Endotoxin Removal Kits- For DNA
For the rapid removal of endotoxins from previously purified DNA
![Endotoxin Removal Kit (Maxi) - For DNA](/sites/default/files/styles/norproduct_imagekit/public/images/product_images/21900-Reflection-web.jpg?itok=O7AF8jKB)
For research use only and NOT intended for in vitro diagnostics.
Endotoxin Removal Kits- For DNA
For the rapid removal of endotoxins from previously purified DNA
Overview
- Reduce endotoxin levels to 0.1 EU/µg DNA or less
- Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
- Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
- Fast and easy processing using a rapid spin-column format
These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
Details
Supporting Data
Figure 1. Efficiently Remove Endotoxins. An input of 11 µg of a plasmid in a 50 µL volume with an endotoxin level of over 8 EU/µg was subjected to clean-up by Norgen's Endotoxin Removal Kit. One microliter of the input and the 50 µL eluted DNA was diluted 1000-fold or 10 fold, respectively for a 100 µL end-point Limulus Amebocyte Lysate test for endotoxin level. The endotoxin levels of the plasmid DNA sample were reduced from over 8 EU/µg to less than or equal to 0.1 EU/µg of plasmid DNA using Norgen's Endotoxin Removal Kit (Mini), with high DNA recovery (see Figure 2).
Figure 2. High DNA Recovery. An input of 11 µg of a plasmid in a 50 µL volume with an endotoxin level of over 8 EU/µg was subjected to clean-up by Norgen's Endotoxin Removal Kit in triplicate. The 50 µL DNA elution was quantified by spectrophotometry. There is very little loss of DNA associated with using Norgen's Endotoxin Removal Kit (Mini). Recoveries of plasmid DNA are greater than 90% of the input amount when using this kit with efficient endotoxin removal (see Figure 1.)
Kit Specifications
|
|
Maximum DNA Input
|
25 μg
|
Final Endotoxin Level
|
≤ 0.1 EU/µg DNA
|
Size of DNA Purified
|
Up to 13,000 bp
|
Maximum DNA Volume Input |
100 μL
|
Average Recovery
|
> 90%
|
Time to Complete 10 Purifications |
20 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 22700 (25 preps) | Cat. 52200 (10 preps) | Cat. 21900 (4 preps) |
---|---|---|---|
Buffer SK | 15 mL | 60 mL | 60 mL |
Wash Solution H | 18 mL | 18 mL | 18 mL |
Elution Buffer I | 6 mL | 12 mL | 12 mL |
Endotoxin Removal Solution | 1.5 mL | 1.5 mL | 1.5 mL |
Precipitation Solution | - | 1.5 mL | 1.5 mL |
Spin Columns (assembled with Collection Tubes) | - | 10 | 4 |
Spin Columns | 25 | - | - |
Collection Tubes | 25 | - | - |
Elution Tubes (1.7 mL) | 25 | - | - |
Elution Tubes (15 mL) | - | 10 | - |
Elution Tubes (50 mL) | - | - | 4 |
Product Insert | 1 | 1 | 1 |
Documentation
Citations
Mini (22700)
Title | Highly modular bow-tie gene circuits with programmable dynamic behavior |
Journal | Nature Communications. 2016. |
Authors | Laura Prochazka, Bartolomeo Angelici, Benjamin Haefliger & Yaakov Benenson |
Title | Precision multidimensional assay for high-throughput microRNA drug discovery |
Journal | Nature Communications. 2016. |
Authors | Haefliger B, Prochazka L, Angelici B, Benenson Y |
Title | Transplantation of prokaryotic two-component signaling pathways into mammalian cells. |
Journal | Proceedings of the National Academy of Sciences. 2014. |
Authors | Jonathan Hansen, Erik Mailand, Krishna Kumar Swaminathan, Joerg Schreiber, Bartolomeo Angelici, and Yaakov Benenson. |
Title | Wheat amylase trypsin inhibitors drive intestinal inflammation via activation of toll-like receptor 4. |
Journal | The Journal of Experimental Medicine. 2012. |
Authors | Junker Y, Zeissig S, Kim SJ, Barisani D, Wieser H, Leffler DA, Zevallos V, Libermann TA, Dillon S, Freitag TL, Kelly CP, Schuppan D. |
Title | The costimulatory immunogen LPS induces the B-Cell clones that infiltrate transplanted human kidneys. |
Journal | Proceedings of the National Academy of Sciences of the United States of America. 2012. |
Authors | Grover RK, Cheng J, Peng Y, Jones TM, Ruiz DI, Ulevitch RJ, Glass JI, Dennis EA, Salomon DR, Lerner RA. |
Maxi (21900)
Title | Listeria-derived Listeriolysin O has PAMP-like properties independent of its hemolytic ability. |
Journal | Clinical and Vaccine Immunology. 2012. |
Authors | Wallecha A, Wood L, Pan ZK, Maciag PC, Shahabi V, Paterson Y. |