E.coli O157:H7 TaqMan PCR Detection Kits

Cat. TM41350, TM41310

E.coli O157:H7 Detection Kit (100 reactions)

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E.coli O157:H7 TaqMan PCR Detection Kits

Cat. TM41350, TM41310

Detection kits for E.coli O157:H7
Available in TaqMan format for analysis

For research use only and NOT intended for in vitro diagnostics.

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E.coli O157:H7 TaqMan PCR Kit
(Cat. TM41350) 100 rxns

E.coli O157:H7 TaqMan Probe/Primer and Control Set
(Cat. TM41310) 100 rxns

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. TM41350

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

You have selected: Cat. TM41310

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Supporting Data

TaqMan PCR Kit -Figure 1. Example of TaqMan PCR Positive result. Both PCR signals (FAM and Cy5) above the baseline from FAM, Cy5 and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 2. Example of TaqMan PCR Positive (Cy5) result. Both PCR signals above the baseline from Cy5 and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 3. Example of TaqMan PCR Positive (FAM) result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 4. Example of TaqMan PCR Negative result. No target DNA was detected in FAM and Cy5 channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit -Figure 5. Example of TaqMan PCR inhibition result. No signal from FAM, Cy5 and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

Click for expanded view

Protocols:

(TM41350) E.coli O157:H7 TaqMan PCR Kit - Protocol (100 rxns)

(TM41310) E. coli O157:H7 TaqMan Probe/Primer and Control Set - Protocol (100 rxns)

Safety Data Sheets:

(TM41350) E.coli O157:H7 TaqMan PCR Kit - Safety Data Sheet

Product Overview
Documentation

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E.coli O157:H7 TaqMan PCR Detection Kits

E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.

Click to expand options

E.coli O157:H7 TaqMan PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Supporting Data

TaqMan PCR Kit -Figure 1. Example of TaqMan PCR Positive result. Both PCR signals (FAM and Cy5) above the baseline from FAM, Cy5 and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 2. Example of TaqMan PCR Positive (Cy5) result. Both PCR signals above the baseline from Cy5 and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 3. Example of TaqMan PCR Positive (FAM) result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 4. Example of TaqMan PCR Negative result. No target DNA was detected in FAM and Cy5 channel but amplification signal from HEX indicates the successful PCR.

Click for expanded view

Kit Specifications

You have selected: Cat. TM41350

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

You have selected: Cat. TM41310

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Protocols:

(TM41350) E.coli O157:H7 TaqMan PCR Kit - Protocol (100 rxns)

(TM41310) E. coli O157:H7 TaqMan Probe/Primer and Control Set - Protocol (100 rxns)

Safety Data Sheets:

(TM41350) E.coli O157:H7 TaqMan PCR Kit - Safety Data Sheet