Swab Collection and DNA Preservation System (Cat. 45690-B)

Swab Collection and DNA Preservation System

Cat. 45690-B

Swab Collection and DNA Preservation Kit

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Swab Collection and DNA Preservation System

Cat. 45690-B

For collection, ambient storage and transport of DNA from samples collected using a swab

Collect from nasal, buccal, saliva, fecal, skin, surfaces, and more
Completely stabilize microbiota profiles from the point-of-collection
Minimize bias in data analysis: NGS (Metagenomics/Microbiome), PCR and microarrays
No need to immediately process samples: ship and store at ambient temperatures
DNA preservation at room temperature over 2 years
Renders sample non-infectious for safe and easy shipping
Compatible with DNA isolation kits and protocols: Optimized isolation procedure using Norgen's Microbiome DNA Isolation Kit

For research use only and NOT intended for in vitro diagnostics.

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Swab Collection and DNA Preservation System (Bulk Format)
(Cat. 45690-B) 50 Units

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Kit Specifications

You have selected: Cat. 45690-B

Kit Specifications
Volume of Preservative	1.2 mL
Preservation Temperature	Room Temperature
Preservation time	2 years at room temperature

Shelf Life and Handling

When stored at room temperature, unused Norgen Swab Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.

Supporting Data

Figure 1. Stability of DNA Preserved in Norgen's Swab DNA Preservative at Room Temperature for up to 75 Months. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Swab DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 75 months. Saliva DNA was subsequently isolated at 1 week, 3 weeks, 1 month, 2 months, 4 months, 6 months, 8 months, 12 months, 20 months, 32 months, 40 months, 52 months and 75 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 75 months at ambient temperatures in Norgen's Swab DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 75 month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).

Figure 2. Detection of GAPDH Using Taqman Real-time PCR from the Saliva Samples Stored at Room Temperature from 1 Week Up to 75 Months. The quality of the saliva DNA stored at room temperature did not change from 1 week to 75 months, as indicated by the fact that the Ct values remained consistent. The improvement in the Ct values from the real-time PCR graph (red) also indicate the impact of the improved saliva DNA isolation method provided by the Saliva DNA Isolation Kit (Cat. RU45400).

Figure 3. Real Time PCR Amplification of Saliva DNA from the saliva samples stored at room temperature from 1 week up to 75 months (13 time points). All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition. Blue: Saliva DNA isolated using improved Saliva DNA Isolation Kit (Cat. RU45400). Black: No template control.

Figure 4. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Swab DNA Preservative. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.

Click for expanded view

Component	Cat. 45690 (50 units)
Swab Collection and DNA Preservation Kit	50
Product Insert	1

Protocols:

(45690-B) Swab Collection and DNA Preservation System - Protocol (50 units)

Supplementary Protocols:

45690 - Donor Procedure

45690 - Isolation of DNA from Norgen’s Swab Collection and DNA Preservation System using Norgen’s Saliva DNA Isolation Kit

Application Notes:

The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit

Inhibitory Effect of Norgen’s Saliva DNA Preservative on the Growth of Bacteria and Yeast

Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative

Safety Data Sheets:

(45690-B) Swab Collection and DNA Preservation System - Safety Data Sheet

Posters:

In‐Depth Analysis of the Microbial Diversity in Saliva Samples Preserved at Ambient Temperature for over 6 Years - PAG 2016

Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives

Product Flyers:

Swab Collection and DNA Preservation System

Customize Your Product

Title	Impact of Raised without Antibiotics Measures on Antimicrobial Resistance and Prevalence of Pathogens in Sow Barns
Journal	Antibiotics.2022.
Authors	Alvin C. Alvarado, Samuel M. Chekabab, Bernardo Z. Predicala and Darren R. Korber

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Swab Collection and DNA Preservation System

Norgen’s Swab Collection and DNA Preservation System is designed for collection, ambient storage and transport of DNA from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Swab Collection and DNA Preservation System contains Norgen’s Swab Preservative in a liquid format. The user simply collects the specimen using the provided swab, and then transfers the swab into the Swab Preservative. The Swab Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Swab Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Swab Preservative allow DNA samples to be stored at room temperature for over 2 years.

DNA Isolation from Preservative

Swab samples collected and preserved using Norgen’s Swab Collection and DNA Preservation System are compatible with most DNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA isolation kits and reagents. An optimized isolation procedure is available as part of Norgen’s Microbiome DNA Isolation Kit (Cat. 64100).

Supporting Data

Figure 1. Stability of DNA Preserved in Norgen's Swab DNA Preservative at Room Temperature for up to 75 Months. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Swab DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 75 months. Saliva DNA was subsequently isolated at 1 week, 3 weeks, 1 month, 2 months, 4 months, 6 months, 8 months, 12 months, 20 months, 32 months, 40 months, 52 months and 75 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 75 months at ambient temperatures in Norgen's Swab DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 75 month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).

Figure 2. Detection of GAPDH Using Taqman Real-time PCR from the Saliva Samples Stored at Room Temperature from 1 Week Up to 75 Months. The quality of the saliva DNA stored at room temperature did not change from 1 week to 75 months, as indicated by the fact that the Ct values remained consistent. The improvement in the Ct values from the real-time PCR graph (red) also indicate the impact of the improved saliva DNA isolation method provided by the Saliva DNA Isolation Kit (Cat. RU45400).

Figure 3. Real Time PCR Amplification of Saliva DNA from the saliva samples stored at room temperature from 1 week up to 75 months (13 time points). All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition. Blue: Saliva DNA isolated using improved Saliva DNA Isolation Kit (Cat. RU45400). Black: No template control.

Figure 4. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Swab DNA Preservative. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.

Click for expanded view

Kit Specifications

You have selected: Cat. 45690-B

Kit Specifications
Volume of Preservative	1.2 mL
Preservation Temperature	Room Temperature
Preservation time	2 years at room temperature

Shelf Life and Handling

When stored at room temperature, unused Norgen Swab Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.

Component	Cat. 45690 (50 units)
Swab Collection and DNA Preservation Kit	50
Product Insert	1

Protocols:

(45690-B) Swab Collection and DNA Preservation System - Protocol (50 units)

Supplementary Protocols:

45690 - Donor Procedure

45690 - Isolation of DNA from Norgen’s Swab Collection and DNA Preservation System using Norgen’s Saliva DNA Isolation Kit

Application Notes:

The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit

Inhibitory Effect of Norgen’s Saliva DNA Preservative on the Growth of Bacteria and Yeast

Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative

Safety Data Sheets:

(45690-B) Swab Collection and DNA Preservation System - Safety Data Sheet

Posters:

In‐Depth Analysis of the Microbial Diversity in Saliva Samples Preserved at Ambient Temperature for over 6 Years - PAG 2016

Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives

Product Flyers:

Swab Collection and DNA Preservation System

Customize Your Product

Title	Impact of Raised without Antibiotics Measures on Antimicrobial Resistance and Prevalence of Pathogens in Sow Barns
Journal	Antibiotics.2022.
Authors	Alvin C. Alvarado, Samuel M. Chekabab, Bernardo Z. Predicala and Darren R. Korber

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Swab Collection and DNA Preservation System (Cat. 45690-B)

Swab Collection and DNA Preservation System

Swab Collection and DNA Preservation System

Product Size

Request a Product Sample

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At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Swab Collection and DNA Preservation System (Cat. 45690-B)

Swab Collection and DNA Preservation System

Swab Collection and DNA Preservation System

Product Size

Request a Product Sample

Product Overview

Documentation

Citations

Related Products

Microbiome DNA Isolation Kit

Shipping Accessories - 50 Mailers

Genomic DNA Isolation Kit

Saliva DNA Isolation Kit

Cells and Tissue DNA Isolation Kits