microRNA (cel-miR-39) Spike-In Kit A quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction procedures and subsequent normalization in RT-qPCR assays For research use only and NOT intended for in vitro diagnostics. Powered by Bioz microRNA (cel-miR-39) Spike-In Kit A quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction procedures and subsequent normalization in RT-qPCR assays SKU 59000 Size 1 Kit Contact Distributor Overview Details Documentation Citations Overview Well-accepted microRNA sequence used for normalization in gene expression studies Best suited for RNA purification from samples with low RNA abundance including liquid biopsies (plasma/serum/urine etc.) and low cell or tissue inputs Compatible to expression analysis using RT-qPCR - both RNA and matching forward PCR primer provided. Fully compatible to Norgen's microScript cDNA Synthesis system Fully compatible to Next Generation Sequencing (Small RNA-Seq) library preparation workflow The amount of RNA that can be extracted from different biological or clinical samples varies greatly. For example, while a few micrograms of RNA could be easily purified from tissues and cells in excess amounts (such as from a few milligrams of tissue), many liquid biopsy samples may yield very low amounts of RNA. In fact, samples such as urine or plasma may yield 1 - 100 ng or less RNA per 100 µL of sample. Such a range of RNA quantity is often below the detection limit of most commonly used techniques for measuring RNA including nano-spectrophotometry and fluorescent nucleic acid stains. As a result, without properly determined RNA concentration, it becomes very difficult to normalize the starting quantity of RNA used in gene expression studies. Norgen’s microRNA (cel-miR-39) Spike-In Kit offers a quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction procedures and subsequent normalization in RT-qPCR assays. The amount of cel-miR-39 RNA recovered after RNA extraction is directly correlated with the amount of total RNA recovered. After reverse transcription (such as with Norgen’s microScript Reverse Transcription system) of the sample RNA (with spike-in), the level of cel-miR-39 can be determined by subjecting the cDNA generated to quantitative PCR (qPCR) using fluorescent nucleic acid stains such as SYBR Green. A cel-miR-39 specific primer is included in the kit. The level of expression of any target transcripts in different RNA samples can now be normalized to the cel-miR-39 transcript level using standard method such as ∆∆Ct relative quantification. In addition, the cel-miR-39 RNA is compatible to library preparation methods (including ligation-based protocols) in Next Generation Sequencing (Small RNA-Seq) workflows. The cel-miR-39 RNA could be used for normalization as well as for tracking library construction efficiency. Details Supporting Data Previous Figure 1. Accurate Quantity and High Quality cel-miR-39 Spike-In. Various quantities of the reconstituted cel-miR-39 RNA was used as spike-in during isolation of urinary RNA (1 mL) using Norgen's Urine Exosome RNA Isolation Kit (Cat. 47200). Isolated RNA was reverse transcribed using Norgen's microScript cDNA Synthesis Kit (Cat. 54410), followed by qPCR using Norgen's 2X PCR Master Mix (Cat. 28007), supplemented with SYBR Green I. The cel-miR-39 RNA was successfully detected in all samples, with the Ct values distributed according to the amount of RNA spiked-in. Figure 2. Successful Incorporation of cel-miR-39 Spike-In into Small RNA-Seq Library. Small RNA-Seq Library was generated using either a competitor’s or Norgen's cel-miR-39 Spike-In RNA at two concentrations (High and Low). The library was generated using Illumina's TruSeq Small RNA Library Preparation Kit. The incorporated libraries were resolved on a PAGE gel. Only Norgen's cel-miR-39 RNA showed effective incorporation into small RNA-Seq library (Red Arrows). The competitor's cel-miR-39 RNA only yielded bands (Yellow Arrows) equivalent to the No Template Control (NTC) that are adapter dimers. Next Click for expanded view Storage Conditions Upon receipt, store Norgen’s microRNA (cel-miR-39) Spike-In Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower. Component Cat. 59000 cel-miR-39 RNA 10 pmol cel-miR-39 Forward PCR Primer 1 nmol Nuclease-Free Water 1.25 mL Product Insert 1 Documentation PROTOCOLS cel-miR-39-Spike-In-Kit-Insert-PI59000-1.pdf SAFETY DATA SHEETS 59000_SDS.pdf Citations Powered by Bioz See more details on Bioz Related Services NGS Related Products 2X PCR Master Mix microScript microRNA cDNA Synthesis Kit Total RNA Purification Kit Plasma/Serum RNA Purification Kit Urine Cell-Free Circulating RNA Purification Mini Kit
microRNA (cel-miR-39) Spike-In Kit A quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction procedures and subsequent normalization in RT-qPCR assays For research use only and NOT intended for in vitro diagnostics. Powered by Bioz microRNA (cel-miR-39) Spike-In Kit A quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction procedures and subsequent normalization in RT-qPCR assays SKU 59000 Size 1 Kit Contact Distributor