Cytoplasmic and Nuclear RNA Purification Kit (Cat. 21000, 37400)

For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues

  • Excellent separation and purification of cytoplasmic and nuclear RNA
  • Convenient and fast spin column format
  • High quality and yield of RNA
  • Isolate full diversity of RNA (including microRNA) without phenol
  • Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
  • Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR

ItemsCat. #Size
Cytoplasmic & Nuclear RNA Purification Kit 21000 50 preps
Cytoplasmic & Nuclear RNA Purification Kit 37400 100 preps

Cytoplasmic and Nuclear RNA Purification Kit

This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.

Background

In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.

Kit Specifications
Binding Capacity Per Column
Up to 50 µg RNA
Maximum Loading Volume Per Spin Column
650 µL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
45 minutes
RNA Yield
HeLa (1 x 106) - Cytoplasmic RNA
HeLa (1 x 106) - Nuclear RNA
15 µg
≤ 3.5 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 21000 (50 preps) Cat. 37400 (100 preps)
Lysis Buffer J 20 mL 2 x 20 mL
Buffer SK 40 mL 2 x 40 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Buffer E 6 mL 2 x 6 mL
Mini Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1

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