• Detection kits for E.coli O157:H7
  • Available in 2 formats for analysis:
 

ItemsCat. #Size
E.coli O157:H7 TaqMan PCR Kit TM41300 24 rxns
E.coli O157:H7 TaqMan Probe/Primer and Control Set TM41310 100 rxns
E.coli O157:H7 End-Point PCR Kit EP41300 24 rxns
E.coli O157:H7 Primer and Control Set EP41310 100 rxns

E.coli O157:H7 Detection Kits

E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.

There are 2 kits available for the detection of E.coli O157:H7:

E.coli O157:H7 TaqMan PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
E.coli O157:H7 End-Point PCR Kit
  • Master Mix and primers for the specific amplification of a 348 bp and 587 bp nucleotide region of the E.coli O157:H7 genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM41300
(24 rxns)
End-Point
Cat. EP41300
(24 rxns)
MDx 2X PCR Master Mix - 350 µL
MDx TaqMan 2X PCR Master Mix 350 µL -
E.coli O157:H7 Primer Mix 70 µL 70 µL
E.coli O157:H7 Positive Control 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Loading Dye - 100 µL
DNA Ladder - 100 µL
Product Insert 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM41310
(100 rxns)
Primer
and Control Set
Cat. EP41310
(100 rxns)
E.coli O157:H7 Primer Mix 3 x 70 µL 3 x 70 µL
E.coli O157:H7 Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1