Genomic DNA Isolation Kit For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Genomic DNA Isolation Kit For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs Price USD$165.00 SKU 24700 Format Spin Column Size 50 Preps 100 Preps 250 Preps Quantity Overview Details Documentation Citations Overview Isolate genomic DNA from animal tissues, cells, bodily fluids, viruses and swabs Rapid and convenient spin column procedure Purified DNA is of the highest quality and integrity for sensitive downstream applications including PCR, qPCR, genotyping, sequencing and more This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time). Details Supporting Data Previous Figure 1. Isolation of High Quality Genomic DNA. Genomic DNA was isolated from various sample types using Norgen's Genomic DNA Isolation Kit. Lanes 1-3 contain genomic DNA that was isolated from four different samples containing 5 x 105 HeLa cells, while lanes 4 and 5 contain genomic DNA that was isolated from 5 mg of heart tissue. All the purified genomic is of the highest quality and integrity. Lane M is the Norgen HighRanger 1kb DNA Ladder. For all purified DNA, 10 µL of each 200 µL elution were resolved on a 1X TAE, 1% agarose DNA gel. Figure 2. Full Compatibility with Digests. Genomic DNA was isolated from 10 mg of kidney tissue using Norgen's Genomic DNA Isolation Kit, and subsequently digested. Twenty microliters of the 200 µL eluted DNA were used in a 50 µL restriction digestion with 10 units of either Hind III or Nde I (overnight at 37°C). Fifteen microliters of the digested DNA were then loaded on a 1X TAE, 1% agarose gel. Lane 1 contains undigested Norgen-isolated DNA, while Lanes 2 contain the genomic DNA digested with Hind III and Lanes 3 contain the genomic DNA digested with Nde I. Lane M is Norgen's HighRanger 1kb DNA Ladder. Next Click for expanded view Kit Specifications Column Binding Capacity 25 µg Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney) 8 µg 10 µg Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension 20 mg 3 x 106 cells 150 µL 150 µL Time to Complete 10 Purifications 80 minutes * Yield will vary depending on the type of sample processed Storage Conditions and Product Stability The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 24700 (50 preps) Cat. 24750 (100 preps) Cat. 24770 (250 preps) Digestion Buffer A 25 mL 2 x 25 mL 5 x 25 mL Buffer SK 30 mL 2 x 30 mL 5 x 30 mL Wash Solution A 18 mL 2 x 18 mL 5 x 18 mL Elution Buffer B 30 mL 2 x 30 mL 5 x 30 mL Proteinase K 12 mg 2 x 12 mg 5 x 12 mg Spin Columns 50 100 250 Collection Tubes 50 100 250 Elution Tubes (1.7 mL) 50 100 250 Product Insert 1 1 1 Documentation PROTOCOLS (24700) Genomic DNA Isolation Kit - Protocol (50 prep) (24750) Genomic DNA Isolation Kit - Protocol (100 prep) (24770) Genomic DNA Isolation Kit - Protocol (250 prep) SAFETY DATA SHEETS (24700, 24750) Genomic DNA Isolation Kit - Safety Data Sheet Citations Title Repurposing Itraconazole and Hydroxychloroquine to Target Lysosomal Homeostasis in Epithelial Ovarian Cancer Journal Cancer Research Communications. 2022 Authors Stefano Marastoni, Ainhoa Madariaga, Aleksandra Pesic, Sree Narayanan Nair, Zhu Juan Li, Zvi Shalev, Troy Ketela, et al. 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Genomic DNA Isolation Kit For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Genomic DNA Isolation Kit For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs Price USD$165.00 SKU 24700 Format Spin Column Size 50 Preps 100 Preps 250 Preps Quantity
