EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Kits

Overview

Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact plasma/serum exosomes for functional studies.
Versatile plasma/serum input volume.
No phenol extractions, Proteinase K treatment, nor carrier RNA required.
No time-consuming ultracentrifugation, filtration nor special syringes required.
No precipitation reagents, nor overnight incubation required.
Compatible with plasma/serum from any species.
Pure exosomes are purified and are free-from any other RNA-binding proteins.
Purification is based on EXTRAClean Mini Spin Column chromatography that uses Norgen’s proprietary resin separation matrix.
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.

Norgen’s EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different plasma/serum sample volumes ranging from 50 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

Figure 1. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit. Calculations were relative to raw reads.

Figure 2. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit.

Figure 3. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit.

Figure 4. Average read quality distribution relative to raw reads of small RNA sequencing of exosomal RNA extracted from plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Midi Kit. Calculations were relative to raw reads.

Figure 5. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Midi Kit.

Figure 6. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Midi Kit.

Figure 7. Genome mapping distribution relative to input reads obtained from small RNA sequencing of exosomal RNA extracted from various plasma and serum samples using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit.

Figure 8. Average small RNA biotype distribution relative to the input reads obtained from sequencing of exosomal RNA extracted from various plasma and serum using EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit.

Kit Specifications
Sample Type	Exosomes purified using Norgen's Purification Kits
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Documentation

EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Kits