Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) TaqMan RT-PCR Detection Kits Price USD$949.00 SKU TM69950 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview PCR-based kit for Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) detection Includes a PCR control to monitor PCR inhibition and validate sample quality Available in real-time TaqMan format Includes Primer & Probe Mix, positive and negative control to confirm the integrity of the reagents Pair with Norgen's Plant/Fungi RNA Purification Kit for the highest level of sensitivity in detection Multiplexed TaqMan PCR-based kit for Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) detection. Norgen's turn-key solution comes with Master Mix, Primer & Probe Mix, Positive control, and Nuclease free water. Norgen's TaqMan Master Mix includes an internal PCR control to monitor PCR inhibition and validate sample quality. Pair with Norgen's Plant/Fungi RNA Purification Kit for the highest level of sensitivity in detection. This kit is designed for research use only and not for use in diagnostic procedures. TaqMan PCR Kit Click to expand options HLVd/LCV/CanCV TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents HLVd/LCV/CanCV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous Figure 1. Example of TaqMan One-step RT-PCR Positive result. PCR signals above the baseline from FAM, Tex615, Cy5, and HEX channel indicate the successful PCR. Individual amplification in any of the channels also indicates a positive result for the pathogen corresponding to that channel. Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM,TEX615, and Cy5 channels but amplification signal from HEX indicates the successful PCR. Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from all targets was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Channel Selection for Specified Platforms HLVd LCV CanCV Internal Control Platform FAM Texas 615/ *Texas Red Cy5 HEX BioRad CFX96 Touch™ Real-Time PCR Detection System FAM JUN/*ROX Cy5 VIC QuantStudioTM Green Orange Red Yellow Qiagen Rotor-Gene Q Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM69950 (100 preps) Cat. TM69910 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - HLVd/LCV/CanCV Primer & Probe Mix 280 μL 280 μL HLVd/LCV/CanCV Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM69950) HLVd/LCV/CanCV TaqMan RT-PCR Kit - Protocol (100 rxns) APPLICATION NOTES Sensitivity and Specificity of Norgen's HLVd TaqMan RT-PCR Kit Related Products Plant/Fungi Total RNA Purification Kit
Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) TaqMan RT-PCR Detection Kits Price USD$949.00 SKU TM69950 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
