Features and Benefits

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Eight discrete fragments from 50 bp to 2000 bp
• Higher intensity reference band at 500 bp

The FastRunner DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains eight discrete fragments ranging from 50 bp to 2000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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Figure 1.  FastRunner DNA Ladder

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FastRunner DNA Ladder (Cat# 12800) - 100 loads

Ladder Properties:
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference

 

 

Fragment	Size (bp)	Mass (ng)
1	2000	104
2	1500	88
3	1000	68
4	750	59
5	500	93
6	300	28
7	150	35
8	50	25

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

 

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

PROTOCOLS

12800 - Product Insert

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders

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Detection of Genomic DNA from Seeds

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Determining the Molecular Weight Cut-Off of Plasma Circulating DNA Using Two Commercially Available Kits

SAFETY DATA SHEETS

12800 - FastRunner DNA Ladder - SDS

Citations

Title	Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events.
Journal	Epigenomics. 2012.
Authors	Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y.

Title	Development of a reliable dual-gene amplification RT-PCR assay for the detection of Turkey Meningoencephalitis virus in turkey brain tissues.
Journal	Journal of Virological Methods. 2012.
Authors	Irit D, Israel R, Amira AT, Khinich Y, Simanov M, Yuval C, Perk S, Lublin A.