EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits

Overview

Purification and enrichment of intact cell culture media exosomes for functional studies
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

Figure 1. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Mini Kit. Calculations were relative to raw reads.

Figure 2. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Mini Kit.

Figure 3. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Mini Kit.

Figure 4. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit. Calculations were relative to raw reads.

Figure 5. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit.

Figure 6. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit.

Figure 7. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit. Calculations were relative to raw reads.

Figure 8. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit.

Figure 9. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various cell culture media volumes using EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit.

Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	5 ml to 10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.