For the rapid removal of endotoxins from up to 1 mg of previously purified DNA

  • Reduce endotoxin levels to 0.1 EU/µg DNA or less
  • Effectively remove endotoxins in as little as 30 minutes
  • Remove endotoxins from up to 1 mg of DNA
  • Rapid spin-column format

ItemsCat. #Size
Endotoxin Removal Kit (Maxi) - For DNA 21900 4 preps

Endotoxin Removal Kit (Maxi) - For DNA

This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. This kit reduces endotoxins to 0.1 EU/mg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. The convenient spin column procedure can be completed in approximately 30 minutes and typical recovery of DNA is >90% of the starting sample.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Kit Specifications
Maximum DNA Input
1 mg
Final Endotoxin Level
≤ 0.1 EU/µg DNA
Size of DNA Purified
Up to 13,000 bp
Maximum DNA Volume Input
3 mL
Average Recovery
> 90%
Time to Complete 4 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 1 year in their unopened containers.

Title Listeria-derived Listeriolysin O has PAMP-like properties independent of its hemolytic ability.
Journal Clinical and Vaccine Immunology. 2012.
Authors Wallecha A, Wood L, Pan ZK, Maciag PC, Shahabi V, Paterson Y.