Ideal for sizing larger PCR products (>1kb) and most cloning applications.

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Ten discrete fragments ranging from 300bp to 5000bp

Items Cat. # Size
MidRanger 1kb DNA Ladder 11700 100 loads

MidRanger 1 kb DNA Ladder

The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300bp to 5000bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise

MidRanger 1kb DNA Ladder (Cat# 11700) - 100 loads

Ladder Properties:
• Ten discrete fragments ranging from 300bp to 5000bp

 

Fragment
Size (bp)
Mass (ng)
1
5000
88
2
4000
78
3
3000
67
4
2500
58
5
2000
50
6
1500
43
7
1000

25 

8
700
34
9
500
33
10
300
24
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
 
Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
 

 

Title The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres.
Journal Genes and Development. 2012.
Authors Chambers AL, Ormerod G, Durley SC, Sing TL, Brown GW, Kent NA, Downs JA.
Title Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey.
Journal Frontiers in Microbiology. 2011.
Authors Brudzynski K, Abubaker K, Laurent M, Castle A.