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For the rapid purification of DNA from yeast cells and fungal spores or mycelium
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Column Binding Capacity |
50 μg
|
Maximum Column Loading Volume |
650 μL
|
Maximum Amount of Starting Material: Fungi (wet weight) Yeast or Bacteria Culture |
50 mg 0.5mL - 1mL |
Average Yield* Pichia sp. (yeast) Botrytis cinerea Fusarium sp. Aspergillis niger Mucor racemosus Cladosporium cladosporioides Penicillium sp. Rhizopus oryzae Alternaria tenuissima |
25 μg 32 μg 42 μg 26 μg 15 μg 12 μg 40 μg 7 μg 30 μg |
Time to Complete 10 purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Binding Capacity Per Well
|
50 μg
|
Maximum Loading Volume Per Well
|
500 μL
|
Size of DNA Purified
|
All sizes |
Maximum Amount of Starting Material: Fungi (Wet weight) Yeast or Gram-positive bacterial culture |
50 mg 0.5 mL - 1 mL |
Time to Complete 96 Purifications
|
40 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.
Supporting Data
Spin Column (27300)
Title | Sources of genomic diversity in the self-fertile plant pathogen, Sclerotinia sclerotiorum, and consequences for resistance breeding |
Journal | PLOS ONE. 2022. |
Authors | Lone Buchwaldt, Harsh Garg, Krishna D. Puri, Jonathan Durkin, Jennifer Adam, Myrtle Harrington, Debora Liabeuf, Alan Davies, Dwayne D. Hegedus, Andrew G. Sharpe, Krishna Kishore Gali |
Title | Molecular identification of Aspergillus fumigatus complex from lung transplant recipients using multilocus sequencing analysis (MLSA) |
Journal | Official Journal of the Association of Medical Microbiology and Infectious Disease Canada. 2022. |
Authors | Ghulam Dhabaan, MSc, PhD, Julianne Kus, HBSc, MSc, PhD, FCCM, Deepali Kumar, MD, FRCPC, Atul Humar, MD, FRCPC, Shahid Husain, MD, MS, FECMM, FRCP(Edin), Tony Mazzulli, MD, FRCPC, FACP |
Title | Quinone Outside Inhibitor Resistance Conferred with the G143A Substitution in Corynespora cassiicola, Which Causes Target Spot, from Arkansas and Mississippi Soybean |
Journal | APS Publications. 2022. |
Authors | Xiaopeng Wang, N. Braham D. S. Dhillon, Nicole Brochard, Nina Aboughanem-Sabanadzovic, Sead Sabanadzovic, Maria Tomaso-Peterson, Tessie H. Wilkerson, Burt H. Bluhm, Travis R. Faske, and Tom W. Allen |
Title | Isolation and Identification of Candida dubliniensis in Patient with CervicalVulvovaginal Candidiasis in Erbil City |
Journal | Rafidain Journal of Science. 2022. |
Authors | Nareen Q. Faqe Abdulla and Hero M. Ismael |
Title | Occurrence of Spot Blotch in Spring Barley Caused by Bipolaris sorokiniana Shoem. in South-Eastern Kazakhstan |
Journal | The Scientific World Journal. 2022. |
Authors | Yerlan Dutbayev, Nurlan Kuldybayev, Saule Daugaliyeva, Elvira Ismailova, Nadira Sultanova, Göksel Özer, Ayana Slyamova, Kadyrzhan Mukin, Abdelfattah Dababat, and Minura Yessimbekova |
Title | Evaluation of three DNA extraction methods from fungal cultures |
Journal | Med J Armed Forces India. 2017. |
Authors | Mahadevan Kumar, Col, and M. Mugunthan |
Title | Thermomyces lanuginosus STm: A source of thermostable hydrolytic enzymes for novel application in extraction of high-quality natural rubber from Taraxacum kok-saghyz (Rubber dandelion) |
Journal | Industrial Crops and Products. 2017. |
Authors | Shomaila Sikandar, Victor C. Ujor, Thaddeus C. Ezeji, Jesse L. Rossington, Frederick C. Michel Jr., Colleen M. McMahan, Naeem Ali, Katrina Cornish |
Title | The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks |
Journal | Microbial Cell Factories. 2016. |
Authors | Xiong L,, Kameshwar AK, Chen X, Guo Z, Mao C, Chen S, Qin W. |
Title | Premature failure of utility poles in Switzerland and Germany related to wood decay basidiomycetes |
Journal | International Journal of the Biology, Chemistry, Physics, and Technology of Wood. 2016. |
Authors | Javier Ribera, Mark Schubert, Siegfried Fink, Marco Cartabia, Francis W.M.R. Schwarze |
Title | Phylogeographic Analysis of Blastomyces dermatitidis and Blastomyces gilchristii Reveals an Association with North American Freshwater Drainage Basins |
Journal | PLoS One. 2016. |
Authors | McTaggart, L. R., Brown, E. M., & Richardson, S. E |
Title | Natural occurrence of Alternaria toxins in pomegranate fruit and the influence of some technological processing on their levels in juice |
Journal | Acta Alimentaria. 2016. |
Authors | H.M. Elhariry, G.M. Khiralla, Y. Gherbawy, H. Abd ElRahman |
Title | Isolation and Characterization of Hydrocarbon Utilizing Yeast (HUY) Isolates from Palm Wine |
Journal | American Journal of Molecular Biology. 2016. |
Authors | Okerentugba, P. O., Ataikiru, T. L., & Ichor, T |
Title | Barcode Sequencing Screen Identifies SUB1 as a Regulator of Yeast Pheromone Inducible Genes |
Journal | G3: Genes, Genomes, Genetics. 2016. |
Authors | Sliva, A., Kuang, Z., Meluh, P. B., & Boeke, J. D |
Title | Engineered Production of Tryprostatins in E. coli through Reconstitution of a Partial ftm Biosynthetic Gene Cluster from Aspergillus sp. |
Journal | Jacbobs Journal of Biotechnology and Bioengineering. 2015. |
Authors | Shah GR, Wesener SR, Cheng YQ |
Title | An endophytic fungus isolated from finger millet (Eleusine coracana) produces anti-fungal natural productsi |
Journal | Frontiers in Microbiology. 2015. |
Authors | Walaa K. Mousa, Adrian Schwan, Jeffrey Davidson, Philip Strange, Huaizhi Liu, Ting Zhou, France-Isabelle Auzanneau and Manish N. Raizada |
Title | Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi |
Journal | International Journal of Extensive Research. 2015. |
Authors | Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey |
Title | Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil |
Journal | Microbiology Insights. 2015. |
Authors | Tournas VH, Niazi NS, Kohn JS |
Title | Fungal Presence in Selected Tree Nuts and Dried Fruits |
Journal | Microbiology Insights. 2015. |
Authors | Tournas VH, Niazi NS, Kohn JS |
Title | Occurrence of QoI fungicide resistance in Cercospora sojina from Mississippi soybean |
Journal | Plant Disease. 2015. |
Authors | J. R. Standish, M. Tomaso-Peterson, T. W. Allen, S. Sabanadzovic, N. Aboughanem-Sabanadzovic |
Title | Structure and Absolute Configuration of 20β-Hydroxyprednisolone, a Biotransformed Product of Predinisolone by the Marine Endophytic Fungus Penicilium lapidosum. |
Journal | Molecules. 2014. |
Authors | Sadia Sultan, Muhammad Zaimi bin Mohd Noor, El Hassane Anouar, Syed Adnan Ali Shah, Fatimah Salim, Rohani Rahim, Zuhra Bashir Khalifa Al Trabolsy, and Jean-Frédéric Faizal Weber. |
Title | Amplification of TRP1 for specific detection of Phytophthora ramorum. |
Journal | United States Patent Application 2014 022057. 2014. |
Authors | Robert C. Bohannon, Paul F. Russell, JR. |
Title | Effective method for the heat inactivation of Blastomyces dermatitidis. |
Journal | Medical Mycology. 2014. |
Authors | Elizabeth M. Brown, Lisa R. McTaggart, Donald E. Low and Susan E. Richardson. |
Title | Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei. |
Journal | BioMed Research International. 2014. |
Authors | Yuanyuan Hong, Mehdi Dashtban, Greg Kepka, Sanfeng Chen, and Wensheng Qin. |
Title | "Innovation And Enhancement Of Almond Processing Chain", A Project To Highlight The Almond Dried Fruit Quality In Sicily (South Italy). |
Journal | International Society for Horticultural Science. 2014. |
Authors | C. Oliveri, D. Scalone, G. Muratore, G. Spagna , R. La Rosa. |
Title | Sea foam as a source of fungal inoculum for the isolation of biologically active natural products. |
Journal | Mycology: An International Journal on Fungal Biology. 2012. |
Authors | David P. Overy, Fabrice Berrue, Hebelin Correa, Novriyandi Hanif, Kathryn Hay, Martin Lanteigne, Kathrine Mquilian, Stephanie Duffy, Patricia Boland, Ramesh Jagannathan, Gavin S. Carr, Marieke Vansteeland & Russell G. Kerr. |
Title | Increased expression of β-glucosidase A in Clostridium thermocellum 27405 significantly increases cellulase activity. |
Journal | Bioengineered. 2012. |
Authors | Maki ML, Armstrong L, Leung KT, Qin W. |
Title | Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers. |
Journal | International Journal of Biochemistry and Molecular Biology. 2011. |
Authors | Maki M, Broere M, Leung K, Qin W. |
HT (27350)
Title | Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases |
Journal | Cell Reports. 2016. |
Authors | Tamari Z, Barkai N |
Fungi/Yeast Genomic DNA Isolation Kits
These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.
Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)
This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.
Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)
Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Column Binding Capacity |
50 μg
|
Maximum Column Loading Volume |
650 μL
|
Maximum Amount of Starting Material: Fungi (wet weight) Yeast or Bacteria Culture |
50 mg 0.5mL - 1mL |
Average Yield* Pichia sp. (yeast) Botrytis cinerea Fusarium sp. Aspergillis niger Mucor racemosus Cladosporium cladosporioides Penicillium sp. Rhizopus oryzae Alternaria tenuissima |
25 μg 32 μg 42 μg 26 μg 15 μg 12 μg 40 μg 7 μg 30 μg |
Time to Complete 10 purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Kit Specifications
|
|
Binding Capacity Per Well
|
50 μg
|
Maximum Loading Volume Per Well
|
500 μL
|
Size of DNA Purified
|
All sizes |
Maximum Amount of Starting Material: Fungi (Wet weight) Yeast or Gram-positive bacterial culture |
50 mg 0.5 mL - 1 mL |
Time to Complete 96 Purifications
|
40 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.
Spin Column (27300)
Title | Sources of genomic diversity in the self-fertile plant pathogen, Sclerotinia sclerotiorum, and consequences for resistance breeding |
Journal | PLOS ONE. 2022. |
Authors | Lone Buchwaldt, Harsh Garg, Krishna D. Puri, Jonathan Durkin, Jennifer Adam, Myrtle Harrington, Debora Liabeuf, Alan Davies, Dwayne D. Hegedus, Andrew G. Sharpe, Krishna Kishore Gali |
Title | Molecular identification of Aspergillus fumigatus complex from lung transplant recipients using multilocus sequencing analysis (MLSA) |
Journal | Official Journal of the Association of Medical Microbiology and Infectious Disease Canada. 2022. |
Authors | Ghulam Dhabaan, MSc, PhD, Julianne Kus, HBSc, MSc, PhD, FCCM, Deepali Kumar, MD, FRCPC, Atul Humar, MD, FRCPC, Shahid Husain, MD, MS, FECMM, FRCP(Edin), Tony Mazzulli, MD, FRCPC, FACP |
Title | Quinone Outside Inhibitor Resistance Conferred with the G143A Substitution in Corynespora cassiicola, Which Causes Target Spot, from Arkansas and Mississippi Soybean |
Journal | APS Publications. 2022. |
Authors | Xiaopeng Wang, N. Braham D. S. Dhillon, Nicole Brochard, Nina Aboughanem-Sabanadzovic, Sead Sabanadzovic, Maria Tomaso-Peterson, Tessie H. Wilkerson, Burt H. Bluhm, Travis R. Faske, and Tom W. Allen |
Title | Isolation and Identification of Candida dubliniensis in Patient with CervicalVulvovaginal Candidiasis in Erbil City |
Journal | Rafidain Journal of Science. 2022. |
Authors | Nareen Q. Faqe Abdulla and Hero M. Ismael |
Title | Occurrence of Spot Blotch in Spring Barley Caused by Bipolaris sorokiniana Shoem. in South-Eastern Kazakhstan |
Journal | The Scientific World Journal. 2022. |
Authors | Yerlan Dutbayev, Nurlan Kuldybayev, Saule Daugaliyeva, Elvira Ismailova, Nadira Sultanova, Göksel Özer, Ayana Slyamova, Kadyrzhan Mukin, Abdelfattah Dababat, and Minura Yessimbekova |
Title | Evaluation of three DNA extraction methods from fungal cultures |
Journal | Med J Armed Forces India. 2017. |
Authors | Mahadevan Kumar, Col, and M. Mugunthan |
Title | Thermomyces lanuginosus STm: A source of thermostable hydrolytic enzymes for novel application in extraction of high-quality natural rubber from Taraxacum kok-saghyz (Rubber dandelion) |
Journal | Industrial Crops and Products. 2017. |
Authors | Shomaila Sikandar, Victor C. Ujor, Thaddeus C. Ezeji, Jesse L. Rossington, Frederick C. Michel Jr., Colleen M. McMahan, Naeem Ali, Katrina Cornish |
Title | The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks |
Journal | Microbial Cell Factories. 2016. |
Authors | Xiong L,, Kameshwar AK, Chen X, Guo Z, Mao C, Chen S, Qin W. |
Title | Premature failure of utility poles in Switzerland and Germany related to wood decay basidiomycetes |
Journal | International Journal of the Biology, Chemistry, Physics, and Technology of Wood. 2016. |
Authors | Javier Ribera, Mark Schubert, Siegfried Fink, Marco Cartabia, Francis W.M.R. Schwarze |
Title | Phylogeographic Analysis of Blastomyces dermatitidis and Blastomyces gilchristii Reveals an Association with North American Freshwater Drainage Basins |
Journal | PLoS One. 2016. |
Authors | McTaggart, L. R., Brown, E. M., & Richardson, S. E |
Title | Natural occurrence of Alternaria toxins in pomegranate fruit and the influence of some technological processing on their levels in juice |
Journal | Acta Alimentaria. 2016. |
Authors | H.M. Elhariry, G.M. Khiralla, Y. Gherbawy, H. Abd ElRahman |
Title | Isolation and Characterization of Hydrocarbon Utilizing Yeast (HUY) Isolates from Palm Wine |
Journal | American Journal of Molecular Biology. 2016. |
Authors | Okerentugba, P. O., Ataikiru, T. L., & Ichor, T |
Title | Barcode Sequencing Screen Identifies SUB1 as a Regulator of Yeast Pheromone Inducible Genes |
Journal | G3: Genes, Genomes, Genetics. 2016. |
Authors | Sliva, A., Kuang, Z., Meluh, P. B., & Boeke, J. D |
Title | Engineered Production of Tryprostatins in E. coli through Reconstitution of a Partial ftm Biosynthetic Gene Cluster from Aspergillus sp. |
Journal | Jacbobs Journal of Biotechnology and Bioengineering. 2015. |
Authors | Shah GR, Wesener SR, Cheng YQ |
Title | An endophytic fungus isolated from finger millet (Eleusine coracana) produces anti-fungal natural productsi |
Journal | Frontiers in Microbiology. 2015. |
Authors | Walaa K. Mousa, Adrian Schwan, Jeffrey Davidson, Philip Strange, Huaizhi Liu, Ting Zhou, France-Isabelle Auzanneau and Manish N. Raizada |
Title | Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi |
Journal | International Journal of Extensive Research. 2015. |
Authors | Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey |
Title | Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil |
Journal | Microbiology Insights. 2015. |
Authors | Tournas VH, Niazi NS, Kohn JS |
Title | Fungal Presence in Selected Tree Nuts and Dried Fruits |
Journal | Microbiology Insights. 2015. |
Authors | Tournas VH, Niazi NS, Kohn JS |
Title | Occurrence of QoI fungicide resistance in Cercospora sojina from Mississippi soybean |
Journal | Plant Disease. 2015. |
Authors | J. R. Standish, M. Tomaso-Peterson, T. W. Allen, S. Sabanadzovic, N. Aboughanem-Sabanadzovic |
Title | Structure and Absolute Configuration of 20β-Hydroxyprednisolone, a Biotransformed Product of Predinisolone by the Marine Endophytic Fungus Penicilium lapidosum. |
Journal | Molecules. 2014. |
Authors | Sadia Sultan, Muhammad Zaimi bin Mohd Noor, El Hassane Anouar, Syed Adnan Ali Shah, Fatimah Salim, Rohani Rahim, Zuhra Bashir Khalifa Al Trabolsy, and Jean-Frédéric Faizal Weber. |
Title | Amplification of TRP1 for specific detection of Phytophthora ramorum. |
Journal | United States Patent Application 2014 022057. 2014. |
Authors | Robert C. Bohannon, Paul F. Russell, JR. |
Title | Effective method for the heat inactivation of Blastomyces dermatitidis. |
Journal | Medical Mycology. 2014. |
Authors | Elizabeth M. Brown, Lisa R. McTaggart, Donald E. Low and Susan E. Richardson. |
Title | Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei. |
Journal | BioMed Research International. 2014. |
Authors | Yuanyuan Hong, Mehdi Dashtban, Greg Kepka, Sanfeng Chen, and Wensheng Qin. |
Title | "Innovation And Enhancement Of Almond Processing Chain", A Project To Highlight The Almond Dried Fruit Quality In Sicily (South Italy). |
Journal | International Society for Horticultural Science. 2014. |
Authors | C. Oliveri, D. Scalone, G. Muratore, G. Spagna , R. La Rosa. |
Title | Sea foam as a source of fungal inoculum for the isolation of biologically active natural products. |
Journal | Mycology: An International Journal on Fungal Biology. 2012. |
Authors | David P. Overy, Fabrice Berrue, Hebelin Correa, Novriyandi Hanif, Kathryn Hay, Martin Lanteigne, Kathrine Mquilian, Stephanie Duffy, Patricia Boland, Ramesh Jagannathan, Gavin S. Carr, Marieke Vansteeland & Russell G. Kerr. |
Title | Increased expression of β-glucosidase A in Clostridium thermocellum 27405 significantly increases cellulase activity. |
Journal | Bioengineered. 2012. |
Authors | Maki ML, Armstrong L, Leung KT, Qin W. |
Title | Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers. |
Journal | International Journal of Biochemistry and Molecular Biology. 2011. |
Authors | Maki M, Broere M, Leung K, Qin W. |
HT (27350)
Title | Natural Diversity in Pentose Fermentation Is Explained by Variations in Histone Deacetylases |
Journal | Cell Reports. 2016. |
Authors | Tamari Z, Barkai N |