HighRanger Plus 100 bp DNA Ladder (Cat. 12000)
HighRanger Plus 100 bp DNA Ladder
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HighRanger Plus 100 bp DNA Ladder
Ideal for size determination of digested DNA
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Nineteen discrete fragments ranging from 100Â bp to 10000Â bp
- Higher intensity reference bands at 500Â bp, 1000Â bp and 5000Â bp
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 12000) 100 loads
Product Size
Kit Specifications
HighRanger Plus 100 bp DNA Ladder (Cat# 12000) - 100 loads
Ladder Properties:
- Nineteen discrete fragments ranging from 100 bp to 10000 bp
- Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
10000
|
42
|
|
2
|
8000
|
33
|
|
3
|
6000
|
25
|
|
4
|
5000
|
42
|
|
5
|
4000
|
35
|
|
6
|
3000
|
27
|
|
7
|
2500
|
22
|
|
8
|
2000
|
18
|
|
9
|
1500
|
13
|
|
10
|
1000
|
40
|
|
11
|
900
|
28
|
| 12 | 800 |
25 |
| 13 | 700 |
34 |
| 14 | 600 |
19 |
| 15 | 500 |
44 |
| 16 | 400 |
13 |
| 17 | 300 |
18 |
| 18 | 200 |
13 |
| 19 | 100 |
10 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Supporting Data
| Title | Phylogeny and recombination analysis of Brazilian yellow passion fruit isolates of Cowpea aphid-borne mosaic virus: origin and relationship with hosts. |
| Journal | Australasian Plant Pathology. 2014. |
| Authors | Leilane K. Rodrigues, Leonardo A. Silva, Renata M. Garcêz, Alexandre L. R. Chaves, Ligia M. L. Duarte, José S. Giampani, Addolorata Colariccio, Ricardo Harakava, Marcelo Eiras. |
| Title | Gender Identification by Calls and Body Size of the Streaked Shearwater Examined by CHD Genes. |
| Journal | BioOne. 2014. |
| Authors | Hiroshi Arima1, Nariko Oka, Yoshiyuki Baba, Hisashi Sugawa and Takahiro Ota. |
HighRanger Plus 100 bp DNA Ladder
The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100Â bp to 10000Â bp with higher intensity reference bands at 500Â bp, 1000Â bp and 5000Â bp. This Ladder is ideal for size determination of digested DNA.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Supporting Data
Kit Specifications
HighRanger Plus 100 bp DNA Ladder (Cat# 12000) - 100 loads
Ladder Properties:
- Nineteen discrete fragments ranging from 100 bp to 10000 bp
- Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
10000
|
42
|
|
2
|
8000
|
33
|
|
3
|
6000
|
25
|
|
4
|
5000
|
42
|
|
5
|
4000
|
35
|
|
6
|
3000
|
27
|
|
7
|
2500
|
22
|
|
8
|
2000
|
18
|
|
9
|
1500
|
13
|
|
10
|
1000
|
40
|
|
11
|
900
|
28
|
| 12 | 800 |
25 |
| 13 | 700 |
34 |
| 14 | 600 |
19 |
| 15 | 500 |
44 |
| 16 | 400 |
13 |
| 17 | 300 |
18 |
| 18 | 200 |
13 |
| 19 | 100 |
10 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
| Title | Phylogeny and recombination analysis of Brazilian yellow passion fruit isolates of Cowpea aphid-borne mosaic virus: origin and relationship with hosts. |
| Journal | Australasian Plant Pathology. 2014. |
| Authors | Leilane K. Rodrigues, Leonardo A. Silva, Renata M. Garcêz, Alexandre L. R. Chaves, Ligia M. L. Duarte, José S. Giampani, Addolorata Colariccio, Ricardo Harakava, Marcelo Eiras. |
| Title | Gender Identification by Calls and Body Size of the Streaked Shearwater Examined by CHD Genes. |
| Journal | BioOne. 2014. |
| Authors | Hiroshi Arima1, Nariko Oka, Yoshiyuki Baba, Hisashi Sugawa and Takahiro Ota. |