Overview

Pneumocystis jirovecii is a yeast-like fungus that causes Pneumocystis jirovecii Pneumonia (originally known as Pneumocystis carinii Pneumonia or PCP). PCP is the most common opportunistic infection in patients with HIV/AIDS. Species of Pneumocystis are commonly found in the lungs of healthy individuals. In fact, most children are believed to be exposed by age 3 or 4 years. Studies have suggested that P. jirovecii is communicable, possibly via airborne transmission. Disease usually develops in patients whose cellular immunity and humoral immunity are defective. Even with the widespread use of highly active antiretroviral therapy (HAART), there is still a high prevalence of PCP in HIV patients.

For research use only and NOT intended for in vitro diagnostics.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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