Giardia intestinalis TaqMan RT-PCR Detection Kits

Cat. TM43850, TM43810

Giardia intestinalis TaqMan RT-PCR Detection Kits

Cat.

For research use only and NOT intended for in vitro diagnostics.

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Giardia intestinalis TaqMan RT-PCR Kit
(Cat. TM43850) 100 rxns

Giardia intestinalis TaqMan Probe/Primer and Control Set
(Cat. TM43810) 100 rxns

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Format: RT-PCR
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:100 rxns

Overview

Giardia intestinalis TaqMan RT-PCR Detection Kits

Detection kits for Giardia intestinalis
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis.

Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.

There are 2 kits available for the detection of Giardia intestinalis:

Click to expand options

Giardia intestinalis TaqMan RT-PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

Giardia intestinalis TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit -Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqManPCR Kit-Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

Kit Specifications

Cat.

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

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Giardia intestinalis TaqMan RT-PCR Detection Kits (Cat. TM43850, TM43810)

Giardia intestinalis TaqMan RT-PCR Detection Kits

Giardia intestinalis TaqMan RT-PCR Detection Kits

Format: RT-PCR
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:100 rxns

Overview

Giardia intestinalis TaqMan RT-PCR Detection Kits

Details

Supporting Data

Kit Specifications

Documentation

Citations

Giardia intestinalis TaqMan RT-PCR Detection Kits (Cat. TM43850, TM43810)

Giardia intestinalis TaqMan RT-PCR Detection Kits

Giardia intestinalis TaqMan RT-PCR Detection Kits

Format: RT-PCR Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:100 rxns

Overview

Giardia intestinalis TaqMan RT-PCR Detection Kits

Details

Supporting Data

Kit Specifications

Documentation

Citations

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Format: RT-PCR
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.