Plant/Fungi Total RNA Purification Kit (Cat. 25800, 31350, 25850, 31900)
Plant/Fungi Total RNA Purification Kit
![Plant/Fungi Total RNA Purification Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/images/product_images/25800-PlantFungiTotalRNA-web.jpg?itok=E_IZ2HDi" "Plant/Fungi Total RNA Purification Kit")
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The Plant/Fungi total RNA kits that we purchase from Norgen have been great. We look forward to working with you and receiving quality products.
- Syngenta
Plant/Fungi Total RNA Purification Kit
For the rapid purification of total RNA (including microRNA) from plants and fungi
- Extract total RNA, including virus & viroid RNA
- Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
- Isolate total RNA (including microRNA) without phenol
- Isolated RNA is of high quality, integrity and diversity
- Also available in 96-well format for high throughput applications
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 25800) 50 preps
(Cat. 31350) 100 preps
(Cat. 25850) 250 preps
(Cat. 31900) 2 x 96-well plates
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
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Please note that Norgen Biotek provides sample kits exclusively to residents of North America. Customers outside of North America may request a sample from one of our global distributors. Find your local distributor.
Kit Specifications
|
Kit Specifications - Spin Column
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi |
50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves |
60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
|
Kit Specifications - Spin Column
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi |
50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves |
60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
|
Kit Specifications - Spin Column
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi |
50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves |
60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
* Yield will vary depending on the type of sample processed.
|
Kit Specifications - 96-well
|
|
|
Maximum Binding Capacity Per Well
|
50 μg
|
|
Maximum Loading Volume Per Well
|
500 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
|
Maximum Amount of Starting Material: Fungi |
40 mg 40 mg |
| Average Yield* 40 mg of Grape Leaves 40 mg Tomato Leaves 40 mg Tobacco Leaves 40 mg Peach Leaves |
5-7 μg 20-30 μg 20-30 μg 15-20 μg |
| Time to Complete 96 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
Supporting Data
| Title | The expanding menagerie of Prunus-infecting luteoviruses |
| Journal | Phytopathology. 2022. |
| Authors | Maryam Khalili, Thierry Candresse, Igor Koloniuk, Dana Safarova, Yoann Brans, et al. |
| Title | Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa |
| Journal | Plant Molecular Biology. 2022. |
| Authors | Vida Nasrollahi, Ze-Chun Yuan, Qing Shi Mimmie Lu, Tim McDowell, Susanne E. Kohalmi & Abdelali Hannoufa |
| Title | Regulatory Roles of Small Non-coding RNAs in Sugar Beet Resistance Against Beet curly top virus |
| Journal | Front. Plant Sci. 2022. |
| Authors | Rajtilak Majumdar, Paul J. Galewski, Imad Eujayl, Rakesh Minocha, Eric Vincill and Carl A. Strausbaugh |
| Title | Identification and complete genome sequencing of a divergent olive virus T isolate and an olive leaf yellowing-associated virus isolate naturally infecting olive trees in Greece |
| Journal | Virus Genes. 2022. |
| Authors | Asimina Katsiani, Polina Panailidou, Matthaios Mathioudakis, Nikolaos Katis & Varvara I. Maliogka |
| Title | Apple Autotetraploids—Phenotypic Characterisation and Response to Drought Stress |
| Journal | Agronomy. 2022. |
| Authors | Danuta Wójcik, Monika Marat, Agnieszka Marasek-Ciołakowska, Krzysztof Klamkowski, Zbigniew Buler, Małgorzata Podwyszyńska, Przemysław Piotr Tomczyk, Katarzyna Wójcik, Waldemar Treder and Jacek Filipczak |
| Title | Differences in Gene Expression of Pear Selections Showing Leaf Curling or Leaf Reddening Symptoms Due to Pear Decline Phytoplasma |
| Journal | Plants (Basel). 2022. |
| Authors | Mina Kaviani, Paul H Goodwin, David M Hunter |
| Title | Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves during Exposure to Powdery Mildew |
| Journal | Turkish Journal of Agriculture. 2022. |
| Authors | Ulku Baykal, Kadriye Özcan |
| Title | miR160 Interacts in vivo With Pinus pinaster AUXIN RESPONSE FACTOR 18 Target Site and Negatively Regulates Its Expression During Conifer Somatic Embryo Development |
| Journal | Frontiers in Plant Science. 2022. |
| Authors | Alves A, Confraria A, Lopes S, Costa B, Perdiguero P, Milhinhos A, Baena-González E, Correia S, Célia M Miguel |
| Title | Octopamine receptor gene influences social grouping in the masked birch caterpillar |
| Journal | BMC Research Notes. 2022. |
| Authors | Chanchal Yadav, Jayne E. Yack & Myron L. Smith |
| Title | A revised view on the evolution of glutamine synthetase isoenzymes in plants |
| Journal | the plant journal. 2022. |
| Authors | José Miguel Valderrama-Martín, Francisco Ortigosa, Concepción Ávila, Francisco M. Cánovas, Bertrand Hirel, Francisco R. Cantón, Rafael A. Cañas |
| Title | Rye B chromosomes differently influence the expression of A chromosome–encoded genes depending on the host species |
| Journal | Chromosome Research. 2022. |
| Authors | Anastassia Boudichevskaia, Anne Fiebig, Katrin Kumke, Axel Himmelbach & Andreas Houben |
| Title | Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi |
| Journal | Front Plant Sci. 2022. |
| Authors | Marian Morcillo, Ester Sales, Elena Corredoira, María Teresa Martínez, Juan Segura, and Isabel Arrillaga |
| Title | Candida albicans Induces Cross-Kingdom miRNA Trafficking in Human Monocytes To Promote Fungal Growth |
| Journal | Host-Microbial Interactions. 2022. |
| Authors | Luke D. Halder, Svitlana Babych, Diana I. Palme, Elham Mansouri-Ghahnavieh, Lia Ivanov, Victory Ashonibare, Daniela Langenhorst |
| Title | First Report of Tomato Brown Rugose Fruit Virus in Tomato in Norway |
| Journal | APS Publications. 2022. |
| Authors | Zhibo Hamborg and Dag-Ragnar Blystad |
| Title | Occurrence of hypovirus-infected Cryphonectria parasitica isolates in northern Spain: an encouraging situation for biological control of chestnut blight in Asturian forests |
| Journal | European Journal of Plant Pathology. 2017. |
| Authors | Trapiello, E., Rigling, D., & González, A. J |
| Title | Analysis of Extracellular Vesicles Produced in the Biofilm by the Dimorphic Yeast Pichia fermentans |
| Journal | Journal of Cellular Physiology. 2017. |
| Authors | Leone F, Bellani L, Mucciflora S, Giorgetti L, Bongioanni P, Simili M, Maserti BE, Del Carratore R |
| Title | Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses |
| Journal | Scientific Reports. 2017. |
| Authors | Zhang Y, Han X, Chen S, Zheng L, He X, Liu M, Qiao G, Wang Y, Zhuo R |
| Title | Incidence and characterization of viruses infecting orchids in São Paulo state, Brazil |
| Journal | Tropical Plant Pathology. 2017. |
| Authors | Letícia A Moraes, Renate Krause-Sakate, Marcelo A. Pavan |
| Title | Increasing the synthesis of bioactive abietane diterpenes in Salvia sclarea hairy roots by elicited transcriptional reprogramming |
| Journal | Plant Cell Reports. 2016. |
| Authors | Vaccaro MC, Alfieri ME, Malafronte N, De Tommasi N, Leone A. |
| Title | Assessment of promoters and a selectable marker for development of strawberry intragenic vectors |
| Journal | Plant Cell, Tissue and Organ Culture (PCTOC). 2016. |
| Authors | Carvalho, R. F. and Folta, K. M |
| Title | A putative 3-hydroxyisobutyryl-CoA hydrolase is required for efficient symbiotic nitrogen fixation in Sinorhizobium meliloti and Sinorhizobium fredii NGR234 |
| Journal | Environmental Microbiology. 2016. |
| Authors | Zamani M, diCenzo GC, Milunovic B, Finan TM. |
| Title | De-novo transcriptome assembly for gene identification, analysis, annotation, and molecular marker discovery in Onobrychis viciifolia |
| Journal | BMC Genomics. 2016. |
| Authors | Mora-Ortiz M, Swain MT, Vickers MJ, Hegarty MJ, Kelly R, Smith LM, Skøt L. |
| Title | First report of Potato mop-top virus infecting potatoes in Alberta |
| Journal | Plant Disease. 2016. |
| Authors | Kalischuk, M., Lynn, J., & Kawchuk, L |
| Title | Multiple mutualist effects on genome-wide expression in the tripartite association between Medicago truncatula, nitrogen-fixing bacteria, and mycorrhizal fungi |
| Journal | Molecular Ecology. 2016. |
| Authors | Afkhami, M. E., & Stinchcombe, J. R |
| Title | Nickel and Copper Toxicity and Plant Response Mechanisms in White Birch (Betula papyrifera) |
| Journal | Bulletin of Environmental Contamination and Toxicology. 2016. |
| Authors | Theriault G, Nkongolo K |
| Title | The transcription factor NIN-LIKE PROTEIN 7 (NLP7) controls border-like cell release in Arabidopsis |
| Journal | Plant Phisiology. 2016. |
| Authors | Karve R, Suárez-Román F, Iyer-Pascuzzi AS |
| Title | Molecular characterization of a novel putative ampelovirus tentatively named grapevine leafroll-associated virus 13 |
| Journal | Archives of Virology. 2016. |
| Authors | Chiantore MV, Mangino G, Iuliano M, Zangrillo MS, De Lillis I, Vaccari G, Accardi R, Tommasino M, Columba Cabezas S, Federico M, Fiorucci G, Romeo G Ito T, Nakaune R |
| Title | Concerted genomic targeting of H3K27 demethylase REF6 and chromatin-remodeling ATPase BRM in Arabidopsis |
| Journal | Nature Genetics. 2016. |
| Authors | Li C, Gu L, Gao L, Chen C, Wei CQ, Qiu Q, Chien CW, Wang S, Jiang L, Ai LF, Chen CY, Yang S, Nguyen V, Qi Y, Snyder MP, Burlingame AL, Kohalmi SE, Huang S, Cao X, Wang ZY, Wu K, Chen X, Cui Y |
| Title | Auxin-mediated lamina growth in tomato leaves is restricted by two parallel mechanisms |
| Journal | The Plant Journal. 2016. |
| Authors | Ben-Gera H, Dafna A, Alvarez JP, Bar M, Mauerer M, Ori N |
| Title | Transcriptomic responses of mixed cultures of ascomycete fungi to lignocellulose using dual RNA-seq reveal inter-species antagonism and limited beneficial effects on CAZyme expression |
| Journal | Fungal Genetics and Biology. 2016. |
| Authors | Daly P, van Munster JM, Kokolski M, Sang F, Blythe MJ, Malla S, Oliveira JV, Goldman GH, Archer DB |
| Title | Comprehensive Transcriptome Analysis of Response to Nickel Stress in White Birch (Betula papyrifera) |
| Journal | PLOS ONE. 2016. |
| Authors | Theriault, G., Michael, P., & Nkongolo, K |
| Title | Decrypting the regulation and mechanism of nickel resistance in white birch (Betula papyrifera) using cross-species metal-resistance genes |
| Journal | Genes & Genomics. 2016. |
| Authors | Gabriel Theriault, Paul Michael, Kabwe Nkongolo |
| Title | Electrolyzed sodium bicarbonate inhibits Penicillium digitatum and induces defence responses against green mould in citrus fruit |
| Journal | Postharvest Biology and Technology. 2016. |
| Authors | Frida Fallanaj, Antonio Ippolito, Angela Ligorio, Francesca Garganese, Ciro Zavanella, Simona M. Sanzani |
| Title | Analysis of Microtubule-Associated-Proteins during IBA-Mediated Adventitious Root Induction Reveals KATANIN Dependent and Independent Alterations of Expression Patterns |
| Journal | PLOS ONE. 2015. |
| Authors | Mohamad Abu-Abied, Inna Mordehaev, Gujulla B Sunil Kumar, Ron Ophir, Geoffrey O. Wasteneys, Einat Sadot |
| Title | A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics |
| Journal | Virology Journal. 2015. |
| Authors | Huogen Xiao, Won-Sik Kim and Baozhong Meng |
| Title | Identification of conserved drought-adaptive genes using a cross-species meta-analysis approach |
| Journal | BMC Plant Biology. 2015. |
| Authors | Lidor Shaar-Moshe, Sariel Hübner and Zvi Peleg |
| Title | An assemblage of divergent variants of a novel putative closterovirus from American persimmon |
| Journal | Virus Genes. 2015. |
| Authors | Takao Ito, Akihiko Sato, Koichi Suzaki |
| Title | Evaluation of viroid extraction methods and application of a one-step reverse transcription real-time polymerase chain reaction assay (RT-qPCR) for the rapid detection of Chrysanthemum stunt viroid (CSVd) infection |
| Journal | Canadian Journal of Plant Pathology. 2015. |
| Authors | W.-S. Kim, Y. Haj-Ahmad, L. W. Stobbs & N. Greig |
| Title | COBRA-LIKE 2, a member of the GPI-anchored COBRA-LIKE family, plays a role in cellulose deposition in Arabidopsis seed coat mucilage secretory cells. |
| Journal | Plant Physiology Preview. 2015. |
| Authors | Ben-Tov D, Abraham Y, Stav S, Thompson K, Loraine A, Elbaum R, De Souza A, Pauly M, Kieber JJ, Harpaz-Saad S |
| Title | Priming with a double-stranded DNA virus alters Brassica rapa seed architecture and facilitates a defense response |
| Journal | Gene. 2014. |
| Authors | Melanie L. Kalischuk, Dan Johnson, Lawrence M. Kawchuk |
| Title | Sequence diversity and gene expression analyses of expansin-related proteins in the white-rot basidiomycete, Phanerochaete carnosa. |
| Journal | Fungal Genetics and Biology. 2014. |
| Authors | Hitoshi Suzuki, Thu V. Vuong, Yunchen Gong, Kin Chan, Chi-Yip Ho, Emma R. Master, Akihiko Kondo. |
| Title | Expression analysis of histone acetyltransferases in rice under drought stress. |
| Journal | Biochemical and Biophysical Research Communications. 2013. |
| Authors | Fang H, Liu X, Thorn G, Duan J, Tian L. |
| Title | Weak-acid preservatives: pH and proton movements in the yeast Saccharomyces cerevisiae. |
| Journal | International Journal of Food Microbiology. 2012. |
| Authors | Stratford M, Nebe-von-Caron G, Steels H, Novodvorska M, Ueckert J, Archer DB. |
| Title | Identification and genetic characterization of a gibberellin 2-oxidase gene that controls tree stature and reproductive growth in plum. |
| Journal | Journal of Experimental Botany. 2011. |
| Authors | El-Sharkawy I, El Kayal W, Prasath D, Fernˆndez H, Bouzayen M, Svircev AM, Jayasankar S. |
| Title | Analysis of Fungal Gene Expression by Real Time Quantitative PCR |
| Journal | Methods in Molecular Biology, Molecular and Cell Biology Methods for Fungi 103-114. Humana Press.. 2010. |
| Authors | Ish-Shalom1 S., Lichter A. |
| Title | Molecular characterization of seven genes encoding ethylene-responsive transcriptional factors during plum fruit development and ripening |
| Journal | The Journal of Experimental Botany.60: 907 - 922. 2009. |
| Authors | El-Sharkawy I, Sherif S, Mila I, Bouzayen M, Jayasankar S. |
| Title | Detection and genetic characterisation of a novel mycovirus in Hymenoscyphus fraxineus, the causal agent of ash dieback. |
| Journal | Infection, Genetics and Evolution. 2014. |
| Authors | Corine N. Schoebel, Stefan Zoller, Daniel Rigling. |
| Title | Development of a Pseudomonas syringae–Eutrema salsugineum pathosystem to investigate disease resistance in a stress tolerant extremophile model plant. |
| Journal | Plant Pathology. 2014. |
| Authors | MTS Yeo, P Carella, J Fletcher, MJ Champigny, EA Weretilnyk and Cameron |
| Title | Profiling microRNAs in Eucalyptus grandis reveals no mutual relationship between alterations in miR156 and miR172 expression and adventitious root induction during development. |
| Journal | BMC Genomics. 2014. |
| Authors | Aviv Levy, David Szwerdszarf, Mohamad Abu-Abied, Inna Mordehaev, Yossi Yaniv, Joseph Riov, Tzahi Arazi and Einat Sadot |
| Title | Enhanced biosynthesis of bioactive abietane diterpenes by overexpressing AtDXS or AtDXR genes in Salvia sclarea hairy roots. |
| Journal | Plant Cell, Tissue and Organ Culture (PCTOC). 2014. |
| Authors | Mariacarmela Vaccaro, Nicola Malafronte, Mariaevelina Alfieri, Nunziatina De Tommasi, Antonietta Leone |
| Title | The role of carbon starvation in the induction of enzymes that degrade plant-derived carbohydrates in Aspergillus niger. |
| Journal | Fungal Genetics Biology. 2014. |
| Authors | van Munster JM, Daly P, Delmas S, Pullan ST, Blythe MJ, Malla S, Kokolski M, Noltorp EC, Wennberg K, Fetherston R, Beniston R, Yu X, Dupree P, Archer DB. |
| Title | A Contig-Based Strategy for the Genome-Wide Discovery of MicroRNAs without Complete Genome Resources. |
| Journal | PLoS One. 2014. |
| Authors | Wen JZ, Liao JY, Zheng LL, Xu H, Yang JH, Guan DG, Zhang SM, Zhou H, Qu LH. |
| Title | The transcription factor SlSHINE3 modulates defense responses in tomato plants. |
| Journal | Plant Mol Biology. 2013. |
| Authors | Buxdorf K, Rubinsky G, Barda O, Burdman S, Aharoni A, Levy M. |
| Title | An Arabidopsis homolog of importin beta 1 is required for ABA response and drought tolerance. |
| Journal | The Plant Journal. 2013. |
| Authors | Luo Y, Wang Z, Ji H, Fang H, Wang S, Tian L, Li X. |
| Title | Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms. |
| Journal | Archives of Virology. 2013. |
| Authors | Ito T, Nakaune R, Nakano M, Suzaki K. |
| Title | Trancriptional landscape of Aspergillus niger at breaking of conidial dormancy revealed by RNA-sequencing. |
| Journal | BMC Genomics. 2013. |
| Authors | Novodvorska M, Hayer K, Pullan ST, Wilson R, Blythe MJ, Stam H, Stratford M, Archer DB. |
| Title | Graft-induced Changes in MicroRNA Expression Patterns in Citrus Leaf Petioles. |
| Journal | The Open Plant Science Journal. 2013. |
| Authors | R. Tzarfati, S.Ben-Dor, I. Sela and E.E. Goldschmidt |
| Title | Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum. |
| Journal | PLOS One. 2013. |
| Authors | Wang X, Kohalmi SE, Svircev A, Wang A, Sanfaçon H, Tian L. |
| Title | Gene expression indicates a zone of heterocyst differentiation within the thallus of the cyanolichen Pseudocyphellaria crocata. |
| Journal | New Phytologist. 2012 |
| Authors | Chua JP, Wallace EJ, Yardley JA, Duncan EJ, Dearden PK, Summerfield TC. |
| Title | A Survey of MicroRNA Length Variants Contributing to miRNome Complexity in Peach (Prunus Persica L.). |
| Journal | Frontiers in Plant Science. 2011 |
| Authors | Colaiacovo M, Bernardo L, Centomani I, Crosatti C, Giusti L, Orrù L, Tacconi G, Lamontanara A, Cattivelli L, Faccioli P. |
| Title | LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues. |
| Journal | BMC Research Notes. 2011 |
| Authors | Yaffe H, Buxdorf K, Shapira I, Ein-Gedy S, Moyal-Ben Zvi M, Fridman E, Moselion M, Levy M. |
| Title | Root colonisation of Pseudomonas aeruginosa strain UPMP3 and induction of defence-related genes in oil palm (Elaeis guineensis). |
| Journal | Annals of Applied Biology. 2011 |
| Authors | Sathyapriya H, Sariah M, Siti Nor Akmar A and Wong M. |
| Title | A classification of Pepper yellow mosaic virus isolates into pathotypes. |
| Journal | European Journal of Plant Pathology. 2011 |
| Authors | Moura M, Mituti T, Marubayashi J, Gioria R, Kobori R, Pavan M, Silva N, Krause-Sakate R. |
| Title | Regulation of two germin-like protein genes during plum fruit |
| Journal | Journal of Experimental Botony, Mar 4 2010. |
| Authors | El-Sharkawy I, Mila I, Bouzayen M and Jayasankar S. |
| Title | Arabidopsis DNA methyltransferase AtDNMT2 associates with histone deacetylase AtHD2s activity. |
| Journal | Biochemical and Biophysical Research Communications, p103-114. Mar 21 2010. |
| Authors | Song Y, Wu K, Dhaubhadel S, An L, Tian L. |
Plant/Fungi Total RNA Purification Kit
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
Supporting Data
Kit Specifications
|
Kit Specifications - Spin Column
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi |
50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves |
60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
|
Kit Specifications - Spin Column
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi |
50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves |
60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
|
Kit Specifications - Spin Column
|
|
|
Maximum Column Binding Capacity
|
50 μg
|
|
Maximum Column Loading Volume
|
650 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi |
50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves |
60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
* Yield will vary depending on the type of sample processed.
|
Kit Specifications - 96-well
|
|
|
Maximum Binding Capacity Per Well
|
50 μg
|
|
Maximum Loading Volume Per Well
|
500 μL
|
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt) |
|
Maximum Amount of Starting Material: Fungi |
40 mg 40 mg |
| Average Yield* 40 mg of Grape Leaves 40 mg Tomato Leaves 40 mg Tobacco Leaves 40 mg Peach Leaves |
5-7 μg 20-30 μg 20-30 μg 15-20 μg |
| Time to Complete 96 Purifications |
30 minutes
|
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
| Title | The expanding menagerie of Prunus-infecting luteoviruses |
| Journal | Phytopathology. 2022. |
| Authors | Maryam Khalili, Thierry Candresse, Igor Koloniuk, Dana Safarova, Yoann Brans, et al. |
| Title | Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa |
| Journal | Plant Molecular Biology. 2022. |
| Authors | Vida Nasrollahi, Ze-Chun Yuan, Qing Shi Mimmie Lu, Tim McDowell, Susanne E. Kohalmi & Abdelali Hannoufa |
| Title | Regulatory Roles of Small Non-coding RNAs in Sugar Beet Resistance Against Beet curly top virus |
| Journal | Front. Plant Sci. 2022. |
| Authors | Rajtilak Majumdar, Paul J. Galewski, Imad Eujayl, Rakesh Minocha, Eric Vincill and Carl A. Strausbaugh |
| Title | Identification and complete genome sequencing of a divergent olive virus T isolate and an olive leaf yellowing-associated virus isolate naturally infecting olive trees in Greece |
| Journal | Virus Genes. 2022. |
| Authors | Asimina Katsiani, Polina Panailidou, Matthaios Mathioudakis, Nikolaos Katis & Varvara I. Maliogka |
| Title | Apple Autotetraploids—Phenotypic Characterisation and Response to Drought Stress |
| Journal | Agronomy. 2022. |
| Authors | Danuta Wójcik, Monika Marat, Agnieszka Marasek-Ciołakowska, Krzysztof Klamkowski, Zbigniew Buler, Małgorzata Podwyszyńska, Przemysław Piotr Tomczyk, Katarzyna Wójcik, Waldemar Treder and Jacek Filipczak |
| Title | Differences in Gene Expression of Pear Selections Showing Leaf Curling or Leaf Reddening Symptoms Due to Pear Decline Phytoplasma |
| Journal | Plants (Basel). 2022. |
| Authors | Mina Kaviani, Paul H Goodwin, David M Hunter |
| Title | Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves during Exposure to Powdery Mildew |
| Journal | Turkish Journal of Agriculture. 2022. |
| Authors | Ulku Baykal, Kadriye Özcan |
| Title | miR160 Interacts in vivo With Pinus pinaster AUXIN RESPONSE FACTOR 18 Target Site and Negatively Regulates Its Expression During Conifer Somatic Embryo Development |
| Journal | Frontiers in Plant Science. 2022. |
| Authors | Alves A, Confraria A, Lopes S, Costa B, Perdiguero P, Milhinhos A, Baena-González E, Correia S, Célia M Miguel |
| Tit |