The Plant/Fungi total RNA kits that we purchase from Norgen have been great. We look forward to working with you and receiving quality products.
I am very glad that I decided to try your Norgen RNA extraction kit for plants.
I work with cotton and most of the Plant RNA extraction kits I have tried are unsuccessful obtaining RNA from cotton with good quality (A260 ratios) and yield.
The Norgen Plant RNA extraction kit successfully gave me good RNA yield and quality from cotton.
In the nearby future, I will be acquiring some more kits.
School of Integrative Plant Science Plant Pathology and Plant-Microbe Biology Section
Plant/Fungi Total RNA Purification Kit
For the rapid purification of total RNA (including microRNA) from plants and fungi
For research use only and NOT intended for in vitro diagnostics.
Plant/Fungi Total RNA Purification Kit (Cat. 25800)50 preps
Plant/Fungi Total RNA Purification Kit (Cat. 31350)100 preps
Plant/Fungi Total RNA Purification Kit (Cat. 25850)250 preps
Plant/Fungi Total RNA Purification 96-Well Kit (Cat. 31900)2 x 96-well plates
Format: Spin Column
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
Isolate total RNA (including microRNA) without phenol
Isolated RNA is of high quality, integrity and diversity
Also available in 96-well format for high throughput applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
Figure 1. Isolation of High Quality RNA, even from Difficult Samples. Total RNA was isolated from 50 mg samples of apple (Lanes 1), peach (Lanes 2), grape (Lanes 3), pine needle (Lanes 4), strawberry (Lanes 5) and pear (Lanes 6) using Norgen's kit and a competitors kit. Five microlitres of total RNA from the 50 µL elution was loaded on 1X MOPS 1.0 % Formaldehyde-Agarose RNA gel for analysis. Norgen's kit allowed for the isolation of high quality RNA from all the samples, including the difficult samples, while the competitor failed to isolate RNA from grape, pine needles and strawberry. Furthermore, only Norgen's kit was able to isolate the small RNA species (white box).