Ideal for size determination of digested DNA

  • Ready-to-use
  • Quantitative
  • Precise
  • Highly Stable
  • Thirteen discrete fragments ranging from 300bp to 10000bp
  • Higher intensity reference band at 5000bp

Items Cat. # Size
HighRanger 1kb DNA Ladder 11900 100 loads

HighRanger 1 kb DNA Ladder

The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

  • Ready-to-use
  • Quantitative
  • Precise
  • Highly Stable

HighRanger 1kb DNA Ladder (Cat# 11900) - 100 loads

Ladder Properties:
• Thirteen discrete bands, ranging from 300bp to 10,000bp.
• Higher intensity band at 5000bp for easy reference.

 

Fragment
Size (bp)
Mass (ng)
1
10000
58
2
8000
53
3
6000
46
4
5000
67
5
4000
56
6
3000
47
7
2500
41
8
2000
34
9
1500
29
10
1000
18
11
700
13
12 500

23

13 300

14

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
 
Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Title Resistance to Cowpea aphid-borne mosaic virus in species and hybrids of Passiflora: advances for the control of the passion fruit woodiness disease in Brazil
Journal European Journal of Plant Pathology. 2015.
Authors Eileen Azevedo Santos, Alexandre Pio Viana, Josie Cloviane de Oliveira Freitas, Fernando Higino de Lima e Silva, Rosana Rodrigues, Marcelo Eiras
Title Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey.
Journal Frontiers in Microbiology. 2011.
Authors Brudzynski K, Abubaker K, Laurent M, Castle A.