|E.coli O157:H7 TaqMan PCR Kit||TM41300||24 rxns|
|E.coli O157:H7 TaqMan Probe/Primer and Control Set||TM41310||100 rxns|
|E.coli O157:H7 End-Point PCR Kit||EP41300||24 rxns|
|E.coli O157:H7 Primer and Control Set||EP41310||100 rxns|
E.coli O157:H7 Detection Kits
E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.
There are 2 kits available for the detection of E.coli O157:H7:
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
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