Fungi/Yeast Genomic DNA Isolation Kits

Figure 2. DNA can be Isolated from Spores and Mycelium. Genomic DNA was extracted from the spores and mycelium of Botrytis cinera using Norgen's Fungi/Yeast Genomic DNA Isolation Kit. For the spores, 10⁵ spores/mL were collected from a plate culture by adding 3 mL of 0.9% NaCl to the fungi culture plate and gently shaking to extract the spores. For mycelium, 20 mg of mycellium was collected with sterilized forceps. DNA was isolated using Norgen's Fungi/Yeast Genomic DNA Isolation Kit with the provided Bead Tubes. For analysis, 10 µL from each 100 µL elution was loaded on a 1% 1xTAE agarose gel. Lane M: Norgen's HighRanger 1kb DNA Ladder. Optional RNase treatment was not performed during the extraction. As it can be seen, DNA could be successfully isolated from both the spores and mycelium.

Figure 3. Identification of Fungal and Yeast Species in a Real-time PCR (SYBR Green). DNA was isolated from 50 mg samples (wet weight) of yeast (Pichia sp) and fungi including Aspergillus niger, Cladosporium cladasporioides, Botrytis cinerea and Mucor racemosus using Norgen's Fungi/Yeast Genomic DNA Isolation Kit, and 2 µL of each of the 100 µL DNA elutions was used in a qPCR (SYBR Green) reaction with specific fungal and yeast primers. The qPCR was successful in amplifying and detecting all the yeast and fungal DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.

Figure 4. High Yields of DNA Isolated from Saccharomyces cerevisiae overnight culture. DNA was isolated from S. cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit. Following isolation of 10 samples, 10 µL from each 100 µL elution was loaded on 1% TAE agarose gel. Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit demonstrated a good and consistent DNA yield and integrity. Lane M: Norgen's HighRanger 1kb DNA Ladder.

Figure 5. High quality of yeast DNA measured by NanoDrop. DNA isolated from Saccharomyces cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit showed a high quality of DNA based on A260/280 and A260/230. This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications.

Figure 6. Purified DNA Can be Amplified in a Real-time PCR (SYBER GREEN) Reaction. DNA was isolated from Saccharomyces cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit. Five µL of the DNA from each 100 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with S. cerevisiae primers. The real-time PCR was successful in amplifying the S. cerevisiae from all 10 isolated samples. This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.