relevance at single base resolution. |
| Journal | Nature Neuroscience. 2014. |
| Authors | Andrew E Jaffe, Jooheon Shin, Leonardo Collado-Torres, Jeffrey T Leek, Ran Tao, Chao Li, Yuan Gao, Yankai Jia, Brady J Maher, Thomas M Hyde, Joel E Kleinman & Daniel R Weinberger. |
| Title | High-Throughput RNA sequencing based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project. |
| Journal | Journal of Virology. 2014. |
| Authors | Cao S, Strong MJ, Wang X, Moss WN, Concha M, Lin Z, O'Grady T, Baddoo M, Fewell C, Renne R, Flemington EK. |
| Title | Long Noncoding RNA-EBIC Promotes Tumor Cell Invasion by Binding to EZH2 and Repressing E-Cadherin in Cervical Cancer. |
| Journal | PLoS ONE. 2014. |
| Authors | Ning-xia Sun, Chen Ye, Qian Zhao, Qing Zhang, Chen Xu, Shao-bing Wang, Zhi-jun Jin, Shu-han Sun, Fang Wang, Wen Li mail |
| Title | Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene. |
| Journal | Molecular Biotechnology. 2014. |
| Authors | Sakaguchi M, Watanabe M, Kinoshita R, Kaku H, Ueki H, Futami J, Murata H, Inoue Y, Li SA, Huang P, Putranto EW, Ruma IM, Nasu Y, Kumon H, Huh NH. |
| Title | Strain-level genomic variation in natural populations of Lebetimonas from an erupting deep-sea volcano. |
| Journal | Journal of Virology. 2013. |
| Authors | O'Grady T, Cao S, Strong MJ, Concha M, Wang X, Bondurant SS, Adams M, Baddoo M, Srivastav SK, Lin Z, Fewell C, Yin Q, Flemington EK. |
| Title | Efficient cellular fractionation improves RNA sequencing analysis of mature and nascent transcripts from human tissues. |
| Journal | BMC Biotechnology. 2013. |
| Authors | Zaghlool A, Ameur A, Nyberg L, Halvardson J, Grabherr M, Cavelier L, Feuk L. |
| Title | Dengue virus subgenomic RNA induces apoptosis through the Bcl-2-mediated PI3k/Akt signaling pathway. |
| Journal | Virology. 2013. |
| Authors | Liua Y, Liua H, Zoua J, Zhanga B and Yuana Z |
| Title | Identification of a long non-coding RNA-associated RNP complex regulating metastasis at the translational step |
| Journal | The EMBO Journal. 2013. |
| Authors | Gumireddy K, Li A, Yan J, Setoyama T, Johannes GJ, Orom UA, Tchou J, Liu Q, Zhang L, Speicher DW, Calin GA, Huang Q. |
| Title | MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability. |
| Journal | Nucleic Acids Research. 2013. |
| Authors | Li J, Chen Y, Qin X, Wen J, Ding H, Xia W, Li S, Su X, Wang W, Li H, Zhao Q, Fang T, Qu L, Shao N. |
| Title | Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus. |
| Journal | Proceedings of the National Academy of Sciences of the United States of America. 2013. |
| Authors | Lee H, Komano J, Saitoh Y, Yamaoka S, Kozaki T, Misawa T, Takahama M, Satoh T, Takeuchi O, Yamamoto N, Matsuura Y, Saitoh T, Akira S. |
| Title | DNA-Damage-Induced Nuclear Export of Precursor MicroRNAs Is Regulated by the ATM-AKT Pathway. |
| Journal | Cell. 2013. |
| Authors | Wan G,Zhang X, Langley R, Liu Y, Hu X, Han C, Peng G, Ellis L, Jones S, Lu X |
| Title | Btf and TRAP150 have distinct roles in regulating subcellular mRNA distribution. |
| Journal | Nucleus. 2013. |
| Authors | Varia S, Potabathula D, Deng Z, Bubulya A, Bubulya PA. |
| Title | Nuclear Phosphoinositide-Specific Phospholipase C β1 Controls Cytoplasmic CCL2 mRNA Levels in HIV-1 gp120-Stimulated Primary Human Macrophages. |
| Journal | PLoS One. 2013. |
| Authors | Spadaro F, Cecchetti S, Purificato C, Sabbatucci M, Podo F, Ramoni C, Gessani S, Fantuzzi L. |
| Title | Circular RNAs are abundant, conserved, and associated with ALU repeats. |
| Journal | RNA: A Publication of the RNA Society. 2013. |
| Authors | Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF, Sharpless NE. |
| Title | Long non-coding antisense RNA controls Uchl1 translation through an embedded SINEB2 repeat. |
| Journal | Nature. 2012. |
| Authors | Carrieri C, Cimatti L, Biagioli M, Beugnet A, Zucchelli S, Fedele S, Pesce E, Ferrer I, Collavin L, Santoro C, Forrest AR, Carninci P, Biffo S, Stupka E, Gustincich S. |
| Title | Characterization of Novel Paternal ncRNAs at the Plagl1 Locus, Including Hymai, Predicted to Interact with Regulators of Active Chromatin. |
| Journal | PLoS One. 2012. |
| Authors | Iglesias-Platas I, Martin-Trujillo A, Cirillo D, Court F, Guillaumet-Adkins A, Camprubi C, Bourc'his D, Hata K, Feil R, Tartaglia G, Arnaud P, Monk D. |
| Title | MxA inhibits hepatitis B virus replication by interaction with core protein HBcAg. |
| Journal | Hepatology. 2012. |
| Authors | Li N, Zhang L, Chen L, Feng W, Xu Y, Chen F, Liu X, Chen Z, Liu W. |
| Title | Reduced DICER1 elicits an interferon response in endometrial cancer cells. |
| Journal | Molecular Cancer Research. 2012. |
| Authors | Chiappinelli KB, Haynes BC, Brent MR, Goodfellow PJ. |
| Title | Monoallelic Expression Determines Oncogenic Progression and Outcome in Benign and Malignant Brain Tumors. |
| Journal | Cancer Research. 2011. |
| Authors | Walker EJ, Zhang C, Castelo-Branco P, Hawkins C, Wilson W, Zhukova N, Alon N, Novokmet A, Baskin B, Ray P, Knobbe C, Dirks P, Taylor MD, Croul S, Malkin D, Tabori U. |
| Title | Identification of new viral genes and transcript isoforms during Epstein-Barr virus reactivation using RNA-seq. |
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| Title | Small Noncoding RNA Modulates Japanese Encephalitis Virus Replication and Translation in trans. |
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| Title | Strand antagonism in RNAi: an explanation of differences in potency between intracellularly expressed siRNA and shRNA. |
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Cytoplasmic and Nuclear RNA Purification Kit
This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.
Background
In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.
RNA Purification Tutorial - Cytoplasmic and Nuclear RNA
Supporting Data
Figure 1. Effective Separation of HeLa Cell Cytoplasmic & Nuclear RNA. Norgen's Cytoplasmic & Nuclear RNA Purification Kit was used to effectively separate cytoplasmic and nuclear RNA from 0.75 million HeLa cells in triplicate. Panel A: High quality cytoplasmic and nuclear RNA was purified using Norgen's kit. Note the integrity and quality of both cytoplasmic and nuclear RNA. Ten microliters of each 50 µL elution were run on a 1.5% formaldehyde-agarose gel. Lane M is Norgen's 1 kb RNA Ladder, lanes 1-3 contain nuclear RNA and lanes 4-6 contain cytoplasmic RNA. Panel B: Ten microliters of the above cytoplasmic and nuclear RNA isolated from HeLa cells using Norgen's kit was run on a 0.9% agarose DNA gel. Genomic DNA is clearly visible in the nuclear RNA fractions (lanes 1-3), however, no genomic DNA can be detected in the cytoplasmic RNA fractions (lanes 4-6). Note that an optional on-column DNase treatment protocol is provided to remove the genomic DNA in the nuclear fraction.
Figure 2. Superior Separation of HeLa Cell Cytoplasmic & Nuclear RNA. Norgen's Cytoplasmic & Nuclear RNA Purification Kit provides better separation of cytoplasmic and nuclear RNA from 0.8 million HeLa cells when compared to a leading competitors product. Panel A: Cytoplasmic and nuclear RNA purified from HeLa cells using Norgen's kit and a competitor's kit. Ten microliters of each 50 µL elution (Norgen or competitor's kit) of the cytoplasmic (C) or nuclear (N) RNA were run on a 1.5% formaldehyde-agarose gel. Higher yields of RNA with good integrity were isolated using Norgen's kit. Panel B: Ten microliters of the cytoplasmic and nuclear RNA isolated from HeLa cells using Norgen's kit and the competitor's kit was run on a 0.9% agarose gel. Genomic DNA only co-migrates with the nuclear fraction in RNA isolated using Norgens Cytoplasmic & Nuclear RNA Purification Kit, not the cytoplasmic fraction. Note that an optional on-column DNase treatment protocol is provided to remove the genomic DNA in the nuclear fraction. In contrast, significant genomic DNA contamination was observed in the cytoplasmic fraction of the RNA isolated using the competitor's kit.
Figure 3. Genomic DNA-free Cytoplasmic RNA. RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Norgen's Cytoplasmic & Nuclear RNA Purification Kit. Lane M is Norgen's PCR Sizer DNA ladder, Lanes 1 and 3 are the negative control (PCR only, without reverse transcript), and Lanes 2 and 4 are the actual RT-PCR that show the expected 166 bp RT-PCR product. The expected amplicon size from the gene copy is the same as that from the RNA transcript. The lack of product in lanes 1 and 3 indicates that no genomic DNA contamination is present in the isolated cytoplasmic RNA. All PCR products were resolved on a 1X TAE, 2% agarose gel.
Figure 4. Specific Separation and Subsequent Amplification of Cytoplasmic and Nuclear Transcripts. Norgen's Cytoplasmic & Nuclear RNA Purification Kit effectively separates cytoplasmic and nuclear RNA. RT-PCR was performed using human U2 snRNA (Panel A) or S14 (Panel B) primers on 0.5 µg of either cytoplasmic or nuclear RNA fractions isolated from 1 million HeLa cells using Norgen's Cytoplasmic & Nuclear RNA Purification Kit. In Panel A, an intense PCR product was observed only in the nuclear fraction when the nuclear-specific U2 snRNA primers were used. In Panel B, the majority of the PCR product for the house-keeping transcript of S14 was observed in the cytoplasmic fraction. The two results combined showed effective separation of the cytoplasmic and nuclear RNA using Norgen's Cytoplasmic & Nuclear RNA Purification Kit. All PCR products were resolved on a 1X TAE, 1.5% agarose DNA gel.
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Kit Specifications
You have selected: Cat. 21000
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
Up to 50 µg RNA
|
|
Maximum Column Loading Volume
|
650 µL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt)
|
| Time to Complete 10 Purifications |
45 minutes
|
|
RNA Yield
HeLa (1 x 106) - Cytoplasmic RNA HeLa (1 x 106) - Nuclear RNA |
15 µg ≤ 3.5 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
You have selected: Cat. 37400
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
Up to 50 µg RNA
|
|
Maximum Column Loading Volume
|
650 µL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt)
|
| Time to Complete 10 Purifications |
45 minutes
|
|
RNA Yield
HeLa (1 x 106) - Cytoplasmic RNA HeLa (1 x 106) - Nuclear RNA |
15 µg ≤ 3.5 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 21000 (50 preps) | Cat. 37400 (100 preps) |
|---|---|---|
| Lysis Buffer J | 20 mL | 2 x 20 mL |
| Buffer SK | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer E | 6 mL | 2 x 6 mL |
| Mini Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
Protocols:
Application Notes:
Posters:
Safety Data Sheets:
| Title | CircESRP1 enhances metastasis and epithelial–mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis |
| Journal | Journal of Translational Medicine. 2022. |
| Authors | Rui Shi, Wei Zhang, Jun Zhang, Zhicheng Yu, Lanfen An, Rong Zhao, Xing Zhou, Ziwei Wang, Sitian Wei & Hongbo Wang |
| Title | Identification of a novel circular RNA circZNF652/miR-486-5p/SERPINE1 signaling cascade that regulates cancer aggressiveness in glioblastoma (GBM) |
| Journal | Bioengineered. 2022. |
| Authors | Liang Liu, Shan Xiao, Yan Wang, Zifeng Zhu, Yiyao Cao, Sen Yang, Rongkang Mai &Yong Zheng |
| Title | A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis |
| Journal | Cell Death & Disease. 2022. |
| Authors | Yuan Tan, Chonghua Jiang, Qunying Jia, Jing Wang, Ge Huang & Faqing Tang |
| Title | Long non‑coding RNA LINC00238 suppresses the malignant phenotype of liver cancer by sponging miR‑522 |
| Journal | Molecular Medicine Reports. 2022. |
| Authors | Hong-Gang Qian, Qiong Wu, Jian-Hui Wu, Xiu-Yun Tian, Wei Xu, Chun-Yi Hao |
| Title | Coenzyme Q regulates UCP1 expression and thermogenesis through the integrated stress responses |
| Journal | bioRxiv. 2022. |
| Authors | Ching-Fang Chang, Amanda L. Gunawan, Peter-James H. Zushin, Greg A. Timblin, Ambre M. Bertholet, Biao Wang, Kaoru Sajio, Yuriy Kirichok, Andreas Stahl |
| Title | circKLHL24 Blocks Breast Cancer Development by Regulating the miR-1204/ALX4 Network |
| Journal | Cancer Biotherapy & Radiopharmaceuticals. 2022. |
| Authors | Guangming Yi, Dan Wang, Jianjun Han, Li Jia, Xiaojun Liu, and Jun He |
| Title | RBM47/SNHG5/FOXO3 axis activates autophagy and inhibits cell proliferation in papillary thyroid carcinoma |
| Journal | Cell Death & Disease. 2022. |
| Authors | Yuan Qin, Wei Sun, Zhihong Wang, Wenwu Dong, Liang He, Ting Zhang, Chengzhou Lv & Hao Zhang |
| Title | CircUCK2 regulates HECTD1-mediated endothelial-mesenchymal transition inhibition by interacting with FUS and protects the blood–brain barrier in ischemic stroke |
| Journal | The kaohsiung Journal of Medical Sciences. 2022. |
| Authors | Jun-Hong Zhuang, Huan-Xiong Chen, Ning Gao, Rong-Dao Sun, Cheng-Ye Xiao, De-Hua Zeng, Zheng-Tao Yu, Jun Peng, Ying Xia |
| Title | Long non-coding RNA muskelin 1 antisense RNA as a potential therapeutic target in hepatocellular carcinoma treatment |
| Journal | Bioengineered . 2022. |
| Authors | Xijun Chen, Qing Ye, Zhigao Chen, Qian Lin, Wen Chen, Chengrong Xie & Xiaomin Wang |
| Title | Long non-coding RNA ADAMTS9-AS1 attenuates ferroptosis by Targeting microRNA-587/solute carrier family 7 member 11 axis in epithelial ovarian cancer |
| Journal | Bioengineered . 2022. |
| Authors | Li Cai, Xiaoqing Hu, Lu Ye, Pingjuan Bai, Youkun Jie & Kuanyong Shu |
| Title | A new circular RNA–encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)–induced mitochondrial dysfunction |
| Journal | JBC. 2022. |
| Authors | Xiaomin Zhao, Jianxiong Guo, Xinyue Wang, Jiadi Lin, Zhihao Liu, Chunmei Xu, Di Zhang, Dewen Tong |
| Title | Circular RNA circPSAP functions as an efficient miR-331-3p sponge to regulate proliferation, apoptosis and bortezomib sensitivity of human multiple myeloma cells by upregulating HDAC4 |
| Journal | Journal of Pharmacological Sciences. 2022. |
| Authors | Hongyan Ma, Liyun Shen, Hua Yang, Hongtao Gong, Xingjun Du |
| Title | LINC00885 promotes cervical cancer progression through sponging miR-3150b-3p and upregulating BAZ2A |
| Journal | Biology Direct volume. 2022. |
| Authors | Yeling Liu, Jingrui Chen, Lizhong Zhou & Chunhua Yin |
| Title | Circular RNA FLNA acts as a sponge of miR-486-3p in promoting lung cancer progression via regulating XRCC1 and CYP1A1 |
| Journal | Cancer Gene Therapy. 2022. |
| Authors | Jiongwei Pan, Gang Huang, Zhangyong Yin, Xiaoping Cai, Enhui Gong, Yuling Li, Cunlai Xu, Zaiting Ye, Zhuo Cao & Wei Cheng |
| Title | Downregulation of Inflammatory Response via Nrf2/Trx1/TXNIP Axis in Oxidative Stress-Induced ARPE-19 Cells and Mouse Model of AMD |
| Journal | Oxidative Medicine and Cellular Longevity. 2022. |
| Authors | Qian Yang, Wenting Cai, Huizi Jin, Tianyi Shen and Jing Yu |
| Title | HLA complex P5 upregulation is correlated with poor prognosis and tumor progression in esophageal squamous cell carcinoma |
| Journal | Bioengineered. 2022. |
| Authors | Yaqin Wang, Zhijun Yu, Weidong Shi, Jian Shen, Yun Guan & Feng Ni |
| Title | Hsa_circ_0039569 facilitates the progression of endometrial carcinoma by targeting the miR-197/high mobility group protein A1 axis |
| Journal | Bioengineered. 2022. |
| Authors | Yi Zhoua Third, Anyi Pan, Yudong Zhang & Xinchun Li |
| Title | ALKBH5-mediated m6A modification of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription |
| Journal | Journal of Experimental & Clinical Cancer Research. 2022. |
| Authors | Leilei Liang, Yunshu Zhu, Jian Li, Jia Zeng & Lingying Wu |
| Title | CircATIC inhibits esophageal carcinoma progression and promotes radiosensitivity by elevating RHCG through sponging miR-10-3p |
| Journal | Thoracic Cancer. 2022. |
| Authors | Kai Zhang, Ruitai Fan, Deyao Zhao, Peipei Liu, Zheng Yang, Junqi Liu, Song Zhang, Shilei Rao, Yang Wang, Lixin Wan |
| Title | Immune-mediated neurodegenerative trait provoked by multimodal derepression of long-interspersed nuclear element-1 |
| Journal | iScience. 2022. |
| Authors | Fumio Takahashi, Chenyang Zhang, Hirohiko Hohjoh, Ben Raveney, Takashi Yamamura, Nobuhiro Hayashi, Shinji Oki |
| Title | Long non-coding RNA ZNF674-AS1 antagonizes oxaliplatin resistance of gastric cancer via regulating EZH2-mediated methylation of CHST7 |
| Journal | Aging (Albany NY). 2022. |
| Authors | Kun Ye and Yong Wang |
| Title | LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii |
| Journal | Aquaculture Reports. 2022. |
| Authors | Hua Zhang, Gaoyou Yao, Maoxian He |
| Title | An antagonistic pleiotropic gene regulates the reproductionand longevity tradeoff |
| Journal | PNAS. 2022. |
| Authors | Dou Wu, Zi Wang, Jingying Huang, Liang Huangi, Songbo Zhang, Ruixue Zhao, Wei Li, Di Chen, and Guangshuo Ou |
| Title | Long noncoding RNA LINC00930 promotes PFKFB3-mediated tumor glycolysis and cell proliferation in nasopharyngeal carcinoma |
| Journal | Journal of Experimental & Clinical Cancer Research. 2022. |
| Authors | Baoyu He, Hongli Pan, Fengque Zheng, Saiqiong Chen, Qingli Bie, Jinghe Cao, Rou Zhao, Jing Liang, Li Wei, Jianchao Zeng, Hui Li, Xing Cui, Yixuan Ding, et all. |
| Title | Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve |
| Journal | PLOS GENETICS. 2022. |
| Authors | Arnaud Chignon, Déborah Argaud, Marie-Chloé Boulanger, Ghada Mkannez, Valentin Bon-Baret, Zhonglin Li, Sébastien Thériault, Yohan Bossé, Patrick Mathieu |
| Title | Long non-coding RNA EVADR induced by Fusobacterium nucleatum infection promotes colorectal cancer metastasis |
| Journal | Cell Reports. 2022. |
| Authors | Xiaoxue,Lu, Qiaolin Xu, Yanan Tong, Zhujun Zhang, Guodong Dun, Yuyang Feng, Jie Tang, Dan Han, Yilan Mao, Ling Deng, Xiaoyi He, Qian Li, Yang Xiang, FengChao Wang, Dongzhu Zeng, Bin Tang, Xuhu Mao |
| Title | LncRNA-84277 is involved in chronic pain-related depressive behaviors through miR-128-3p/SIRT1 axis in central amygdala |
| Journal | Front Mol Neurosci. 2022. |
| Authors | Xiaowei Jiao, Ruiyao Wang, Xiaobao Ding, Binbin Yan, Yuwen Lin, Qiang Liu, Yuqing Wu, and Chenghua Zhou |
| Title | The Circular RNA CircCOL1A1 Functions as a miR-149-5p Sponge to Regulate the Formation of Superior-Quality Brush Hair via the CMTM3/AR Axis |
| Journal | Frontiers in Cell and Developmental Biology. 2022. |
| Authors | Wang J, Wu X, Sun X, Zhang L, Wang Q, Qu J, Wang Y, Li Y |
| Title | High-throughput analysis of ANRIL circRNA isoforms in human pancreatic islets |
| Journal | Scientific Reports. 2022. |
| Authors | Hannah J. MacMillan, Yahui Kong, Ezequiel Calvo-Roitberg, Laura C. Alonso & Athma A. Pai |
| Title | Hsa_circ_0048674 facilitates hepatocellular carcinoma progression and natural killer cell exhaustion depending on the regulation of miR-223-3p/PDL1 |
| Journal | History And Histopathology. 2022 |
| Authors | Suihui Li, Zhuangzhong Chen, Ruisheng Zhou, Sisi Wang, Wenping Wang, De Liu, Mengquan Li, Tiansheng Guo |
| Title | Overexpressed Thrombospondin 2 Induced Osteogenic Differentiation of Valve Interstitial Cells via Inhibition of Akt/ NF-κB Signaling Pathway to Promote Calcific Aortic Valve Disease Development |
| Journal | Hindawi. 2022 |
| Authors | Cheng Yu, Danna Wu, Chong Zhao, and Chaoguang Wu |
| Title | CircOAS3 regulates proliferation and psoriatic inflammation by interacting with Hsc70 via JNK/STAT3/NF-κB signaling pathways in keratinocytes |
| Journal | Research Square. 2022 |
| Authors | Zhenxian Yang, Xiran Yin, Cheng Chen, Shan Huang, Xueqing Li , Jianjun Yan, Qing Sun |
| Title | Circular RNA circ_SKA3 enhances gastric cancer development by targeting miR-520h |
| Journal | Histology and Histopathology. 2022 |
| Authors | Chuntao Wang, Hao Jiang, Jiaqun Peng, Duanshun Weng, Yu Zhang , Yanxun Zhou, Qin Zhang |
| Title | MicroRNA-mediated vascular intercellular communication is altered in chronic kidney disease |
| Journal | Cardiovascular Research. 2022 |
| Authors | Andreas Zietzer, Eva Steffen, Sven Niepmann, Philip Düsing, Mohammed Rabiul Hosen, Weiyi Liu, Paul Jamme, Baravan Al-Kassou, Philipp Roger Goody, Sebastian Zimmer, Katrin S Reiners, Alexander Pfeifer, Michael Böhm, Nikos Werner, Georg Nickenig, Felix Jansen |
| Title | Deoxyelephantopin Suppresses Pancreatic Cancer Progression In Vitro and In Vivo by Targeting linc00511/miR-370-5p/p21 Promoter Axis |
| Journal | Journal of Oncology. 2022 |
| Authors | Daolin Ji, Li Hou, Chunyang Xie, Haonan Feng, Dongdong Bao, Yue Teng, Junhao Liu, Tiangang Cui, Xiuhong Wang, Yi Xu, and Gang Tan |
| Title | Interleukin-22 Ameliorates Dextran Sulfate Sodium-Induced Colitis through the Upregulation of lncRNA-UCL to Accelerate Claudin-1 Expression via Sequestering miR-568 in Mice |
| Journal | Oxidative Medicine and Cellular Longevity. 2022 |
| Authors | Chonghua He, Zehan Chen, Jialan Huang, Riyun Gan, Jianyao Wang, Lisheng Wang, Defeng Li, and Jun Yao |
| Title | Circ_0078767 Inhibits the Progression of Non-Small-Cell Lung Cancer by Regulating the GPX3 Expression by Adsorbing miR-665 |
| Journal | International Journal of Genomics. 2022 |
| Authors | Xiting Liu, Ze Chen, Yuqiang Wu, Feng Gu, Dong Yan, Lei Yang, Qin Ma, and Caihong Fu |
| Title | Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells |
| Journal | Cell Death & Disease. 2022 |
| Authors | Hsing-Hua Chao, Jun-Liang Luo, Ming-Hsuan Hsu, Li-Han Chen, Tzu-Pin Lu, Mong-Hsun Tsai, Eric Y. Chuang, Li-Ling Chuang & Liang-Chuan Lai |
| Title | Tobacco Nicotine Promote TRAIL Resistance in Lung Cancer by SNHG5 |
| Journal | Research Square. 2022 |
| Authors | Xin Xiao, Juan Xu, Xiaoan Sheng, Chao Wang, Zhou Sun, Juanjuan Dong, and Xianfeng Shi |
| Title | Long non-coding RNA MAGI2-AS3 inactivates STAT3 pathway to inhibit prostate cancer cell proliferation via acting as a microRNA-424-5p sponge |
| Journal | J Cancer. 2022 |
| Authors | Xin Wei, Yi Hou, Yan Zhang, Huaiwei Zhang, Zhou Sun, Xiangdi Meng, and Zhixin Wang |
| Title | Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling |
| Journal | World J Gastrointest Oncol. 2022 |
| Authors | Wei Zheng, Guo-Liang Shen, Ke-Yang Xu, Qiao-Qiao Yin, Tian-Chen Hui, Zhe-Wen Zhou, Cheng-An Xu, Shou-Hao Wang, Wen-Hao Wu, Ling-Fei Shi, and Hong-Ying Pan |
| Title | Long non‑coding RNA LINC01748 exerts carcinogenic effects in non‑small cell lung cancer cell lines by regulating the microRNA‑520a‑5p/HMGA1 axis |
| Journal | International Journal of Molecular Medicine. 2021. |
| Authors | Yinling Tan, Fengxia Xu, Lingling Xu, Jianying Cui |
| Title | Long noncoding RNA LINC01291 promotes the aggressive properties of melanoma by functioning as a competing endogenous RNA for microRNA-625-5p and subsequently increasing IGF-1R expression |
| Journal | Cancer Gene Therapy. 2021. |
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| Title | F-circEA1 regulates cell proliferation and apoptosis through ALK downstream signaling pathway in non-small cell lung cancer |
| Journal | Human Cell. 2021. |
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| Title | E2F transcription factor 1/small nucleolar RNA host gene 18/microRNA-338-5p/forkhead box D1: an important regulatory axis in glioma progression |
| Journal | Bioengineered. 2021 |
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| Title | C-MYC-induced upregulation of lncRNA SNHG12 regulates cell proliferation, apoptosis and migration in triple-negative breast cancer |
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| Title | Long noncoding RNA MHENCR promotes melanoma progression via regulating miR-425/489-mediated PI3K-Akt pathway |
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| Title | Extracellular Vesicles Including Exosomes Regulate Innate Immune Responses to Hepatitis B Virus Infection |
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| Title | Evidence for a role of a lncRNA encoded from the p53 tumor suppressor gene in maintaining the undifferentiated state of human myeloid leukemias |
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| Title | Long Non-coding RNA Growth Arrest-specific Transcript 5 (GAS5) Inhibits Liver Fibrogenesis through a Mechanism of Competing Endogenous RNA |
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| Title | The Role of Long Intergenic Noncoding RNA p21 in Vascular Endothelial Cells |
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