FastRunner DNA Ladder
Ideal for quick sizing of PCRs and restriction digests.
For research use only and NOT intended for in vitro diagnostics.
FastRunner DNA Ladder
Ideal for quick sizing of PCRs and restriction digests.
$75.00
SKU
12800
Register today to receive an exclusive 15% off* on your first order.
Features and Benefits
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Eight discrete fragments from 50 bp to 2000 bp
- Higher intensity reference band at 500 bp
The FastRunner DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains eight discrete fragments ranging from 50 bp to 2000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
FastRunner DNA Ladder (Cat# 12800) - 100 loads
Ladder Properties:
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
2000
|
104
|
|
2
|
1500
|
88
|
|
3
|
1000
|
68
|
|
4
|
750
|
59
|
|
5
|
500
|
93
|
|
6
|
300
|
28
|
|
7
|
150
|
35
|
|
8
|
50
|
25
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
| Title | Development of a reliable dual-gene amplification RT-PCR assay for the detection of Turkey Meningoencephalitis virus in turkey brain tissues. |
| Journal | Journal of Virological Methods. 2012. |
| Authors | Irit D, Israel R, Amira AT, Khinich Y, Simanov M, Yuval C, Perk S, Lublin A. |