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  • Rapid and sensitive tool for measuring the efficiency of homologous recombination
  • For any cell types including prokaryotes and eukaryotes
  • All primers included for quantification of the homologous recombination efficiency through qPCR.

Rapid and sensitive tool for measuring the efficiency of homologous recombination

ItemsCat. #Size
Homologous Recombination Assay Kit 35600

  • Rapid and sensitive tool for measuring the efficiency of homologous recombination
  • For any cell types including prokaryotes and eukaryotes
  • All primers included for quantification of the homologous recombination efficiency through qPCR.


Homologous Recombination Assay Kit

Norgen’s Homologous Recombination Assay Kit provides a rapid and sensitive tool for measuring the efficiency of homologous recombination in both bacterial and mammalian cells. The kit consists of 5 components: (1) dl-1 plasmid, (2) dl-2 plasmid, (3) positive control plasmid, (4) assay primers mixture, and (5) universal primers mixture. The assay is based on the co-transformation of the two dl plasmids into bacterial cells followed by the isolation of plasmid DNA and quantification of the homologous recombination efficiency through qPCR. In addition, PCR can be used for qualitative analysis of the recombination efficiency. The primer mixture provided with the kit is designed to amplify only the homologous recombination product but not the dl plasmids (dl-1 or dl-2). The two plasmids can be co-transfected into mammalian cells, then the DNA can be isolated and the homologous recombination efficiency can be assayed by qPCR (or PCR). In addition, the efficiency of homologous recombination can be assessed in bacteria by blue/white screening using X-gal. The provided positive control contains the functional LacZ sequence and can be used as a positive control for the plasmid DNA isolation efficiency (after transformation or transfection) or PCRs. The use of PCR-based homologous recombination assay enhances the sensitivity and eliminates the need for labor intensive screening by using traditional protein expression and staining techniques and is suitable for high throughput screening studies.  The kit is designed to perform 200 transformations or 24 transfections.

  • Rapid and sensitive tool - All primers included to allos for measuring the efficiency of homologous recombination through PCR or qPCR
  • For any cell types - Kit can be used to measure efficiency of homolgous recombination in both prokaryotes and eukaryotes

Homologous Recombination Assay Kit Contents:
1.  dl-1 Plasmid
2.  dl-2 Plasmid
3.  Positive Control Plasmid
4.  Assay Primers Mixture
5.  Universal Primers Mixture
6.  Nuclease-Free Water
7.  Product Insert

SHELF LIFE AND HANDLING
All solutions should be kept tightly sealed and stored at -20oC or lower temperature for up to 1 year without any reduction in kit performance.


Protocols: 
Safety Data Sheets: 

Title Hepatocyte Growth Factor Improves the Therapeutic Efficacy of Human Bone Marrow Mesenchymal Stem Cells via RAD51
Journal Molecular Therapy. 2018.
Authors Eun Ju Lee, Injoo Hwang, Ji Yeon Lee, Jong Nam Park, Keun Cheon Kim, Gi-Hwam Kim, Chang-Mo Kang, Irene Kim, Seo-Yeon Lee, Hyo-Soo Kim
Title Significance of cyclin D1 overexpression in progression and radio-resistance of pediatric ependymomas
Journal Oncotarget. 2017.
Authors Muh-Lii Liang, Tsung-Han Hsieh, Yun-Ru Liu, Yi-Wei Chen, Yi-Yen Lee, Feng-Chi Chang, Shih-Chiej Lin, Ming-Chao Huang, Donald Ming-Tak Ho, Tai-Tong Wong, Yun Yen, and Muh-Hwa Yang
Title Trifluoperazine, a novel autophagy inhibitor, increases radiosensitivity in glioblastoma by impairing homologous recombination
Journal Journal of Experimental & Clinical Research. 2017.
Authors Xin Zhang, Ran Xu, Chao Zhang, Yangyang Xu, Mingzhi Han, Bin Huang, Anjing Chen, Chen Qui, Frits Thorsenm Lars Prestegarden, Rolf Bjerkvig, Jian Wang and Xingang Li
Title Effect of endonuclease G depletion on plasmid DNA uptake and levels of homologous recombination in hela cells
Journal Molecular Cell Biology. 2016.
Authors V. Misic, M. El-Mogy, S. Geng, Y. Haj-Ahmad
Title 14-3-3sigma Contributes to Radioresistance by Regulating DNA Repair and Cell Cycle via PARP1 and CHK2
Journal Molecular Cancer Research. 2017.
Authors Chen Y, Li Z, Dong Z, Beebe J, Yang K, Fu L, Zhang JT.
Title Protein–Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays
Journal Small Molecule Microarrays. 2016.
Authors Na Z, Pan S, Uttamchandani M, Yao SQ.
Title Hypoxia Differentially Modulates the Genomic Stability of Clinical-Grade ADSCs and BM-MSCs in Long-Term Culture
Journal Stem Cells. 2015
Authors Bigot N, Mouche A, Preti M, Loisel S, Renoud ML, Le Guével R, Sensebé L, Tarte K, Pedeux R
Title Targeting MPS1 Enhances Radiosensitization of Human Glioblastoma by Modulating DNA Repair Proteins.
Journal Molecular Cancer Research. 2015
Authors Maachani UB, Kramp T, Hanson R, Zhao S, Celiku O, Shankavaram U, Colombo R, Caplen NJ, Camphausen K, Tandle A
Title Mutant IDH1-driven cellular transformation increases RAD51-mediated homologous recombination and temozolomide resistance.
Journal Cancer Research. 2014.
Authors Ohba S, Mukherjee J, See WL, Pieper RO.

ItemsCat. #Size
Homologous Recombination Assay Kit 35600

  • Rapid and sensitive tool for measuring the efficiency of homologous recombination
  • For any cell types including prokaryotes and eukaryotes
  • All primers included for quantification of the homologous recombination efficiency through qPCR.


Homologous Recombination Assay Kit

Norgen’s Homologous Recombination Assay Kit provides a rapid and sensitive tool for measuring the efficiency of homologous recombination in both bacterial and mammalian cells. The kit consists of 5 components: (1) dl-1 plasmid, (2) dl-2 plasmid, (3) positive control plasmid, (4) assay primers mixture, and (5) universal primers mixture. The assay is based on the co-transformation of the two dl plasmids into bacterial cells followed by the isolation of plasmid DNA and quantification of the homologous recombination efficiency through qPCR. In addition, PCR can be used for qualitative analysis of the recombination efficiency. The primer mixture provided with the kit is designed to amplify only the homologous recombination product but not the dl plasmids (dl-1 or dl-2). The two plasmids can be co-transfected into mammalian cells, then the DNA can be isolated and the homologous recombination efficiency can be assayed by qPCR (or PCR). In addition, the efficiency of homologous recombination can be assessed in bacteria by blue/white screening using X-gal. The provided positive control contains the functional LacZ sequence and can be used as a positive control for the plasmid DNA isolation efficiency (after transformation or transfection) or PCRs. The use of PCR-based homologous recombination assay enhances the sensitivity and eliminates the need for labor intensive screening by using traditional protein expression and staining techniques and is suitable for high throughput screening studies.  The kit is designed to perform 200 transformations or 24 transfections.

  • Rapid and sensitive tool - All primers included to allos for measuring the efficiency of homologous recombination through PCR or qPCR
  • For any cell types - Kit can be used to measure efficiency of homolgous recombination in both prokaryotes and eukaryotes

Homologous Recombination Assay Kit Contents:
1.  dl-1 Plasmid
2.  dl-2 Plasmid
3.  Positive Control Plasmid
4.  Assay Primers Mixture
5.  Universal Primers Mixture
6.  Nuclease-Free Water
7.  Product Insert

SHELF LIFE AND HANDLING
All solutions should be kept tightly sealed and stored at -20oC or lower temperature for up to 1 year without any reduction in kit performance.

Protocols: 
Safety Data Sheets: 

Title Hepatocyte Growth Factor Improves the Therapeutic Efficacy of Human Bone Marrow Mesenchymal Stem Cells via RAD51
Journal Molecular Therapy. 2018.
Authors Eun Ju Lee, Injoo Hwang, Ji Yeon Lee, Jong Nam Park, Keun Cheon Kim, Gi-Hwam Kim, Chang-Mo Kang, Irene Kim, Seo-Yeon Lee, Hyo-Soo Kim
Title Significance of cyclin D1 overexpression in progression and radio-resistance of pediatric ependymomas
Journal Oncotarget. 2017.
Authors Muh-Lii Liang, Tsung-Han Hsieh, Yun-Ru Liu, Yi-Wei Chen, Yi-Yen Lee, Feng-Chi Chang, Shih-Chiej Lin, Ming-Chao Huang, Donald Ming-Tak Ho, Tai-Tong Wong, Yun Yen, and Muh-Hwa Yang
Title Trifluoperazine, a novel autophagy inhibitor, increases radiosensitivity in glioblastoma by impairing homologous recombination
Journal Journal of Experimental & Clinical Research. 2017.
Authors Xin Zhang, Ran Xu, Chao Zhang, Yangyang Xu, Mingzhi Han, Bin Huang, Anjing Chen, Chen Qui, Frits Thorsenm Lars Prestegarden, Rolf Bjerkvig, Jian Wang and Xingang Li
Title Effect of endonuclease G depletion on plasmid DNA uptake and levels of homologous recombination in hela cells
Journal Molecular Cell Biology. 2016.
Authors V. Misic, M. El-Mogy, S. Geng, Y. Haj-Ahmad
Title 14-3-3sigma Contributes to Radioresistance by Regulating DNA Repair and Cell Cycle via PARP1 and CHK2
Journal Molecular Cancer Research. 2017.
Authors Chen Y, Li Z, Dong Z, Beebe J, Yang K, Fu L, Zhang JT.
Title Protein–Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays
Journal Small Molecule Microarrays. 2016.
Authors Na Z, Pan S, Uttamchandani M, Yao SQ.
Title Hypoxia Differentially Modulates the Genomic Stability of Clinical-Grade ADSCs and BM-MSCs in Long-Term Culture
Journal Stem Cells. 2015
Authors Bigot N, Mouche A, Preti M, Loisel S, Renoud ML, Le Guével R, Sensebé L, Tarte K, Pedeux R
Title Targeting MPS1 Enhances Radiosensitization of Human Glioblastoma by Modulating DNA Repair Proteins.
Journal Molecular Cancer Research. 2015
Authors Maachani UB, Kramp T, Hanson R, Zhao S, Celiku O, Shankavaram U, Colombo R, Caplen NJ, Camphausen K, Tandle A
Title Mutant IDH1-driven cellular transformation increases RAD51-mediated homologous recombination and temozolomide resistance.
Journal Cancer Research. 2014.
Authors Ohba S, Mukherjee J, See WL, Pieper RO.

 

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