HighRanger 1 kb DNA Ladder
Ideal for size determination of digested DNA
For research use only and NOT intended for in vitro diagnostics.
HighRanger 1 kb DNA Ladder
Ideal for size determination of digested DNA
SKU
11900
Features and Benefits
- Ready-to-use
- Quantitative
- Precise
- Highly Stable
- Thirteen discrete fragments ranging from 300 bp to 10000 bp
- Higher intensity reference band at 5000 bp
The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
HighRanger 1kb DNA Ladder (Cat# 11900) - 100 loads
Ladder Properties:
• Thirteen discrete bands, ranging from 300 bp to 10000 bp
• Higher intensity band at 5000 bp for easy reference
• Thirteen discrete bands, ranging from 300 bp to 10000 bp
• Higher intensity band at 5000 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
10000
|
58
|
|
2
|
8000
|
53
|
|
3
|
6000
|
46
|
|
4
|
5000
|
67
|
|
5
|
4000
|
56
|
|
6
|
3000
|
47
|
|
7
|
2500
|
41
|
|
8
|
2000
|
34
|
|
9
|
1500
|
29
|
|
10
|
1000
|
18
|
|
11
|
700
|
13
|
| 12 | 500 |
23 |
| 13 | 300 |
14 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean |
| Citation | Brazilian Journal of Microbiology 2023. |
| Authors | Cardoso, A. A., de Paula Andraus, M., de Oliveira Borba, T. C., Martin-Didonet, C. C. G., & de Brito Ferreira, E. P |
| Title | Haplosporidium pinnae Parasite Detection in Seawater Samples |
| Citation | Microorganisms 2023. |
| Authors | Irene Moro-Martínez ,Maite Vázquez-Luis ,José Rafael García-March ,Patricia Prado ,Milena Micic and Gaetano Catanese |
| Title | Prevalence and characterization of Trypanosoma species from livestock of Cholistan desert of Pakistan |
| Citation | Tropical Biomedicine 2023. |
| Authors | Sobia, M., Mirza, I. S., Nuzhat, S., Sonia, T., Abul, H., Hafiz, M. A., ... & Muhammad, F. Q. (2018). |
| Title | Sinuolinea niloticus n. sp., a myxozoan parasite that causes disease in Nile tilapia (Oreochromis niloticus) |
| Citation | Parasitology Research 2023. |
| Authors | Rodrigues, M. V., Francisco, C. J., Biondi, G. F., & Júnior, J. P. A |