Ideal for quick sizing of PCRs and restriction digests.

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Eight discrete fragments from 50 bp to 2000 bp
  • Higher intensity reference band at 500 bp

ItemsCat. #Size
FastRunner DNA Ladder 12800 100 loads

FastRunner DNA Ladder

The FastRunner DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains eight discrete fragments ranging from 50 bp to 2000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FastRunner DNA Ladder (Cat# 12800) - 100 loads

Ladder Properties:
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference
 

 

Fragment
Size (bp)
Mass (ng)
1
2000
104
2
1500
88
3
1000
68
4
750
59
5
500
93
6
300
28
7
150
35
8
50
25
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
 
Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
 
 

Title Development of a reliable dual-gene amplification RT-PCR assay for the detection of Turkey Meningoencephalitis virus in turkey brain tissues.
Journal Journal of Virological Methods. 2012.
Authors Irit D, Israel R, Amira AT, Khinich Y, Simanov M, Yuval C, Perk S, Lublin A.