HPV 6/16 TaqMan Probe/Primer and Control Set (Cat. TM42010)100 rxns
Format: PCR
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Overview
Human Papillomavirus (HPV) 6/16 TaqMan PCR Detection Kits
Detection kits for the HPV 6/16
Available in TaqMan format for analysis
More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.
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HPV 6/16 TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HPV 6/16 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals (FAM and Cy5) above the baseline from FAM, Cy5 and HEX channel indicate the successful PCR.
TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Positive (Cy5) result. Both PCR signals above the baseline from Cy5 and HEX channel indicate the successful PCR.
TaqMan PCR Kit - Figure 3. Example of TaqMan PCR Positive (FAM) result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.
TaqMan PCR Kit - Figure 4. Example of TaqMan PCR Negative result. No target DNA was detected in FAM and Cy5 channel but amplification signal from HEX indicates the successful PCR.
TaqMan PCR Kit - Figure 5. Example of TaqMan PCR inhibition result. No signal from FAM, Cy5 and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.