Plasma/Serum Exosome Purification Kit All-in-one system for the purification of exosomes from different plasma/serum sample volumes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum Exosome Purification Kit All-in-one system for the purification of exosomes from different plasma/serum sample volumes Price USD$789.00 SKU 57400 Format Mini Midi Maxi Size 50 Preps Quantity Overview Details Documentation Citations Overview Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes. Versatile sample input ranging from 50 µL to 10 mL Plasma/Serum Exosome Purification Mini Kit (50 µL - 1 mL Plasma/Serum) Plasma/Serum Exosome Purification Midi Kit (1 mL - 4 mL Plasma/Serum) Plasma/Serum Exosome Purification Maxi Kit (4 mL - 10 mL Plasma/Serum) Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins. No precipitation reagents, overnight incubation, protease or coagulant treatments required No time-consuming ultracentrifugation, filtration or special syringes required Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type Purified exosomes are compatible with functional studies. Purified exosomes are free from any protein-bound cell-free circulating RNA Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration. Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method. The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression. NanoSight® Analysis Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration Exosmal RNA Analysis Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Plasma/Serum Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery. Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. Details Supporting Data Previous Figure 1. Intact exosomes were purified from 1 mL plasma using different purification methods. Intact exosomes were purified from 1 mL plasma using Norgen's Plasma/Serum Exosome Purification Mini Kit (Cat# 57400), Competitor S's kit and Competitor L's kit (from plasma). Exosomes purified using Norgen's kit were resuspended in 200 µL of Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit isolated 55 nm exosomes with a recovery of 9.64 x 1013 particles/mL plasma. No impurities were found to be contaminating the exosomes purified using Norgen's Plasma/Serum Exosome Purification Mini Kit. Additionally, exosomes with a broader size range covering from 50 nm - 150 nm were purified from 1 mL plasma with a higher concentration compared to the other two methods. Figure 2. Intact exosomes purified from 1 mL and 10 mL plasma. Intact exosomes were purified from 1 mL plasma using Norgen's Plasma/Serum Exosome Purification Mini Kit (Cat. 57400) and from 10 mL plasma using Norgen's Plasma/Serum Exosome Purification Maxi Kit (Cat. 57600). Exosomes purified using Norgen's Mini kit were resuspended in 200 µL of Norgen's ExoR buffer whereas exosomes purified using Norgen's Maxi kit were resuspended in 600 µL Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that the purification of exosomes is linear as 4.04 x 1010 particles/mL was recovered from 1 mL plasma whereas 2.95 x 1011 particles/mL was recovered from 10 mL plasma. Figure 3. TEM images of small EVs obtained from the same non disease frozen platelet free plasma (ND-frozen PFP) sample. Sample isolated with three commercial kits; representative of >10 images (scale bar, 200 nm). Title Human myeloma cell- and plasma-derived extracellular vesicles contribute to functional regulation of stromal cells. Journal Proteomics. 2021. Reale, A., Carmichael, I., Xu, R., Mithraprabhu, S., Khong, T., Chen, M., Fang, H., Savvidou, I., Ramachandran, M., Bingham, N. and Simpson, R.J. Figure 4. Western blot analysis of plasma small EVs (psEV). Plasma small EVs (psEV) isolated with the Norgen Biotek kit (#57400) using EV markers (TSG101, CD81, ALIX, CD63) and platelet markers (ITGA2B and SELP). Lysates of psEV derived from frozen Non-disease platelet free plasma (ND-PFP) are compared to lysates of psEV derived from fresh platelet free plasma (PFP). Cell free RNA BCT-Streck (sRNA collection tubes) and EDTA tubes are also compared (n= 3). Title Human myeloma cell- and plasma-derived extracellular vesicles contribute to functional regulation of stromal cells. Journal Proteomics. 2021. Reale, A., Carmichael, I., Xu, R., Mithraprabhu, S., Khong, T., Chen, M., Fang, H., Savvidou, I., Ramachandran, M., Bingham, N. and Simpson, R.J. Next Click for expanded view Kit Specifications Plasma/Serum Input (Cat. 57400) 50 μL - 1 mL Plasma/Serum Input (Cat. 57500) 1 mL - 4 mL Plasma/Serum Input (Cat. 57600) 4 mL - 10 mL Size of Exosomes Purified 40 nm - 150 nm Elution Volume Variable depending on the plasma/serum input volume Time to Complete 10 Purifications 15 - 30 minutes Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR. Component Cat. 57400 (50 preps) Cat. 57500 (25 preps) Cat. 57600 (15 preps) Slurry E 12.5 mL 12.5 mL 12.5 mL ExoC Buffer 8 mL 8 mL 2 x 8 mL ExoR Buffer 12 mL 12 mL 12 mL Mini Filter Spin Columns inserted into 2 mL tubes 50 25 15 Product Insert 1 1 1 Documentation PROTOCOLS (57400) Plasma/Serum Exosome Purification Kits - Protocol (50 prep) (57500) Plasma/Serum Exosome Purification Kits - Protocol (25 prep) (57600) Plasma/Serum Exosome Purification Kits - Protocol (15 prep) SAFETY DATA SHEETS (57400, 57500, 57600) Plasma/Serum Exosome Purification Kits - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Title Alteration of miRNAs in Small Neuron‑Derived Extracellular Vesicles of Alzheimer's Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses Journal Journal of Molecular Neuroscience . 2022. Authors Devrim Yagmur Durur, Bora Tastan, Kemal Ugur Tufekci, Melis Olcum, Hamdiye Uzuner, Gökhan Karakülah, Gorsev Yener, Sermin Genc Title Proteome profiling of neuron-derived exosomes in Alzheimer’s disease reveals hemoglobin as a potential biomarker Journal Neuroscience Letters. 2021. Authors Arioz, Burak Ibrahim, Kemal Ugur Tufekci, Melis Olcum, Devrim Yagmur Durur, Busra A. Akarlar, Nurhan Ozlu, H. Alper Bagriyanik, Pembe Keskinoglu, Görsev Yener, and Sermin Genc Title Human myeloma cell- and plasma-derived extracellular vesicles contribute to functional regulation of stromal cells Journal Proteomics. 2021. Authors Reale, A., Carmichael, I., Xu, R., Mithraprabhu, S., Khong, T., Chen, M., Fang, H., Savvidou, I., Ramachandran, M., Bingham, N. and Simpson, R.J. Related Services NGS RNA Isolation Related Products Urine Exosome Purification Mini Kit Exosomal RNA Isolation Kit
Plasma/Serum Exosome Purification Kit All-in-one system for the purification of exosomes from different plasma/serum sample volumes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum Exosome Purification Kit All-in-one system for the purification of exosomes from different plasma/serum sample volumes Price USD$789.00 SKU 57400 Format Mini Midi Maxi Size 50 Preps Quantity
