Endotoxin Removal Kits- For DNA For the rapid removal of endotoxins from previously purified DNA For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Endotoxin Removal Kits- For DNA For the rapid removal of endotoxins from previously purified DNA Price USD$275.00 SKU 22700 Format Mini Midi Maxi Size 25 Preps Quantity Overview Details Documentation Citations Overview Reduce endotoxin levels to 0.1 EU/µg DNA or less Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume Remove endotoxins from up to 1 mg of DNA with the Maxi format kit Fast and easy processing using a rapid spin-column format These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample. Endotoxin Removal Kit (Mini) This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes. Endotoxin Removal Kit (Midi) This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. Endotoxin Removal Kit (Maxi) This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. About Endotoxins Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications. Details Supporting Data Previous Figure 1. Efficiently Remove Endotoxins. An input of 11 µg of a plasmid in a 50 µL volume with an endotoxin level of over 8 EU/µg was subjected to clean-up by Norgen's Endotoxin Removal Kit. One microliter of the input and the 50 µL eluted DNA was diluted 1000-fold or 10 fold, respectively for a 100 µL end-point Limulus Amebocyte Lysate test for endotoxin level. The endotoxin levels of the plasmid DNA sample were reduced from over 8 EU/µg to less than or equal to 0.1 EU/µg of plasmid DNA using Norgen's Endotoxin Removal Kit (Mini), with high DNA recovery (see Figure 2). Figure 2. High DNA Recovery. An input of 11 µg of a plasmid in a 50 µL volume with an endotoxin level of over 8 EU/µg was subjected to clean-up by Norgen's Endotoxin Removal Kit in triplicate. The 50 µL DNA elution was quantified by spectrophotometry. There is very little loss of DNA associated with using Norgen's Endotoxin Removal Kit (Mini). Recoveries of plasmid DNA are greater than 90% of the input amount when using this kit with efficient endotoxin removal (see Figure 1.) Next Click for expanded view Kit Specifications Maximum DNA Input 25 μg Final Endotoxin Level ≤ 0.1 EU/µg DNA Size of DNA Purified Up to 13,000 bp Maximum DNA Volume Input 100 μL Average Recovery > 90% Time to Complete 10 Purifications 20 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 22700 (25 preps) Cat. 52200 (10 preps) Cat. 21900 (4 preps) Buffer SK 15 mL 60 mL 60 mL Wash Solution H 18 mL 18 mL 18 mL Elution Buffer I 6 mL 12 mL 12 mL Endotoxin Removal Solution 1.5 mL 1.5 mL 1.5 mL Precipitation Solution - 1.5 mL 1.5 mL Spin Columns (assembled with Collection Tubes) - 10 4 Spin Columns 25 - - Collection Tubes 25 - - Elution Tubes (1.7 mL) 25 - - Elution Tubes (15 mL) - 10 - Elution Tubes (50 mL) - - 4 Product Insert 1 1 1 Documentation PROTOCOLS (22700) Endotoxin Removal Kit (Mini) - Protocol (25 prep) (52200) Endotoxin Removal Kit (Midi) - Protocol (10 prep) (21900) Endotoxin Removal Kit (Maxi) - Protocol (4 preps) SAFETY DATA SHEETS (22700, 52200, 21900) Endotoxin Removal Kit - Safety Data Sheet FLYERS Flyer Citations Mini (22700) Title Highly modular bow-tie gene circuits with programmable dynamic behavior Journal Nature Communications. 2016. Authors Laura Prochazka, Bartolomeo Angelici, Benjamin Haefliger & Yaakov Benenson Title Precision multidimensional assay for high-throughput microRNA drug discovery Journal Nature Communications. 2016. Authors Haefliger B, Prochazka L, Angelici B, Benenson Y Title Transplantation of prokaryotic two-component signaling pathways into mammalian cells. Journal Proceedings of the National Academy of Sciences. 2014. Authors Jonathan Hansen, Erik Mailand, Krishna Kumar Swaminathan, Joerg Schreiber, Bartolomeo Angelici, and Yaakov Benenson. Title Wheat amylase trypsin inhibitors drive intestinal inflammation via activation of toll-like receptor 4. Journal The Journal of Experimental Medicine. 2012. Authors Junker Y, Zeissig S, Kim SJ, Barisani D, Wieser H, Leffler DA, Zevallos V, Libermann TA, Dillon S, Freitag TL, Kelly CP, Schuppan D. Title The costimulatory immunogen LPS induces the B-Cell clones that infiltrate transplanted human kidneys. Journal Proceedings of the National Academy of Sciences of the United States of America. 2012. Authors Grover RK, Cheng J, Peng Y, Jones TM, Ruiz DI, Ulevitch RJ, Glass JI, Dennis EA, Salomon DR, Lerner RA. Maxi (21900) Title Listeria-derived Listeriolysin O has PAMP-like properties independent of its hemolytic ability. Journal Clinical and Vaccine Immunology. 2012. Authors Wallecha A, Wood L, Pan ZK, Maciag PC, Shahabi V, Paterson Y. Related Products Genomic DNA Isolation Kit Fungi/Yeast Genomic DNA Isolation Kit Phage DNA Isolation Kit
Endotoxin Removal Kits- For DNA For the rapid removal of endotoxins from previously purified DNA For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Endotoxin Removal Kits- For DNA For the rapid removal of endotoxins from previously purified DNA Price USD$275.00 SKU 22700 Format Mini Midi Maxi Size 25 Preps Quantity
