Fungi/Yeast Genomic DNA Isolation Kits For the rapid purification of DNA from yeast cells and fungal spores or mycelium For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Fungi/Yeast Genomic DNA Isolation Kits For the rapid purification of DNA from yeast cells and fungal spores or mycelium Price USD$179.00 SKU 27300 Format Spin Column High Throughput Size 50 Preps Quantity Overview Details Documentation Citations Overview Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positiveBead tubes (provided) allow for effective mechanical homogenizationPurified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more. Fungi/Yeast Genomic DNA Isolation Kit (Spin Column) This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations. Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT) Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes. Details Supporting Data Previous Figure 1. DNA Isolation from Different Fungi Species and Yeast. To demonstrate the purification of DNA from different fungal species, 30 mg of fungi were collected from plate cultures of Pichia sp, Aspergillus niger, Cladosporium cladasporioides, Botrytis cinerea and Mucor racemosus, and the DNA was extracted using Norgen's Fungi/Yeast Genomic DNA Isolation Kit. The bead system efficiently lysed the fungal cell walls with the provided Lysis Solution, and total DNA was eluted in 100 µL. For analysis, 10 µL from each elution was loaded in 1% 1xTAE agarose gel. Lane 1: Yeast (Pichia sp.), Lane 2: Aspergillus Niger; Lane 3: Cladosporium cladosporioides; Lane 4: Botrytis cinerea; Lane 5: Mucor racemosus; Lane M: Norgen's HighRanger 1kb DNA Ladder. The optional RNase treatment was not performed during the process. Figure 2. DNA can be Isolated from Spores and Mycelium. Genomic DNA was extracted from the spores and mycelium of Botrytis cinera using Norgen's Fungi/Yeast Genomic DNA Isolation Kit. For the spores, 105 spores/mL were collected from a plate culture by adding 3 mL of 0.9% NaCl to the fungi culture plate and gently shaking to extract the spores. For mycelium, 20 mg of mycellium was collected with sterilized forceps. DNA was isolated using Norgen's Fungi/Yeast Genomic DNA Isolation Kit with the provided Bead Tubes. For analysis, 10 µL from each 100 µL elution was loaded on a 1% 1xTAE agarose gel. Lane M: Norgen's HighRanger 1kb DNA Ladder. Optional RNase treatment was not performed during the extraction. As it can be seen, DNA could be successfully isolated from both the spores and mycelium. Figure 3. Identification of Fungal and Yeast Species in a Real-time PCR (SYBR Green). DNA was isolated from 50 mg samples (wet weight) of yeast (Pichia sp) and fungi including Aspergillus niger, Cladosporium cladasporioides, Botrytis cinerea and Mucor racemosus using Norgen's Fungi/Yeast Genomic DNA Isolation Kit, and 2 µL of each of the 100 µL DNA elutions was used in a qPCR (SYBR Green) reaction with specific fungal and yeast primers. The qPCR was successful in amplifying and detecting all the yeast and fungal DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Figure 4. High Yields of DNA Isolated from Saccharomyces cerevisiae overnight culture. DNA was isolated from S. cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit. Following isolation of 10 samples, 10 µL from each 100 µL elution was loaded on 1% TAE agarose gel. Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit demonstrated a good and consistent DNA yield and integrity. Lane M: Norgen's HighRanger 1kb DNA Ladder. Figure 5. High quality of yeast DNA measured by NanoDrop. DNA isolated from Saccharomyces cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit showed a high quality of DNA based on A260/280 and A260/230. This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. Figure 6. Purified DNA Can be Amplified in a Real-time PCR (SYBER GREEN) Reaction. DNA was isolated from Saccharomyces cerevisiae overnight culture using Norgen's Fungi/Yeast Genomic DNA Isolation 96-Well Kit. Five µL of the DNA from each 100 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with S. cerevisiae primers. The real-time PCR was successful in amplifying the S. cerevisiae from all 10 isolated samples. This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control. Next Click for expanded view Kit Specifications Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Maximum Amount of Starting Material: Fungi (wet weight) Yeast or Bacteria Culture 50 mg 0.5mL - 1mL Average Yield* Pichia sp. (yeast) Botrytis cinerea Fusarium sp. Aspergillis niger Mucor racemosus Cladosporium cladosporioides Penicillium sp. Rhizopus oryzae Alternaria tenuissima 25 μg 32 μg 42 μg 26 μg 15 μg 12 μg 40 μg 7 μg 30 μg Time to Complete 10 purifications 30 minutes * Yield will vary depending on the type of sample processed Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 27300 (50 preps) Cat. 27350 (192 preps) Lysis Buffer L 30 mL 2 x 60 mL Resuspension Solution A 20 mL 60 mL Solution BX 28 mL 2 x 28 mL Wash Solution A 18 mL 2 x 38 mL Elution Buffer B 15 mL 30 mL Bead Tubes 50 200 Spin Columns 50 - Collection Tubes 50 - 96-Well Plate - 2 96-Well Collection Plate - 2 Adhesive Tape - 4 Elution Tubes (1.7 mL) 50 - 96-Well Elution Plate - 2 Product Insert 1 1 Documentation PROTOCOLS (27300) Fungi/Yeast Genomic DNA Isolation Kit (Spin Column) - Protocol (50 prep) (27350) Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT) - Protocol (2 x 96-well) APPLICATION NOTES Detection of Genomic DNA from Seeds Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods SAFETY DATA SHEETS 27300, 27350 - Safety Data Sheet Citations Spin Column (27300) Title Biocontrol of root knot nematodes by endophytic fungus isolated from garlic Journal Scientia Horticulturae. 2024. Authors Aimal Khan, Anwar Hussain, Farman Ali, Muhammad Hamayun, Amjad Iqbal, Hosam O. Elansary, Ho-youn Kim Title Sources of genomic diversity in the self-fertile plant pathogen, Sclerotinia sclerotiorum, and consequences for resistance breeding Journal PLOS ONE. 2022. Authors Lone Buchwaldt, Harsh Garg, Krishna D. Puri, Jonathan Durkin, Jennifer Adam, Myrtle Harrington, Debora Liabeuf, Alan Davies, Dwayne D. Hegedus, Andrew G. Sharpe, Krishna Kishore Gali Title Molecular identification of Aspergillus fumigatus complex from lung transplant recipients using multilocus sequencing analysis (MLSA) Journal Official Journal of the Association of Medical Microbiology and Infectious Disease Canada. 2022. Authors Ghulam Dhabaan, MSc, PhD, Julianne Kus, HBSc, MSc, PhD, FCCM, Deepali Kumar, MD, FRCPC, Atul Humar, MD, FRCPC, Shahid Husain, MD, MS, FECMM, FRCP(Edin), Tony Mazzulli, MD, FRCPC, FACP Title Quinone Outside Inhibitor Resistance Conferred with the G143A Substitution in Corynespora cassiicola, Which Causes Target Spot, from Arkansas and Mississippi Soybean Journal APS Publications. 2022. Authors Xiaopeng Wang, N. Braham D. S. Dhillon, Nicole Brochard, Nina Aboughanem-Sabanadzovic, Sead Sabanadzovic, Maria Tomaso-Peterson, Tessie H. Wilkerson, Burt H. Bluhm, Travis R. Faske, and Tom W. Allen Title Isolation and Identification of Candida dubliniensis in Patient with CervicalVulvovaginal Candidiasis in Erbil City Journal Rafidain Journal of Science. 2022. Authors Nareen Q. Faqe Abdulla and Hero M. Ismael Title Occurrence of Spot Blotch in Spring Barley Caused by Bipolaris sorokiniana Shoem. in South-Eastern Kazakhstan Journal The Scientific World Journal. 2022. Authors Yerlan Dutbayev, Nurlan Kuldybayev, Saule Daugaliyeva, Elvira Ismailova, Nadira Sultanova, Göksel Özer, Ayana Slyamova, Kadyrzhan Mukin, Abdelfattah Dababat, and Minura Yessimbekova Title Evaluation of three DNA extraction methods from fungal cultures Journal Med J Armed Forces India. 2017. Authors Mahadevan Kumar, Col, and M. Mugunthan Title Thermomyces lanuginosus STm: A source of thermostable hydrolytic enzymes for novel application in extraction of high-quality natural rubber from Taraxacum kok-saghyz (Rubber dandelion) Journal Industrial Crops and Products. 2017. Authors Shomaila Sikandar, Victor C. Ujor, Thaddeus C. Ezeji, Jesse L. Rossington, Frederick C. Michel Jr., Colleen M. McMahan, Naeem Ali, Katrina Cornish Title The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks Journal Microbial Cell Factories. 2016. Authors Xiong L,, Kameshwar AK, Chen X, Guo Z, Mao C, Chen S, Qin W. Title Premature failure of utility poles in Switzerland and Germany related to wood decay basidiomycetes Journal International Journal of the Biology, Chemistry, Physics, and Technology of Wood. 2016. Authors Javier Ribera, Mark Schubert, Siegfried Fink, Marco Cartabia, Francis W.M.R. Schwarze Title Phylogeographic Analysis of Blastomyces dermatitidis and Blastomyces gilchristii Reveals an Association with North American Freshwater Drainage Basins Journal PLoS One. 2016. Authors McTaggart, L. R., Brown, E. M., & Richardson, S. E Title Natural occurrence of Alternaria toxins in pomegranate fruit and the influence of some technological processing on their levels in juice Journal Acta Alimentaria. 2016. Authors H.M. Elhariry, G.M. Khiralla, Y. Gherbawy, H. Abd ElRahman Title Isolation and Characterization of Hydrocarbon Utilizing Yeast (HUY) Isolates from Palm Wine Journal American Journal of Molecular Biology. 2016. Authors Okerentugba, P. O., Ataikiru, T. L., & Ichor, T Title Barcode Sequencing Screen Identifies SUB1 as a Regulator of Yeast Pheromone Inducible Genes Journal G3: Genes, Genomes, Genetics. 2016. Authors Sliva, A., Kuang, Z., Meluh, P. B., & Boeke, J. D Title Engineered Production of Tryprostatins in E. coli through Reconstitution of a Partial ftm Biosynthetic Gene Cluster from Aspergillus sp. Journal Jacbobs Journal of Biotechnology and Bioengineering. 2015. Authors Shah GR, Wesener SR, Cheng YQ Title An endophytic fungus isolated from finger millet (Eleusine coracana) produces anti-fungal natural productsi Journal Frontiers in Microbiology. 2015. Authors Walaa K. Mousa, Adrian Schwan, Jeffrey Davidson, Philip Strange, Huaizhi Liu, Ting Zhou, France-Isabelle Auzanneau and Manish N. Raizada Title Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi Journal International Journal of Extensive Research. 2015. Authors Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey Title Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil Journal Microbiology Insights. 2015. Authors Tournas VH, Niazi NS, Kohn JS Title Fungal Presence in Selected Tree Nuts and Dried Fruits Journal Microbiology Insights. 2015. Authors Tournas VH, Niazi NS, Kohn JS Title Occurrence of QoI fungicide resistance in Cercospora sojina from Mississippi soybean Journal Plant Disease. 2015. Authors J. R. Standish, M. Tomaso-Peterson, T. W. Allen, S. Sabanadzovic, N. Aboughanem-Sabanadzovic Title Structure and Absolute Configuration of 20β-Hydroxyprednisolone, a Biotransformed Product of Predinisolone by the Marine Endophytic Fungus Penicilium lapidosum. Journal Molecules. 2014. Authors Sadia Sultan, Muhammad Zaimi bin Mohd Noor, El Hassane Anouar, Syed Adnan Ali Shah, Fatimah Salim, Rohani Rahim, Zuhra Bashir Khalifa Al Trabolsy, and Jean-Frédéric Faizal Weber. Title Amplification of TRP1 for specific detection of Phytophthora ramorum. Journal United States Patent Application 2014 022057. 2014. Authors Robert C. Bohannon, Paul F. Russell, JR. Title Effective method for the heat inactivation of Blastomyces dermatitidis. Journal Medical Mycology. 2014. Authors Elizabeth M. Brown, Lisa R. McTaggart, Donald E. Low and Susan E. Richardson. Title Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei. Journal BioMed Research International. 2014. Authors Yuanyuan Hong, Mehdi Dashtban, Greg Kepka, Sanfeng Chen, and Wensheng Qin. Title "Innovation And Enhancement Of Almond Processing Chain", A Project To Highlight The Almond Dried Fruit Quality In Sicily (South Italy). Journal International Society for Horticultural Science. 2014. Authors C. Oliveri, D. Scalone, G. Muratore, G. Spagna , R. La Rosa. Title Sea foam as a source of fungal inoculum for the isolation of biologically active natural products. Journal Mycology: An International Journal on Fungal Biology. 2012. Authors David P. Overy, Fabrice Berrue, Hebelin Correa, Novriyandi Hanif, Kathryn Hay, Martin Lanteigne, Kathrine Mquilian, Stephanie Duffy, Patricia Boland, Ramesh Jagannathan, Gavin S. Carr, Marieke Vansteeland & Russell G. Kerr. Title Increased expression of β-glucosidase A in Clostridium thermocellum 27405 significantly increases cellulase activity. Journal Bioengineered. 2012. Authors Maki ML, Armstrong L, Leung KT, Qin W. Title Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers. Journal International Journal of Biochemistry and Molecular Biology. 2011. Authors Maki M, Broere M, Leung K, Qin W. HT (27350) Title Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases Journal Cell Reports. 2016. Authors Tamari Z, Barkai N Related Products Bacterial Genomic DNA Isolation Kit Plant RNA/DNA Purification Kit Plant/Fungi DNA Isolation Kit
Fungi/Yeast Genomic DNA Isolation Kits For the rapid purification of DNA from yeast cells and fungal spores or mycelium For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Fungi/Yeast Genomic DNA Isolation Kits For the rapid purification of DNA from yeast cells and fungal spores or mycelium Price USD$179.00 SKU 27300 Format Spin Column High Throughput Size 50 Preps Quantity