MidRanger 1 kb DNA Ladder (Cat. 11700)
MidRanger 1 kb DNA Ladder
Cat. 11700
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MidRanger 1 kb DNA Ladder
Cat. 11700
Ideal for sizing larger PCR products (>1kb) and most cloning applications.
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Ten discrete fragments ranging from 300Â bp to 5000Â bp
For research use only and NOT intended for in vitro diagnostics.
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MidRanger 1 kb DNA Ladder
(Cat. 11700) 100 loads
(Cat. 11700) 100 loads
Product Size
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Kit Specifications
You have selected: Cat. 11700
MidRanger 1kb DNA Ladder (Cat# 11700) - 100 loads
Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp
• Ten discrete fragments ranging from 300 bp to 5000 bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
5000
|
88
|
|
2
|
4000
|
78
|
|
3
|
3000
|
67
|
|
4
|
2500
|
58
|
|
5
|
2000
|
50
|
|
6
|
1500
|
43
|
|
7
|
1000
|
25 |
|
8
|
700
|
34
|
|
9
|
500
|
33
|
|
10
|
300
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Supporting Data
Click for expanded view
Protocols:
Application Notes:
Safety Data Sheets:
| Title | Structural pattern and genetic diversity in blueberry (Vaccinium) clones and cultivars using EST-PCR and microsatellite markers |
| Journal | Genetic Resources and Crop Evolution. 2017 |
| Authors | Sapan Tailor, Natalia V. Bykova, Abir U. Igamberdiev, Samir C. Debnath |
| Title | Identification of genes for engineering the male germline of Aedes aegypti and Ceratitis capitata |
| Journal | BMC Genomics. 2016. |
| Authors | Sutton ER, Yu Y, Shimeld SM, White-Cooper H, Alphey AL |
| Title | The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres. |
| Journal | Genes and Development. 2012. |
| Authors | Chambers AL, Ormerod G, Durley SC, Sing TL, Brown GW, Kent NA, Downs JA. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
| Title | Unraveling a mechanism of honey antibacterial action: Polyphenol/H2O2-induced oxidative effect on bacterial cell growth and on DNA degradation. |
| Journal | Food Chemistry. 2012. |
| Authors | Brudzynski K, Abubaker K, Miotto D. |
| Title | Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. |
| Journal | Frontiers in Microbiology. 2011. |
| Authors | Brudzynski K, Abubaker K, Laurent M, Castle A. |
MidRanger 1 kb DNA Ladder
The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300Â bp to 5000Â bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Supporting Data
Click for expanded view
Kit Specifications
You have selected: Cat. 11700
MidRanger 1kb DNA Ladder (Cat# 11700) - 100 loads
Ladder Properties:
• Ten discrete fragments ranging from 300 bp to 5000 bp
• Ten discrete fragments ranging from 300 bp to 5000 bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
5000
|
88
|
|
2
|
4000
|
78
|
|
3
|
3000
|
67
|
|
4
|
2500
|
58
|
|
5
|
2000
|
50
|
|
6
|
1500
|
43
|
|
7
|
1000
|
25 |
|
8
|
700
|
34
|
|
9
|
500
|
33
|
|
10
|
300
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Protocols:
Application Notes:
Safety Data Sheets:
| Title | Structural pattern and genetic diversity in blueberry (Vaccinium) clones and cultivars using EST-PCR and microsatellite markers |
| Journal | Genetic Resources and Crop Evolution. 2017 |
| Authors | Sapan Tailor, Natalia V. Bykova, Abir U. Igamberdiev, Samir C. Debnath |
| Title | Identification of genes for engineering the male germline of Aedes aegypti and Ceratitis capitata |
| Journal | BMC Genomics. 2016. |
| Authors | Sutton ER, Yu Y, Shimeld SM, White-Cooper H, Alphey AL |
| Title | The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres. |
| Journal | Genes and Development. 2012. |
| Authors | Chambers AL, Ormerod G, Durley SC, Sing TL, Brown GW, Kent NA, Downs JA. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
| Title | Unraveling a mechanism of honey antibacterial action: Polyphenol/H2O2-induced oxidative effect on bacterial cell growth and on DNA degradation. |
| Journal | Food Chemistry. 2012. |
| Authors | Brudzynski K, Abubaker K, Miotto D. |
| Title | Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. |
| Journal | Frontiers in Microbiology. 2011. |
| Authors | Brudzynski K, Abubaker K, Laurent M, Castle A. |