Overview

Norgen’s Fecal DNA Collection & Preservation Mini Tubes are designed for the rapid preservation of DNA from fresh fecal specimens. These tubes contain Norgen’s Fecal DNA Preservative in a liquid format. Moreover, each collection tube is preloaded with beads for effective sample homogenization in any commercially available bead beater equipment (e.g. MP Biomedicals’ FastPrep®-24). The user simply collects fecal samples into the tubes and mixes by shaking or vortexing until the feces is mixed well with preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow the DNA in the samples to be preserved at room temperature for over 2 years. 

This kit is highly suited to collection and preservation of fecal material from mouse, hamster, chinchilla, rabbit and more for microbiome studies and other applications.

DNA Isolation from Preservative

Fecal samples collected and preserved in Norgen’s Fecal DNA Collection and Preservation Mini Tubes are compatible with most fecal DNA isolation methods. Samples stored in the tubes have been used successfully with all of Norgen Biotek’s stool-based DNA isolation kits (please refer to Related Products table) and reagents.  

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Supporting Data

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Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods..

Figure 2. Collection of Mouse Fecal Samples in Norgen's Fecal DNA Collection and Preservation Mini Tubes. Approximately 30 mg (3-5 droppings) of mouse fecal samples were added to Norgen’s Fecal Collection and Preservation Mini Tubes and briefly vortexed. The preserved samples are ready to ship, or for isolation of DNA using compatible DNA isolation kits. 

Figure 3. Resolution of DNA Isolated from 200 µL Preserved Mouse Fecal Samples using Norgen’s Stool DNA Isolation Kit (Cat. 27600). Briefly, 4-5 drops (<30 mg) of mouse fecal samples were collected using Norgen's Fecal DNA Collection and Preservation Mini Tubes (Cat. 27650). Stool DNA was isolated from the preserved samples using Norgen's Stool DNA Isolation Kit. For evaluation, 10 µL of each 50 µL elution were run on 1X TAE 1.2% agarose gel. Marker = Norgen’s HighRanger DNA Ladder. 

Figure 4. Quality of Mouse Fecal DNA was Evaluated by 16s rDNA Amplification using Real-Time PCR. Two mouse fecal samples (red and blue) were collected and preserved using Norgen’s Fecal DNA Collection and Preservation Mini Tubes, and then 3 µL of DNA was used in 20 µL PCR reaction to detect 16s rDNA. No PCR inhibition was observed, indicating the high quality of DNA from mouse fecal samples for use in downstream applications.

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