Overview

• Allows for efficient labeling of exsosomes with very low levels of background
• Allows for labeling of exosomes isolated using various methods, including Norgen Biotek Corp. proprietary kits, ultracentrifugation and precipitation reagents
• Convenient & fast protocol, which includes both labeling and cleaning procedures
• Excitation at 590 nm/Emission at 617 nm

Norgen’s Exosomes Labeling and Cleaning Kit offers an all-in-one solution for the fluorescent labeling and clean-up of exosomes. This kit allows for specific labeling of exosomes using a fluorescent molecule, followed by their clean-up to minimize fluorescence background in further analysis.  The kit contains sufficient reagents to allow for 25 labelling and cleaning reactions.

Details

Supporting Data

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Figure 1. While the stain is selective to the exosomes lipidic membrane, the absence of cellular staining in presence of only the labeling molecule shows that the cleaning step is effective and leads to no labeling molecule carryover.

Figure 2. Intact exosomes were stained and cleaned without any labeling carryover

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Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature in the dark. This kit is stable for 2 years after the date of shipment.

Component	Cat. 68400 (25 rxns)
Reaction Buffer	30 mL
Labeling Soluton	36 μL
Slurry E	12.5 mL
ExoC Buffer	1.5 mL
ExoR Buffer	12 mL
Mini Filter Spin Columns	25
Elution Tubes (1.7 mL)	25
Product Insert	1

Documentation

PROTOCOLS

(68400) Exosome Labeling and Cleaning Kit - Protocol (25 Rxns)

SAFETY DATA SHEETS

68400 - Exosome Labeling and Cleaning Kit - SDS

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