For the rapid purification of DNA from yeast cells and fungal spores or mycelium

  • Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive
  • Bead tubes (provided) allow for effective mechanical homogenization
  • Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more

ItemsCat. #Size
Fungi/Yeast Genomic DNA Isolation Kit 27300 50 preps

Fungi/Yeast Genomic DNA Isolation Kit

This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Protocol time is typically less then 30 minutes. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.

Kit Specifications
Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes
Maximum Amount of Starting Material:
Fungi (wet weight)
Yeast or Bacteria Culture
50 mg
1 x 108cfu's
Average Purity (OD260/280)
(~0.5 - 1 mL)
Average Yield*
Pichia sp. (yeast)
Botrytis cinerea
​Fusarium sp.
Aspergillis niger
Penicillium sp.
Alternaria tenuissima
25 μg
32 μg
42 μg
26 μg
40 μg
30 μg

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 27300 (50 preps)
Lysis Buffer L 30 mL
Resuspension Solution A 20 mL
Solution BX 28 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Occurrence of QoI fungicide resistance in Cercospora sojina from Mississippi soybean
Journal Plant Disease. 2015.
Authors J. R. Standish, M. Tomaso-Peterson, T. W. Allen, S. Sabanadzovic, N. Aboughanem-Sabanadzovic
Title Structure and Absolute Configuration of 20β-Hydroxyprednisolone, a Biotransformed Product of Predinisolone by the Marine Endophytic Fungus Penicilium lapidosum.
Journal Molecules. 2014.
Authors Sadia Sultan, Muhammad Zaimi bin Mohd Noor, El Hassane Anouar, Syed Adnan Ali Shah, Fatimah Salim, Rohani Rahim, Zuhra Bashir Khalifa Al Trabolsy, and Jean-Frédéric Faizal Weber.
Title Amplification of TRP1 for specific detection of Phytophthora ramorum.
Journal United States Patent Application 2014 022057. 2014.
Authors Robert C. Bohannon, Paul F. Russell, JR.
Title Effective method for the heat inactivation of Blastomyces dermatitidis.
Journal Medical Mycology. 2014.
Authors Elizabeth M. Brown, Lisa R. McTaggart, Donald E. Low and Susan E. Richardson.
Title Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei.
Journal BioMed Research International. 2014.
Authors Yuanyuan Hong, Mehdi Dashtban, Greg Kepka, Sanfeng Chen, and Wensheng Qin.
Title "Innovation And Enhancement Of Almond Processing Chain", A Project To Highlight The Almond Dried Fruit Quality In Sicily (South Italy).
Journal International Society for Horticultural Science. 2014.
Authors C. Oliveri, D. Scalone, G. Muratore, G. Spagna , R. La Rosa.
Title Amplification of TRP1 for specific detection of Phytophthora ramorum.
Journal US Grant Application. 2014.
Authors Robert C. Bohannon, Paul F. Russell, JR.
Title Sea foam as a source of fungal inoculum for the isolation of biologically active natural products.
Journal Mycology: An International Journal on Fungal Biology. 2012.
Authors David P. Overy, Fabrice Berrue, Hebelin Correa, Novriyandi Hanif, Kathryn Hay, Martin Lanteigne, Kathrine Mquilian, Stephanie Duffy, Patricia Boland, Ramesh Jagannathan, Gavin S. Carr, Marieke Vansteeland & Russell G. Kerr.
Title Increased expression of β-glucosidase A in Clostridium thermocellum 27405 significantly increases cellulase activity.
Journal Bioengineered. 2012.
Authors Maki ML, Armstrong L, Leung KT, Qin W.
Title Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers.
Journal International Journal of Biochemistry and Molecular Biology. 2011.
Authors Maki M, Broere M, Leung K, Qin W.