Features and Benefits

Multiplexed TaqMan PCR-based kit for Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) detection. Norgen's turn-key solution comes with Master Mix, Primer & Probe Mix, Positive control, and Nuclease free water. Norgen's TaqMan Master Mix includes an internal PCR control to monitor PCR inhibition and validate sample quality. Pair with Norgen's Plant/Fungi RNA Purification Kit for the highest level of sensitivity in detection.

This kit is designed for research use only and not for use in diagnostic procedures.

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Figure 1.  Example of TaqMan One-step RT-PCR Positive result. PCR signals above the baseline from FAM, Tex615, Cy5, and HEX channel indicate the successful PCR. Individual amplification in any of the channels also indicates a positive result for the pathogen corresponding to that channel.

Figure 2.  Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM,TEX615, and Cy5 channels but amplification signal from HEX indicates the successful PCR.

Figure 3.  Example of TaqMan one-step RT-PCR inhibition result. No signal from all targets was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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Documentation

Related Products

Plant/Fungi Total RNA Purification Kit