Olive Oil DNA Isolation Kit For the rapid preparation of genomic DNA from olive oil For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Olive Oil DNA Isolation Kit For the rapid preparation of genomic DNA from olive oil Price USD$179.00 SKU 61700 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Fast and easy processing using a rapid spin-column format Isolate total DNA without compromising total yield No phenol or chloroform extractions Isolate high quality total DNA from a variety of oil samples in order to support genetic traceability by allowing for the verification of the authenticity of the oil, thereby discouraging adulteration Norgen’s Olive Oil DNA Isolation Kit is designed for the rapid preparation of genomic DNA from olive oil in order to support genetic traceability by allowing for the verification of the authenticity of the oil, thereby discouraging adulteration. Norgen’s Olive Oil DNA Isolation Kit can also be used to purify DNA from other processed oils such as canola oil, sunflower oil or vegetable oil to allow for downstream analysis and testing for oil adulteration. Genomic DNA is efficiently extracted from 0.5 mL of oil by a combination of heat treatment, homogenization and a unique lysis buffer. PCR inhibitors are effectively removed during the washing steps and the purified genomic DNA is ready to use for downstream applications such as PCR and microarray analysis. Preparation time for ten samples is less then 30 minutes, and each kit contains sufficient materials for 50 preparations. Details Supporting Data Previous Figure 1. Process a Variety of Different Food Oils. A variety of different food oils purchased from stores can be processed for DNA isolation using Norgen's Olive Oil DNA Isolation Kit. The high quality of the purified DNA is shown in the Random Amplified Polymorphic DNA (RAPD) analysis (See Figure 2). Figure 2. Random Amplified Polymorphic DNA (RAPD) Analysis of 10 Food Oil Products. Inputs of 0.5 mL of oil were processed using Norgen’s Olive Oil DNA Isolation Kit, and 5 µL of the 40 µL eluted DNA was used in 20 µL of RAPD reaction. The RAPD pattern indicates successful RAPD amplification with high DNA quality. Ma: Norgen’s UltraRanger 1 kb DNA Ladder (Cat. 12100), Mb: HighRanger 1 kb DNA Ladder (Cat. 11900). Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 10 µg Maximum Column Loading Volumes 650 µL Time to Complete 10 Purifications 25 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. ComponentCat. 61700 (50 preps)Lysis Buffer L60 mLBinding Buffer B40 mLWash Solution A38 mLElution Buffer B8 mLHomogenization Tubes50Spin Columns50Collection Tubes50Elution Tubes (1.7 mL)50Product Insert1 Documentation PROTOCOLS (61700) Olive Oil DNA Isolation Kit - Protocol (50 preps) SAFETY DATA SHEETS (61700) Olive Oil DNA Isolation Kit - Safety Data Sheet Citations Title Application of high resolution melting combined with DNA-based markers for quantitative analysis of olive oil authenticity and adulteration Journal Food Chemistry. 2020 Authors Elsa Chedid, Myrto Rizou, Panagiotis Kalaitzis Related Products Water RNA/DNA Purification Kit - 0.22 µm Fungi/Yeast Genomic DNA Isolation Kit Plant/Fungi DNA Isolation Kit
Olive Oil DNA Isolation Kit For the rapid preparation of genomic DNA from olive oil For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Olive Oil DNA Isolation Kit For the rapid preparation of genomic DNA from olive oil Price USD$179.00 SKU 61700 Format Spin Column Size 50 Preps Quantity
