HighRanger 1 kb DNA Ladder
Ideal for size determination of digested DNA
For research use only and NOT intended for in vitro diagnostics.
Powered by Bioz HighRanger 1 kb DNA Ladder
Ideal for size determination of digested DNA
$75.00
SKU
11900
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Overview
- Ready-to-use
- Quantitative
- Precise
- Highly Stable
- Thirteen discrete fragments ranging from 300 bp to 10000 bp
- Higher intensity reference band at 5000 bp
The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
HighRanger 1kb DNA Ladder (Cat# 11900) - 100 loads
Ladder Properties:
• Thirteen discrete bands, ranging from 300 bp to 10000 bp
• Higher intensity band at 5000 bp for easy reference
• Thirteen discrete bands, ranging from 300 bp to 10000 bp
• Higher intensity band at 5000 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
10000
|
58
|
|
2
|
8000
|
53
|
|
3
|
6000
|
46
|
|
4
|
5000
|
67
|
|
5
|
4000
|
56
|
|
6
|
3000
|
47
|
|
7
|
2500
|
41
|
|
8
|
2000
|
34
|
|
9
|
1500
|
29
|
|
10
|
1000
|
18
|
|
11
|
700
|
13
|
| 12 | 500 |
23 |
| 13 | 300 |
14 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | A new microsporidium species, Nucleospora braziliensis n. Sp. infecting Nile tilapia (Oreochromis niloticus) from brazilian aquaculture |
| Journal | International Journal of Fisheries and aquatic Studies. 2017. |
| Authors | Marianna Vaz Rodrigues, Claire Juliana Francisco, Gianmarco Silva David, Reinaldo José da Silva and João Pessoa Araújo Júnior |
| Title | Distribution Profile of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolates from Asa River (Nigeria) |
| Journal | Journal of Applied Sciences and Environmental Management. 2017. |
| Authors | Matthew Kolawole Olatunji, Albert Olayemi, Fausat Jimoh |
| Title | Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean |
| Journal | Brazilian Journal of Microbiology. 2016. |
| Authors | Frédéric Zakhi, Habib Jeder, Odile Domergue, Anne Willems, Jean-Claude Cleyet-Marel, Monique Gillis, Bernard Dreyfus, Philippe de Lajudie |
| Title | Sinuolinea niloticus n. sp., a myxozoan parasite that causes disease in Nile tilapia (Oreochromis niloticus) |
| Journal | Parasitology Research. 2015. |
| Authors | Rodrigues, M. V., Francisco, C. J., Biondi, G. F., & Júnior, J. P. A |
| Title | Resistance to Cowpea aphid-borne mosaic virus in species and hybrids of Passiflora: advances for the control of the passion fruit woodiness disease in Brazil |
| Journal | European Journal of Plant Pathology. 2015. |
| Authors | Eileen Azevedo Santos, Alexandre Pio Viana, Josie Cloviane de Oliveira Freitas, Fernando Higino de Lima e Silva, Rosana Rodrigues, Marcelo Eiras |
| Title | Unraveling a mechanism of honey antibacterial action: Polyphenol/H2O2-induced oxidative effect on bacterial cell growth and on DNA degradation. |
| Journal | Food Chemistry. 2012. |
| Authors | Brudzynski K, Abubaker K, Miotto D. |
| Title | Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. |
| Journal | Frontiers in Microbiology. 2011. |
| Authors | Brudzynski K, Abubaker K, Laurent M, Castle A. |