Fecal DNA Collection & Preservation Mini Tubes (Cat. 27650)

Fecal DNA Collection & Preservation Mini Tubes

Cat. 27650

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Fecal DNA Collection & Preservation Mini Tubes

Cat. 27650

A rapid and simple method for the preservation of DNA from fresh fecal samples

No need to immediately process samples
Includes pre-loaded beads for effective sample homogenization by bead beating (tubes are fully compatible with commercially available bead beating systems)
DNA preservation at room temperature over 2 years
Ship fecal samples at room temperature safely
Compatible with most DNA isolation methods

For research use only and NOT intended for in vitro diagnostics.

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Fecal DNA Collection & Preservation Mini Tubes
(Cat. 27650) 12 tubes

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Product Size

Kit Specifications

You have selected: Cat. 27650

Kit Specifications
Fecal Input	Maximum 30 mg (approximately 3-5 mouse droppings)
Stability of Fecal DNA at Room Temperature	2 years

Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Supporting Data

Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods..

Figure 2. Collection of Mouse Fecal Samples in Norgen's Fecal DNA Collection and Preservation Mini Tubes. Approximately 30 mg (3-5 droppings) of mouse fecal samples were added to Norgens Fecal Collection and Preservation Mini Tubes and briefly vortexed. The preserved samples are ready to ship, or for isolation of DNA using compatible DNA isolation kits.

Figure 3. Resolution of DNA Isolated from 200 µL Preserved Mouse Fecal Samples using Norgens Stool DNA Isolation Kit (Cat.27600). Briefly, 4-5 drops (<30 mg) of mouse fecal samples were collected using Norgen's Fecal DNA Collection and Preservation Mini Tubes (Cat. 27650). Stool DNA was isolated from the preserved samples using Norgen's Stool DNA Isolation Kit. For evaluation, 10 µL of each 50 µL elution were run on 1X TAE 1.2% agarose gel. Marker = Norgens HighRanger DNA Ladder.

Figure 4. Quality of Mouse Fecal DNA was Evaluated by 16s rDNAAmplification using Real-Time PCR. Two mouse fecal samples (red and blue) were collected and preserved using Norgens Fecal DNA Collection and Preservation Mini Tubes, and then 3 µL of DNA was used in 20 µL PCR reaction to detect 16s rDNA. No PCR inhibition was observed, indicating the high quality of DNA from mouse fecal samples for use indownstream applications.

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Component	Cat. 27650
Fecal DNA Collection & Preservation Mini Tubes (containing liquid preservative and beads)	12
Product Insert	1

Protocols:

(27650) Fecal DNA Collection & Preservation Mini Tubes - Protocol

Application Notes:

Comparative Microbiome Profiles from Fecal Preservation Methods

Gut Microbiome Diversity: Comparison of Stool DNA Preservation Methods

Safety Data Sheets:

(27650) Fecal DNA Collection & Preservation Mini Tubes - Safety Data Sheet

Posters:

Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives

Product Flyers:

Fecal DNA Collection & Preservation Tube

Product Overview
Documentation

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Fecal DNA Collection & Preservation Mini Tubes

Norgen’s Fecal DNA Collection & Preservation Mini Tubes are designed for the rapid preservation of DNA from fresh fecal specimens. These tubes contain Norgen’s Fecal DNA Preservative in a liquid format. Moreover, each collection tube is preloaded with beads for effective sample homogenization in any commercially available bead beater equipment (e.g. MP Biomedicals’ FastPrep®-24). The user simply collects fecal samples into the tubes and mixes by shaking or vortexing until the feces is mixed well with preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow the DNA in the samples to be preserved at room temperature for over 2 years.

This kit is highly suited to collection and preservation of fecal material from mouse, hamster, chinchilla, rabbit and more for microbiome studies and other applications.

DNA Isolation from Preservative

Fecal samples collected and preserved in Norgen’s Fecal DNA Collection and Preservation Mini Tubes are compatible with most fecal DNA isolation methods. Samples stored in the tubes have been used successfully with all of Norgen Biotek’s stool-based DNA isolation kits (please refer to Related Products table) and reagents.

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