• Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Nineteen discrete fragments ranging from 100bp to 10000bp
  • Higher intensity reference bands at 500bp, 1000bp and 5000bp

Items Cat. # Size
HighRanger Plus 100bp DNA Ladder 12000 100 loads

HighRanger Plus 100 bp DNA Ladder

The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100bp to 10000bp with higher intensity reference bands at 500bp, 1000bp and 5000bp. This Ladder is ideal for size determination of digested DNA.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise

HighRanger Plus 100 bp DNA Ladder (Cat# 12000) - 100 loads

Ladder Properties:

  • Nineteen discrete fragments ranging from 100bp to 10000bp.
  • Higher intensity bands at 500bp, 1000bp and 5000bp for easy reference.

 

Fragment
Size (bp)
Mass (ng)
1
10000
42
2
8000
33
3
6000
25
4
5000
42
5
4000
35
6
3000
27
7
2500
22
8
2000
18
9
1500
13
10
1000
40
11
900
28
12 800

25

13 700

34

14 600

19

15 500

44

16 400

13

17 300

18

18 200

13

19 100

10

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Title Phylogeny and recombination analysis of Brazilian yellow passion fruit isolates of Cowpea aphid-borne mosaic virus: origin and relationship with hosts.
Journal Australasian Plant Pathology. 2014.
Authors Leilane K. Rodrigues, Leonardo A. Silva, Renata M. Garcêz, Alexandre L. R. Chaves, Ligia M. L. Duarte, José S. Giampani, Addolorata Colariccio, Ricardo Harakava, Marcelo Eiras.
Title Gender Identification by Calls and Body Size of the Streaked Shearwater Examined by CHD Genes.
Journal BioOne. 2014.
Authors Hiroshi Arima1, Nariko Oka, Yoshiyuki Baba, Hisashi Sugawa and Takahiro Ota.