FFPE DNA Purification Kit Dx (Cat. Dx47400)
FFPE DNA Purification Kit Dx
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FFPE DNA Purification Kit Dx
For the rapid and efficient extraction and purification of DNA from FFPE samples for in vitro diagnostic use
- Fast and easy processing using rapid and convenient spin-columns
- Isolate high quality and high yield DNA
- DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping
Note: This product is not available for sale in the United States
Research version available here
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(Cat. Dx47400) 50 Preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity (gDNA)
|
10 μg
|
| Maximum Loading Volume Per Spin Column |
650 μL
|
| Size of DNA Purified |
All sizes > 80 bp
|
| Maximum Amount of Starting Material |
5 sections < 20 µm thick paraffin slices
25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K and RNase should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 2 years in their unopened containers.
Supporting Data
| Component | Cat. Dx47400 (50 preps) |
|---|---|
| Digestion Buffer | 20 mL |
| Binding Solution | 20 mL |
| Wash Solution | 22 mL |
| Elution Buffer | 12 mL |
| Proteinase K | 12 mg |
| RNase | 1 tube |
| gDNA Purification Micro Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin |
| Journal | Pakistan Journal of Biological Sciences. 2016. |
| Authors | T.M. Zohoncon, T.C. Ouedraogo, L.V.C. Brun, D. Obiri-Yeboah, W.F. Djigma, S. Kabibou, S. Ouattara, M. Gomina, A.T. Yonli, V.J.T.E. Bazie, C. Ouedraogo, O. Lompo, S.A. Akpona and J. Simpore |
FFPE DNA Purification Kit Dx
Norgen’s FFPE DNA Purification Kit Dx provides a rapid method for the isolation and purification of genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples for subsequent in vitro diagnostic use. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the DNA over time. Norgen’s FFPE DNA Purification Kit Dx provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of DNA. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The DNA is preferentially purified from other cellular components without the use of phenol or chloroform.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification. Any diagnostic results generated using the DNA isolated with Norgen’s FFPE DNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s FFPE DNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.
Norgen’s FFPE DNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity (gDNA)
|
10 μg
|
| Maximum Loading Volume Per Spin Column |
650 μL
|
| Size of DNA Purified |
All sizes > 80 bp
|
| Maximum Amount of Starting Material |
5 sections < 20 µm thick paraffin slices
25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K and RNase should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. Dx47400 (50 preps) |
|---|---|
| Digestion Buffer | 20 mL |
| Binding Solution | 20 mL |
| Wash Solution | 22 mL |
| Elution Buffer | 12 mL |
| Proteinase K | 12 mg |
| RNase | 1 tube |
| gDNA Purification Micro Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin |
| Journal | Pakistan Journal of Biological Sciences. 2016. |
| Authors | T.M. Zohoncon, T.C. Ouedraogo, L.V.C. Brun, D. Obiri-Yeboah, W.F. Djigma, S. Kabibou, S. Ouattara, M. Gomina, A.T. Yonli, V.J.T.E. Bazie, C. Ouedraogo, O. Lompo, S.A. Akpona and J. Simpore |
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